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April 2012 Volume 27 Issue 4

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Article

Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells

  • Authors:
    • Ya-Hui Chen
    • Chi-Wei Yeh
    • Hui-Chen Lo
    • Shih-Li Su
    • You-Cheng Hseu
    • Li-Sung Hsu
  • View Affiliations / Copyright

    Affiliations: Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, R.O.C., Institute of Biochemistry and Biotechnology, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung 402, Taiwan, R.O.C.
  • Pages: 1233-1237
    |
    Published online on: January 12, 2012
       https://doi.org/10.3892/or.2012.1632
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Abstract

Flavonoids exhibit chemopreventive and chemotherapeutic effects. Butein, a bioactive flavonoid isolated from numerous native plants, has been shown to induce apoptosis in human cancer cells. In the current study, the molecular mechanisms of butein action on cell proliferation and apoptosis of neuroblastoma cells were evaluated. Treatment with butein decreased the viability of Neuro-2A neuroblastoma cells in a dose- and time-dependent manner. The dose-dependent nature of butein-induced apoptosis was characterized by an increase in the sub-G1 phase population. Treatment with butein significantly increased intracellular reactive oxygen species (ROS)levels and reduced the Bcl-2/Bax ratio, triggering the cleavage of pro-caspase 3 and poly-(ADP-ribose) polymerase (PARP). Pre-treatment with the antioxidant agent, N-acetyl cysteine (NAC), blocks butein-induced ROS generation and cell death. NAC also recovers butein-induced apoptosis-related protein alteration. In conclusion, butein-triggered neuroblastoma cells undergo apoptosis via generation of ROS, alteration of the Bcl‑2/Bax ratio, and cleavage of pro-caspase 3 and PARP. Our results suggest that butein may serve as a potential therapeutic agent for the treatment of neuroblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Yeh C, Lo H, Su S, Hseu Y and Hsu L: Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells. Oncol Rep 27: 1233-1237, 2012.
APA
Chen, Y., Yeh, C., Lo, H., Su, S., Hseu, Y., & Hsu, L. (2012). Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells. Oncology Reports, 27, 1233-1237. https://doi.org/10.3892/or.2012.1632
MLA
Chen, Y., Yeh, C., Lo, H., Su, S., Hseu, Y., Hsu, L."Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells". Oncology Reports 27.4 (2012): 1233-1237.
Chicago
Chen, Y., Yeh, C., Lo, H., Su, S., Hseu, Y., Hsu, L."Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells". Oncology Reports 27, no. 4 (2012): 1233-1237. https://doi.org/10.3892/or.2012.1632
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Yeh C, Lo H, Su S, Hseu Y and Hsu L: Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells. Oncol Rep 27: 1233-1237, 2012.
APA
Chen, Y., Yeh, C., Lo, H., Su, S., Hseu, Y., & Hsu, L. (2012). Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells. Oncology Reports, 27, 1233-1237. https://doi.org/10.3892/or.2012.1632
MLA
Chen, Y., Yeh, C., Lo, H., Su, S., Hseu, Y., Hsu, L."Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells". Oncology Reports 27.4 (2012): 1233-1237.
Chicago
Chen, Y., Yeh, C., Lo, H., Su, S., Hseu, Y., Hsu, L."Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells". Oncology Reports 27, no. 4 (2012): 1233-1237. https://doi.org/10.3892/or.2012.1632
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