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Article

Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells

  • Authors:
    • Bin-Chuan Ji
    • Chien-Chih Yu
    • Su-Tso Yang
    • Te-Chun Hsia
    • Jai-Sing Yang
    • Kuang-Chi Lai
    • Yang-Ching Ko
    • Jen-Jyh Lin
    • Tung-Yuan Lai
    • Jing-Gung Chung
  • View Affiliations / Copyright

    Affiliations: Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, Changhua 500, Taiwan, R.O.C., Department of Chinese Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, 91 Hsueh-Shih Road, Taichung 404, Taiwan, R.O.C.
  • Pages: 959-964
    |
    Published online on: January 4, 2012
       https://doi.org/10.3892/or.2012.1622
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Abstract

It has been shown that deguelin, one of the compounds of rotenoids from flavonoid family, induced cytotoxic effects through induction of cell cycle arrest and apoptosis in many types of human cancer cell lines, but deguelin-affected DNA damage and repair gene expression (mRNA) are not clarified yet. We investigated the effects of deguelin on DNA damage and associated gene expression in human lung cancer NCI-H460 cells in vitro. DNA damage was assayed by using the comet assay and DNA gel electrophoresis and the results indicated that NCI-H460 cells treated with 0, 50, 250 and 500 nM deguelin led to a longer DNA migration smear based on the single cell electrophoresis and DNA fragmentation occurred based on the examination of DNA gel electrophoresis. DNA damage and repair gene expression (mRNA) were evaluated by using real-time PCR assay and the results indicated that 50 and 250 nM deguelin for a 24-h exposure in NCI-H460 cells, decreased the gene levels of breast cancer 1, early onset (BRCA1), DNA-dependent serine/threonine protein kinase (DNA-PK), O6-methylguanine-DNA methyltransferase (MGMT), p53, ataxia telangiectasia mutated (ATM) and ataxia-telangiectasia and Rad3-related (ATR) mRNA expressions. Collectively, the present study showed that deguelin caused DNA damage and inhibited DNA damage and repair gene expressions, which might be due to deguelin-inhibited cell growth in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Ji B, Yu C, Yang S, Hsia T, Yang J, Lai K, Ko Y, Lin J, Lai T, Chung J, Chung J, et al: Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells. Oncol Rep 27: 959-964, 2012.
APA
Ji, B., Yu, C., Yang, S., Hsia, T., Yang, J., Lai, K. ... Chung, J. (2012). Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells. Oncology Reports, 27, 959-964. https://doi.org/10.3892/or.2012.1622
MLA
Ji, B., Yu, C., Yang, S., Hsia, T., Yang, J., Lai, K., Ko, Y., Lin, J., Lai, T., Chung, J."Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells". Oncology Reports 27.4 (2012): 959-964.
Chicago
Ji, B., Yu, C., Yang, S., Hsia, T., Yang, J., Lai, K., Ko, Y., Lin, J., Lai, T., Chung, J."Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells". Oncology Reports 27, no. 4 (2012): 959-964. https://doi.org/10.3892/or.2012.1622
Copy and paste a formatted citation
x
Spandidos Publications style
Ji B, Yu C, Yang S, Hsia T, Yang J, Lai K, Ko Y, Lin J, Lai T, Chung J, Chung J, et al: Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells. Oncol Rep 27: 959-964, 2012.
APA
Ji, B., Yu, C., Yang, S., Hsia, T., Yang, J., Lai, K. ... Chung, J. (2012). Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells. Oncology Reports, 27, 959-964. https://doi.org/10.3892/or.2012.1622
MLA
Ji, B., Yu, C., Yang, S., Hsia, T., Yang, J., Lai, K., Ko, Y., Lin, J., Lai, T., Chung, J."Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells". Oncology Reports 27.4 (2012): 959-964.
Chicago
Ji, B., Yu, C., Yang, S., Hsia, T., Yang, J., Lai, K., Ko, Y., Lin, J., Lai, T., Chung, J."Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells". Oncology Reports 27, no. 4 (2012): 959-964. https://doi.org/10.3892/or.2012.1622
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