Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest in HL-60 cells

  • Authors:
    • Lan Yi
    • Xiao-Xia Ji
    • Hui Tan
    • Mei-Yan Feng
    • Yi Tang
    • Ling Wen
    • Qi Su
  • View Affiliations

  • Published online on: February 28, 2012     https://doi.org/10.3892/or.2012.1704
  • Pages: 1911-1917
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Abstract

Diallyl disulfide (DADS) has shown potential as a therapeutic agent in various cancers. Previously, we found that myeloid cell leukemia sequence 1 (Mcl1) was downregulated in DADS-induced cell cycle arrest in HL-60 human leukemia cells. Here, we investigated the role of this protein in DADS-induced G2/M cell cycle arrest in HL-60 cells. We demonstrated that DADS treatment significantly increased the proportion of G2/M phase HL-60 cells (P<0.05) and caused a time-dependent significant downregulation of Mcl1 and the cell cycle-related proteins PCNA and CDK1 (P<0.05). Small interfering RNA-mediated knockdown of Mcl1 expression in HL-60 cells arrested the cell cycle in G2/M phase. By co-immunoprecipitation, we demonstrated that Mcl1 associated with PCNA and CDK1 in G2/M cell cycle arrest in DADS-treated HL-60 cells. DADS decreased the interaction of Mcl1 with PCNA and CDK1, leading to G2/M cell cycle arrest in HL-60 cells. Mcl1 plays an important role in DADS-induced G2/M cell cycle arrest in HL-60 human leukemia cells.

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June 2012
Volume 27 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yi L, Ji X, Tan H, Feng M, Tang Y, Wen L and Su Q: Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest in HL-60 cells. Oncol Rep 27: 1911-1917, 2012
APA
Yi, L., Ji, X., Tan, H., Feng, M., Tang, Y., Wen, L., & Su, Q. (2012). Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest in HL-60 cells. Oncology Reports, 27, 1911-1917. https://doi.org/10.3892/or.2012.1704
MLA
Yi, L., Ji, X., Tan, H., Feng, M., Tang, Y., Wen, L., Su, Q."Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest in HL-60 cells". Oncology Reports 27.6 (2012): 1911-1917.
Chicago
Yi, L., Ji, X., Tan, H., Feng, M., Tang, Y., Wen, L., Su, Q."Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest in HL-60 cells". Oncology Reports 27, no. 6 (2012): 1911-1917. https://doi.org/10.3892/or.2012.1704