Characterization of giant marker and ring chromosomes in a pleomorphic leiomyosarcoma of soft tissue by spectral karyotyping

  • Authors:
    • Jun Nishio
    • Mikiko Aoki
    • Kazuki Nabeshima
    • Hiroshi Iwasaki
    • Masatoshi Naito
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  • Published online on: May 25, 2012     https://doi.org/10.3892/or.2012.1835
  • Pages: 533-538
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Abstract

Pleomorphic leiomyosarcoma of soft tissue is relatively rare and its cytogenetic and molecular genetic data are scarce. We present a case of pleomorphic leiomyosarcoma arising in the left thigh of a 60-year-old man. Fluorine-18-deoxyglucose positron emission tomography imaging showed a homogenously high uptake within the mass in the proximal left thigh (maximum standardized uptake value, 20.9). Following a core needle biopsy, wide resection of the tumor was performed. Histologically, the tumor was composed of a mixture of spindle cells, polygonal cells and bizarre giant cells forming interlacing bundles and a storiform pattern. Immunohistochemically, the tumor cells were positive for vimentin, smooth muscle actin and desmin. The MIB-1 labeling index was 19.7% in the highest spot. Cytogenetic analysis exhibited a complex karyotype with several numerical and structural alterations, including giant marker and ring chromosomes. Spectral karyotyping demonstrated that giant marker and ring chromosomes were composed of material from the X chromosome. Metaphase-based comparative genomic hybridization analysis showed high-level amplifications of 1q21-q25 and 12q13-q21 and gains of 1p31-p32, 10p11-p13, 17p11 and 19p13. The patient received postoperative adjuvant radiotherapy and doxorubicin-based chemotherapy. No local recurrence or distant metastasis was detected during a follow-up period of 19 months. The clinicopathological, cytogenetic and molecular genetic features of pleomorphic soft tissue leiomyosarcoma are discussed.
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August 2012
Volume 28 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nishio J, Aoki M, Nabeshima K, Iwasaki H and Naito M: Characterization of giant marker and ring chromosomes in a pleomorphic leiomyosarcoma of soft tissue by spectral karyotyping. Oncol Rep 28: 533-538, 2012.
APA
Nishio, J., Aoki, M., Nabeshima, K., Iwasaki, H., & Naito, M. (2012). Characterization of giant marker and ring chromosomes in a pleomorphic leiomyosarcoma of soft tissue by spectral karyotyping. Oncology Reports, 28, 533-538. https://doi.org/10.3892/or.2012.1835
MLA
Nishio, J., Aoki, M., Nabeshima, K., Iwasaki, H., Naito, M."Characterization of giant marker and ring chromosomes in a pleomorphic leiomyosarcoma of soft tissue by spectral karyotyping". Oncology Reports 28.2 (2012): 533-538.
Chicago
Nishio, J., Aoki, M., Nabeshima, K., Iwasaki, H., Naito, M."Characterization of giant marker and ring chromosomes in a pleomorphic leiomyosarcoma of soft tissue by spectral karyotyping". Oncology Reports 28, no. 2 (2012): 533-538. https://doi.org/10.3892/or.2012.1835