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Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells

  • Authors:
    • Liyong Huang
    • Ye Xu
    • Guoxiang Cai
    • Zuqing Guan
    • Sanjun Cai
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, 270 Dong'an Road, Shanghai 200032, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 917-922
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    Published online on: December 20, 2011
       https://doi.org/10.3892/or.2011.1598
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Abstract

S100A4 protein, a member of the S100 superfamily of calcium-binding proteins, is frequently observed in various types of human cancers, including colorectal cancer (CRC). Our previous investigations have demonstrated that the overexpression of S100A4 is associated with lymph node metastasis and poor prognosis in CRC; however, its biological roles in CRC remain unclear. In the present study, we compared the expression of S100A4 at the mRNA and protein levels in six CRC cell lines, and found that the expression levels roughly coincided with their invasiveness. Using RNA interference, we suppressed S100A4 expression in SW620 CRC cells with highly invasive potential and S100A4 high expression. The specific knockdown of S100A4 strongly suppressed cell growth, migration and invasion activities. Furthermore, employing metastasis-related gene mRNA microarrays, we found four genes to be significantly dysregulated (more than 2-fold) after downregulation of S100A4, including three downregulated genes (MMP9, MMP10 and CDH11) and one upregulated gene (TIMP4). Our present results indicate that S100A4 may positively regulate tumor cell proliferation, invasion and metastasis associated with multiple molecules. Thus, the inhibition of S100A4 might be a potentially novel approach to treatment for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Huang L, Xu Y, Cai G, Guan Z and Cai S: Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells. Oncol Rep 27: 917-922, 2012.
APA
Huang, L., Xu, Y., Cai, G., Guan, Z., & Cai, S. (2012). Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells. Oncology Reports, 27, 917-922. https://doi.org/10.3892/or.2011.1598
MLA
Huang, L., Xu, Y., Cai, G., Guan, Z., Cai, S."Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells". Oncology Reports 27.4 (2012): 917-922.
Chicago
Huang, L., Xu, Y., Cai, G., Guan, Z., Cai, S."Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells". Oncology Reports 27, no. 4 (2012): 917-922. https://doi.org/10.3892/or.2011.1598
Copy and paste a formatted citation
x
Spandidos Publications style
Huang L, Xu Y, Cai G, Guan Z and Cai S: Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells. Oncol Rep 27: 917-922, 2012.
APA
Huang, L., Xu, Y., Cai, G., Guan, Z., & Cai, S. (2012). Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells. Oncology Reports, 27, 917-922. https://doi.org/10.3892/or.2011.1598
MLA
Huang, L., Xu, Y., Cai, G., Guan, Z., Cai, S."Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells". Oncology Reports 27.4 (2012): 917-922.
Chicago
Huang, L., Xu, Y., Cai, G., Guan, Z., Cai, S."Downregulation of S100A4 expression by RNA interference suppresses cell growth and invasion in human colorectal cancer cells". Oncology Reports 27, no. 4 (2012): 917-922. https://doi.org/10.3892/or.2011.1598
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