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Article

AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice

  • Authors:
    • Sha-Sha He
    • Hua-Shan Shi
    • Tao Yin
    • Yong-Xia Li
    • Shun-Tao Luo
    • Qin-Jie Wu
    • Lian Lu
    • Yu-Quan Wei
    • Li Yang
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Keyuan Road 4, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1142-1148
    |
    Published online on: January 4, 2012
       https://doi.org/10.3892/or.2012.1621
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Abstract

Pigment epithelium-derived factor (PEDF) is the most potent inhibitor of angiogenesis in the mammalian eye, and mechanisms through which PEDF exerts its antitumour activity have recently been defined. The aim of our research was to evaluate the ability of adeno-associated virus (AAV) vector-mediated transfer of human PEDF to inhibit lewis lung carcinoma (LCC) cell growth. Intratumoural injection of AAV-PEDF caused significant reduction of the tumour volume and prolonged the survival time of mice bearing LLC cells, which were associated with decreased microvessel density and increased apoptosis in the tumours. AAV vectors represent a very promising tool for cancer gene therapy. No noticeable toxicity concerning AAV was detected as inferred from monitoring changes in animal body weight as well as basic organ structure and histological morphology, and by analyzing mouse liver and kidney function. Our findings indicate that AAV-mediated PEDF gene expression may offer an active approach to inhibit LLC growth and that treatment with AAV-PEDF may provide a promising therapeutic strategy in lung cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
He S, Shi H, Yin T, Li Y, Luo S, Wu Q, Lu L, Wei Y and Yang L: AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice. Oncol Rep 27: 1142-1148, 2012.
APA
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q. ... Yang, L. (2012). AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice. Oncology Reports, 27, 1142-1148. https://doi.org/10.3892/or.2012.1621
MLA
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q., Lu, L., Wei, Y., Yang, L."AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice". Oncology Reports 27.4 (2012): 1142-1148.
Chicago
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q., Lu, L., Wei, Y., Yang, L."AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice". Oncology Reports 27, no. 4 (2012): 1142-1148. https://doi.org/10.3892/or.2012.1621
Copy and paste a formatted citation
x
Spandidos Publications style
He S, Shi H, Yin T, Li Y, Luo S, Wu Q, Lu L, Wei Y and Yang L: AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice. Oncol Rep 27: 1142-1148, 2012.
APA
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q. ... Yang, L. (2012). AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice. Oncology Reports, 27, 1142-1148. https://doi.org/10.3892/or.2012.1621
MLA
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q., Lu, L., Wei, Y., Yang, L."AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice". Oncology Reports 27.4 (2012): 1142-1148.
Chicago
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q., Lu, L., Wei, Y., Yang, L."AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice". Oncology Reports 27, no. 4 (2012): 1142-1148. https://doi.org/10.3892/or.2012.1621
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