AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice

  • Authors:
    • Sha-Sha He
    • Hua-Shan Shi
    • Tao Yin
    • Yong-Xia Li
    • Shun-Tao Luo
    • Qin-Jie Wu
    • Lian Lu
    • Yu-Quan Wei
    • Li Yang
  • View Affiliations

  • Published online on: January 4, 2012     https://doi.org/10.3892/or.2012.1621
  • Pages: 1142-1148
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Abstract

Pigment epithelium-derived factor (PEDF) is the most potent inhibitor of angiogenesis in the mammalian eye, and mechanisms through which PEDF exerts its antitumour activity have recently been defined. The aim of our research was to evaluate the ability of adeno-associated virus (AAV) vector-mediated transfer of human PEDF to inhibit lewis lung carcinoma (LCC) cell growth. Intratumoural injection of AAV-PEDF caused significant reduction of the tumour volume and prolonged the survival time of mice bearing LLC cells, which were associated with decreased microvessel density and increased apoptosis in the tumours. AAV vectors represent a very promising tool for cancer gene therapy. No noticeable toxicity concerning AAV was detected as inferred from monitoring changes in animal body weight as well as basic organ structure and histological morphology, and by analyzing mouse liver and kidney function. Our findings indicate that AAV-mediated PEDF gene expression may offer an active approach to inhibit LLC growth and that treatment with AAV-PEDF may provide a promising therapeutic strategy in lung cancer treatment.
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April 2012
Volume 27 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
He S, Shi H, Yin T, Li Y, Luo S, Wu Q, Lu L, Wei Y and Yang L: AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice. Oncol Rep 27: 1142-1148, 2012
APA
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q. ... Yang, L. (2012). AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice. Oncology Reports, 27, 1142-1148. https://doi.org/10.3892/or.2012.1621
MLA
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q., Lu, L., Wei, Y., Yang, L."AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice". Oncology Reports 27.4 (2012): 1142-1148.
Chicago
He, S., Shi, H., Yin, T., Li, Y., Luo, S., Wu, Q., Lu, L., Wei, Y., Yang, L."AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice". Oncology Reports 27, no. 4 (2012): 1142-1148. https://doi.org/10.3892/or.2012.1621