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August 2012 Volume 28 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells

  • Authors:
    • Chang-Fu Qin
    • Kun Hao
    • Xiao-Dong Tian
    • Xue-Hai Xie
    • Yin-Mo Yang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Peking University First Hospital, Beijing 100034, P.R. China
  • Pages: 519-526
    |
    Published online on: May 9, 2012
       https://doi.org/10.3892/or.2012.1808
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Abstract

In the present study, we established a new experimental model to investigate the effects of EGFR targeting by RNAi, and the synergistic actions between the hedgehog (Hh) and EGFR signaling pathways on the proliferation and apoptosis in pancreatic cancer cells. Three human pancreatic cancer cell lines expressing EGFR shRNA were established, and gene expression inhibition was assessed in these lines using RT-PCR and western blot analysis. The effects of EGFR RNAi and Hh inhibition on cell proliferation and apoptosis were explored in vitro and in vivo. We observed that EGFR RNAi notably inhibited cell proliferation and colony formation, induced apoptosis and markedly decreased xenograft tumor growth. Furthermore, EGFR RNAi significantly enhanced cyclopamine sensitivity both in vitro and in vivo, and a synergistic decrease of both AKT and ERK phosphorylation was observed. The present study demonstrates that combined inhibition of both EGFR and Hh signaling pathways could establish a more promising antitumor approach than inhibiting each singly, and that there is a possible synergistic effect for Hh and EGFR signaling pathways on ERK and AKT phosphorylation.
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Copy and paste a formatted citation
Spandidos Publications style
Qin C, Hao K, Tian X, Xie X and Yang Y: Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells. Oncol Rep 28: 519-526, 2012.
APA
Qin, C., Hao, K., Tian, X., Xie, X., & Yang, Y. (2012). Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells. Oncology Reports, 28, 519-526. https://doi.org/10.3892/or.2012.1808
MLA
Qin, C., Hao, K., Tian, X., Xie, X., Yang, Y."Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells". Oncology Reports 28.2 (2012): 519-526.
Chicago
Qin, C., Hao, K., Tian, X., Xie, X., Yang, Y."Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells". Oncology Reports 28, no. 2 (2012): 519-526. https://doi.org/10.3892/or.2012.1808
Copy and paste a formatted citation
x
Spandidos Publications style
Qin C, Hao K, Tian X, Xie X and Yang Y: Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells. Oncol Rep 28: 519-526, 2012.
APA
Qin, C., Hao, K., Tian, X., Xie, X., & Yang, Y. (2012). Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells. Oncology Reports, 28, 519-526. https://doi.org/10.3892/or.2012.1808
MLA
Qin, C., Hao, K., Tian, X., Xie, X., Yang, Y."Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells". Oncology Reports 28.2 (2012): 519-526.
Chicago
Qin, C., Hao, K., Tian, X., Xie, X., Yang, Y."Combined effects of EGFR and Hedgehog signaling pathway inhibition on the proliferation and apoptosis of pancreatic cancer cells". Oncology Reports 28, no. 2 (2012): 519-526. https://doi.org/10.3892/or.2012.1808
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