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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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June 2012 Volume 27 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line

  • Authors:
    • Li-Li Liu
    • Li-Rong Lin
    • Man-Li Tong
    • Zuo-Gen Fu
    • Gui-Li Liu
    • Wei-Hong  Zheng
    • Fan Liu
    • Dai-Wei Zhang
    • Zhong-Ying Zhang
    • Tian-Ci Yang

  • View Affiliations / Copyright

    Affiliations: Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen University, Xiamen 361004, P.R. China, Department of Neurology, Zhongshan Hospital, Medical College of Xiamen University, Xiamen 361004, P.R. China, Medical College of Xiamen University, Xiamen 361004, P.R. China
  • Pages: 1829-1834
    |
    Published online on: February 28, 2012
       https://doi.org/10.3892/or.2012.1702
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Abstract

We constructed a vector carrying a shRNA sequence against cyclooxygenase-2 (COX-2) that was subsequently transfected into the human hepatocarcinoma cell line SMMC‑7721. Furthermore, we established a COX-2-deficient stable cell line and a model of tumor-shRNA transplantation in nude mice. Negative shRNA was used as the control. The tumor volume in the experimental group was smaller compared to that in the control group. Hematoxylin and eosin staining indicated that the cells in the experimental group differentiated better than those in the control group. The COX-2 mRNA level in the tumor tissues injected with SMMC-7721/COX-2i was markedly downregulated compared to that in the tumor tissues injected with SMMC-7721/negative shRNA. The inhibition rate reached 68.6%. Immunohistological study showed a significantly strong COX-2 expression in the control group tumor cells, whereas the experimental group exhibited moderate expression, indicating the inhibition of COX-2 expression after transfection of cells with shRNA against COX-2. Western blot analysis further proved the inhibition of COX-2 expression. In conclusion, RNAi-mediated regulation of COX-2 expression could efficiently inhibit liver-transplanted tumor growth in BALB/c nude mice.

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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Lin L, Tong M, Fu Z, Liu G, Zheng W, Liu F, Zhang D, Zhang Z, Yang T, Yang T, et al: Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line. Oncol Rep 27: 1829-1834, 2012.
APA
Liu, L., Lin, L., Tong, M., Fu, Z., Liu, G., Zheng, W. ... Yang, T. (2012). Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line. Oncology Reports, 27, 1829-1834. https://doi.org/10.3892/or.2012.1702
MLA
Liu, L., Lin, L., Tong, M., Fu, Z., Liu, G., Zheng, W., Liu, F., Zhang, D., Zhang, Z., Yang, T."Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line". Oncology Reports 27.6 (2012): 1829-1834.
Chicago
Liu, L., Lin, L., Tong, M., Fu, Z., Liu, G., Zheng, W., Liu, F., Zhang, D., Zhang, Z., Yang, T."Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line". Oncology Reports 27, no. 6 (2012): 1829-1834. https://doi.org/10.3892/or.2012.1702
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Lin L, Tong M, Fu Z, Liu G, Zheng W, Liu F, Zhang D, Zhang Z, Yang T, Yang T, et al: Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line. Oncol Rep 27: 1829-1834, 2012.
APA
Liu, L., Lin, L., Tong, M., Fu, Z., Liu, G., Zheng, W. ... Yang, T. (2012). Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line. Oncology Reports, 27, 1829-1834. https://doi.org/10.3892/or.2012.1702
MLA
Liu, L., Lin, L., Tong, M., Fu, Z., Liu, G., Zheng, W., Liu, F., Zhang, D., Zhang, Z., Yang, T."Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line". Oncology Reports 27.6 (2012): 1829-1834.
Chicago
Liu, L., Lin, L., Tong, M., Fu, Z., Liu, G., Zheng, W., Liu, F., Zhang, D., Zhang, Z., Yang, T."Effects of silencing cyclooxygenase-2 expression via RNA interference on the tumorigenicity of the SMMC-7721 human hepatocarcinoma cell line". Oncology Reports 27, no. 6 (2012): 1829-1834. https://doi.org/10.3892/or.2012.1702
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