Alterations of the base excision repair gene MUTYH in sporadic colorectal cancer

  • Authors:
    • Takashi Kuno
    • Nagahide Matsubara
    • Satoshi Tsuda
    • Masayoshi Kobayashi
    • Mie Hamanaka
    • Daisuke Yamagishi
    • Kiyoshi Tsukamoto
    • Tomoki Yamano
    • Masafumi Noda
    • Hiroki Ikeuchi
    • Sanghyuk Kim
    • Kazuo Tamura
    • Naohiro Tomita
  • View Affiliations

  • Published online on: May 25, 2012     https://doi.org/10.3892/or.2012.1836
  • Pages: 473-480
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Abstract

The base excision repair gene MUTYH encodes glycosylase which removes adenine residues mispaired with 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHG). Biallelic germline mutations of the MUTYH gene are known to cause multiple colorectal adenomas including polyposis and cancer, mostly due to G:C➝T:A transversions in proto-oncogenes or tumor suppressor genes. The risk of colorectal cancer (CRC) in monoallelic mutation carriers of MUTYH is estimated to be higher in comparison with non-carriers. To investigate the possible role in sporadic CRC, we examined alterations of the MUTYH gene including somatic mutations and allelic loss in 101 cases of sporadic CRC, together with the KRAS mutation in some cases. MUTYH mutations in cancer DNA were detected in 3 cases, while mutations were also found in DNA samples from normal tissues, indicating that all were germline mutations. Allelic loss at the MUTYH locus was found in 10 of 51 (20.0%) CRC cases and KRAS mutations were found in 33 of the 101 (32.7%) samples. There was no significant difference in the rate of G:C➝T:A transversion in KRAS between cases with allelic loss (1 of 10, 10.0%) and without allelic loss (9 of 41, 22.0%). Investigation of quantitative allelic imbalance at SNP rs3219489 of MUTYH showed that CRC cases with C allele dominance (minor type corresponding to His) were more frequently detected with G:C➝T:A transversions than in those with G allele dominance (major type corresponding to Gln). In conclusion, somatic alterations of MUTYH in sporadic CRC were rare, similar to other DNA repair genes. However, it is possible that unknown mutations of regions not analyzed in this study and epigenetic changes of the promoter region of MUTYH may contribute to the disease.

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August 2012
Volume 28 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Kuno, T., Matsubara, N., Tsuda, S., Kobayashi, M., Hamanaka, M., Yamagishi, D. ... Tomita, N. (2012). Alterations of the base excision repair gene MUTYH in sporadic colorectal cancer. Oncology Reports, 28, 473-480. https://doi.org/10.3892/or.2012.1836
MLA
Kuno, T., Matsubara, N., Tsuda, S., Kobayashi, M., Hamanaka, M., Yamagishi, D., Tsukamoto, K., Yamano, T., Noda, M., Ikeuchi, H., Kim, S., Tamura, K., Tomita, N."Alterations of the base excision repair gene MUTYH in sporadic colorectal cancer". Oncology Reports 28.2 (2012): 473-480.
Chicago
Kuno, T., Matsubara, N., Tsuda, S., Kobayashi, M., Hamanaka, M., Yamagishi, D., Tsukamoto, K., Yamano, T., Noda, M., Ikeuchi, H., Kim, S., Tamura, K., Tomita, N."Alterations of the base excision repair gene MUTYH in sporadic colorectal cancer". Oncology Reports 28, no. 2 (2012): 473-480. https://doi.org/10.3892/or.2012.1836