Epithelial mesenchymal transition correlates with CD24+CD44+ and CD133+ cells in pancreatic cancer

  • Authors:
    • Ye Zhang
    • Jishu Wei
    • Hui Wang
    • Xiaofeng Xue
    • Yong An
    • Dong Tang
    • Zhongxu Yuan
    • Feitong Wang
    • Junli Wu
    • Jingjing Zhang
    • Yi Miao
  • View Affiliations

  • Published online on: February 7, 2012     https://doi.org/10.3892/or.2012.1681
  • Pages: 1599-1605
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Abstract

The epithelial-mesenchymal transition (EMT) has been linked to induction of a stem-cell like phenotype, characterized by altered cell surface marker expression and increased tumor formation. The aim of this study was to investigate whether EMT correlates with CD24+CD44+ and CD133+ cells in pancreatic cancer. The morphology of untreated and gemcitabine-treated SW1990 gemcitabine-resistant cells and normal SW1990 cells were compared. NF-κB p65 expression was knocked down using siRNA. Vimentin and E-cadherin expression were analyzed using western blotting, and CD24+CD44+, CD133+ cells were quantified by FACS. Additionally, immunohistochemistry of EMT-associated markers and stem cell-associated markers were performed in 41 cases of human pancreatic ductal adenocarcinoma. In SW1990 gemcitabine-resistant cells, gemcitabine induced a mesenchymal cell phenotype, expression of EMT-related molecular markers and increased CD24+CD44+ and CD133+ cells compared to untreated SW1990 gemcitabine-resistant and SW1990 cells. Knockdown of NF-κB p65 inhibited the ability of gemcitabine to increase the proportion of CD24+CD44+ or CD133+ cells and expression of EMT-related molecular markers. In human pancreatic ductal adenocarcinoma, significant correlations were observed between expression of the EMT-associated markers vimentin and E-cadherin, and stem cell-associated markers CD24, CD133 and CD44. This study demonstrated that EMT correlated with CD24+CD44+ and CD133+ cells in pancreatic cancer. This study also suggests that EMT may induce cancer stem-like cells in pancreatic cancer, with different degrees of EMT probability inducing different proportions of CD24+CD44+ and CD133+ cells.

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May 2012
Volume 27 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang Y, Wei J, Wang H, Xue X, An Y, Tang D, Yuan Z, Wang F, Wu J, Zhang J, Zhang J, et al: Epithelial mesenchymal transition correlates with CD24+CD44+ and CD133+ cells in pancreatic cancer. Oncol Rep 27: 1599-1605, 2012
APA
Zhang, Y., Wei, J., Wang, H., Xue, X., An, Y., Tang, D. ... Miao, Y. (2012). Epithelial mesenchymal transition correlates with CD24+CD44+ and CD133+ cells in pancreatic cancer. Oncology Reports, 27, 1599-1605. https://doi.org/10.3892/or.2012.1681
MLA
Zhang, Y., Wei, J., Wang, H., Xue, X., An, Y., Tang, D., Yuan, Z., Wang, F., Wu, J., Zhang, J., Miao, Y."Epithelial mesenchymal transition correlates with CD24+CD44+ and CD133+ cells in pancreatic cancer". Oncology Reports 27.5 (2012): 1599-1605.
Chicago
Zhang, Y., Wei, J., Wang, H., Xue, X., An, Y., Tang, D., Yuan, Z., Wang, F., Wu, J., Zhang, J., Miao, Y."Epithelial mesenchymal transition correlates with CD24+CD44+ and CD133+ cells in pancreatic cancer". Oncology Reports 27, no. 5 (2012): 1599-1605. https://doi.org/10.3892/or.2012.1681