Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma

  • Authors:
    • Yuichi Ohnishi
    • Hiroshi Inoue
    • Masayuki Furukawa
    • Kenji Kakudo
    • Masami Nozaki
  • View Affiliations

  • Published online on: December 30, 2011     https://doi.org/10.3892/or.2011.1616
  • Pages: 954-958
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Abstract

Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) has been shown to stimulate the growth of various cell types in an autocrine or paracrine manner. Although HB-EGF is widely expressed in tumors when compared with normal tissue, its contribution to cancer progression remains obscure. The objective of this study was to explore the effects of HB-EGF on proliferation, invasion activity and MMP-9 levels of an oral squamous cell carcinoma cell line, HSC3, in vitro. MTT assays, Matrigel invasion assays and RT-PCR in combination with RNA interference (RNAi) were used in this study. An RNAi-mediated decrease in HB-EGF expression reduced invasion activity and MMP-9 mRNA levels, but not proliferation, in HSC3 cells. The addition of purified HB-EGF to cell culture medium upregulated MMP-9 mRNA levels in HSC3 cells. Furthermore, the TACE inhibitor TAPI-2 or EGFR inhibitor AG1478 decreased MMP-9 mRNA levels in HSC3 cells. These data indicate that HB-EGF released from HSC3 cells by TACE stimulates EGFR in an autocrine manner, which in turn activates invasion activity via MMP-9 upregulation.
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April 2012
Volume 27 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ohnishi Y, Inoue H, Furukawa M, Kakudo K and Nozaki M: Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma. Oncol Rep 27: 954-958, 2012.
APA
Ohnishi, Y., Inoue, H., Furukawa, M., Kakudo, K., & Nozaki, M. (2012). Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma. Oncology Reports, 27, 954-958. https://doi.org/10.3892/or.2011.1616
MLA
Ohnishi, Y., Inoue, H., Furukawa, M., Kakudo, K., Nozaki, M."Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma". Oncology Reports 27.4 (2012): 954-958.
Chicago
Ohnishi, Y., Inoue, H., Furukawa, M., Kakudo, K., Nozaki, M."Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma". Oncology Reports 27, no. 4 (2012): 954-958. https://doi.org/10.3892/or.2011.1616