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Article

27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells

  • Authors:
    • Pamela Cruz
    • María José Epuñán
    • María Eugenia Ramírez
    • Cristian G. Torres
    • Luis E. Valladares
    • Walter D. Sierralta
  • View Affiliations / Copyright

    Affiliations: Laboratory of Nutrition and Metabolic Regulation, INTA-University of Chile, Santiago 7830489, Chile, Laboratory of Experimental Medicine, Veterinary Medicine School, University Andrés Bello, Santiago 8370251, Chile
  • Pages: 992-998
    |
    Published online on: June 12, 2012
       https://doi.org/10.3892/or.2012.1859
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Abstract

The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 µM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to the non-stimulated cells. Thus, the results confirm 27OHC's function as a novel selective estrogen receptor modulator (SERM). The epithelial to mesenchymal transition (EMT), observed in MCF7 cells stimulated for longer than 48 h with 2 µM 27OHC, was accompanied by lower immunoreactive levels of nuclear FOXM1 in comparison to E2-treated cells. The results presented in this study are discussed taking into consideration the relationship of hypercholesterolemia, 27OHC production, ROS synthesis and macrophage infiltration, potentially occurring in obese patients with ERα-positive, infiltrated mammary tumors.
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1 

Umetani M, Domoto H, Gormley AK, Yuhanna IS, Cummins CL, Javitt NB, Korach KS, Shaul PW and Mangelsdorf DJ: 27-Hydroxycholesterol is an endogenous SERM that inhibits the cardiovascular effects of estrogens. Nat Med. 13:1185–1192. 2007. View Article : Google Scholar : PubMed/NCBI

2 

DuSell CD, Umetani M, Shaul PW, Mangelsdorf DJ and McDonnell DP: 27-Hydroxycholesterol is an endogenous selective estrogen receptor modulator. Mol Endocrinol. 22:65–77. 2008. View Article : Google Scholar : PubMed/NCBI

3 

DuSell CD, Nelson ER, Wang X, Abdo J, Mödder UI, Umetani M, Gesty-Palmer D, Javitt NB, Khosla S and McDonnell DP: The endogenous selective estrogen receptor modulator 27-hydroxycholesterol is a negative regulator of bone homeostasis. Endocrinology. 151:3675–3685. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Nelson ER, DuSell CD, Wang X, Howe MK, Evans G, Michalek RD, Umetani M, Rathmell JC, Khosla S, Gesty-Palmer D and McDonnell DP: The oxysterol, 27-hydroxycholesterol links cholesterol metabolism to bone homeostasis through its actions on the estrogen and liver X receptors. Endocrinology. 152:4691–4705. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Cruz P, Torres C, Ramírez ME, Epuñán MI, Valladares LE and Sierralta WD: Proliferation of human mammary cancer cells exposed to 27-hydroxycholesterol. Exp Ther Med. 1:531–536. 2010.PubMed/NCBI

6 

Torres CG, Ramírez ME, Cruz P, Epuñan MI, Valladares LE and Sierralta WD: 27hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype. Oncol Rep. 26:389–397. 2011.PubMed/NCBI

7 

Zhou G, Dada LA, Wu M, Kelly A, Trejo H, Zhou Q, Varga J and Sznajder JI: Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondríal ROS and hypoxia-inducible factor I. Am J Physiol Lung Cell Mol Physiol. 297:L1120–L1130. 2009.PubMed/NCBI

8 

Boveris A and Chance B: The mitochondrial generation of hydrogen peroxide: general properties and effect of hyperbaric oxygen. Biochem J. 134:707–716. 1973.PubMed/NCBI

9 

Nemoto S, Takeda K, Yu Z-X, Ferrans VJ and Finkel T: Role for mitochondrial oxidants as regulators of cellular metabolism. Mol Cell Biol. 20:7311–7318. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Nicholls DG and Budd SU: Mitochondria and neuronal survival. Physiol Rev. 80:315–360. 2000.PubMed/NCBI

11 

Werner E and Werb Z: Integrins engage mitochondrial function for signal transduction by a mechanism dependent on Rho GTPases. J Cell Biol. 158:357–368. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Giannoni E, Bianchini F, Calorini L and Chiarugi P: Cancer associated fibroblasts exploit reactive oxygen species through a proinflammatory signature leading to epithelial mesenchymal transition and stemness. Antioxid Redox Signal. 14:2361–2371. 2011. View Article : Google Scholar

13 

Li Z, Shi K, Guan L, Cao T, Jiang Q, Yang Y and Xu C: ROS leads to MnSOD upregulation through ERK2 translocation and p53 activation in selenite-induced apoptosis of NB4 cells. FEBS Lett. 584:2291–2297. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Bakthavatchalu V, Dey S, Xu Y, Noel T, Jungsuwadee P, Holley AK, Dhar SK, Batinic-Haberle I and St Clair DK: Manganese superoxide dismutase is a mitochondrial fidelity protein that protects Polγ against UV-induced inactivation. Oncogene. Sep 12–2011.(Epub ahead of print). View Article : Google Scholar

15 

Chen M, Ni J, Chang HC, Lin CY, Muyan M and Yeh S: CCDC62/ERAP75 functions as a coactivator to enhance estrogen receptor beta-mediated transactivation and target gene expression in prostate cancer cells. Carcinogenesis. 30:841–850. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Monje P and Boland R: Subcellular distribution of native estrogen receptor alpha and beta isoforms in rabbit uterus and ovary. J Cell Biochem. 82:467–479. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Fox EM, Davis RJ and Shupnik MA: ERβ in breast cancer -onlooker, passive player or active protector? Steroids. 73:1039–1051. 2008.

18 

Yager ID and Chen JQ: Mitochondrial estrogen receptors-new insights into specific functions. Trends Endocrinol Metab. 18:89–91. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Klinge CM: Estrogenic control of mitochondrial function and biogenesis. J Cell Biochem. 105:1342–1351. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Psarra AM and Sekeris CE: Steroid and thyroid hormone receptors in mitochondria. IUBMB Life. 60:210–223. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Simpkins JW, Yang SH, Sarkar SN and Pearce V: Estrogen actions on mitochondria: physiological and pathological implications. Mol Cell Endocrinol. 290:51–59. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Hsieh YC, Yu HP, Suzuki T, Choudhry MA, Schwacha MG, Bland KI and Chaudry IH: Upregulation of mitochondrial respiratory complex IV by estrogen receptor-beta is critical for inhibiting mitochondrial apoptotic signaling and restoring cardiac functions following trauma-hemorrhage. J Mol Cell Cardiol. 41:511–521. 2006. View Article : Google Scholar

23 

Chen JQ, Russo PA, Cooke C, Russo IH and Russo J: ERbeta shifts from mitochondria to nucleus during estrogen-induced neoplastic transformation of human breast epithelial cells and is involved in estrogen-induced synthesis of mitochondrial respiratory chain proteins. Biochim Biophys Acta. 1773:1732–1746. 2007. View Article : Google Scholar

24 

Bakthavatchalu V, Dey S, Xu Y, Noel T, Jungsuwadee P, Holley AK, Dhar SK, BatinicHaberle I, St Chen JQ, Delannoy M, Cooke C and Yager ID: Mitochondrial localization of ERalpha and ERbeta in human MCF7 cells. Am J Physiol Endocrinol Metab. 286:E1011–E1022. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Madureira PA, Varshochi R, Constantinidou D, Francis RE, Coombes RC, Yao KM and Lam EW: The Forkhead box M1 protein regulates the transcription of the estrogen receptor alpha in breast cancer cells. J Biol Chem. 281:25167–25176. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Millour J, Constantinidou D, Stavropoulou AV, Wilson MS, Myatt SS, Kwok JM, Pedram A, Razandi M, Wallace DC and Levin ER: Functional estrogen receptors in the mitochondria of breast cancer cells. Mol Biol Cell. 17:2125–2137. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Francis RE, Myatt SS, Krol J, Hartman J, Peck B, McGovern UB, Wang J, Guest SK, Filipovic A, Gojis O, Palmieri C, Peston D, Shousha S, Yu Q, Sicinski P, Coombes RC and Lam EW: FoxM1 is a downstream target and marker of HER2 overexpression in breast cancer. Int J Oncol. 35:57–68. 2009.PubMed/NCBI

28 

Bektas N, Haaf A, Veeck J, Wild PI, Lüscher-Firzlaff J, Hartmann A, Knüchel R and Dahl E: Tight correlation between expression of the Forkhead transcription factor FOXMi and HER2 in human breast cancer. BMC Cancer. 8:422008. View Article : Google Scholar : PubMed/NCBI

29 

Sivanandan K, Coombes RC, Medema RH, Hartman J, Lykkesfeldt AE and Lam EW: FOXM1 is a transcriptional target of ERalpha and has a critical role in breast cancer endocrine sensitivity and resistance. Oncogene. 29:2983–2995. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Sierralta WD, Boenig I and Thole HH: Immunogold labelling of estradiol receptor in MCF 7 cells. Cell Tis Res. 279:445–452. 1995. View Article : Google Scholar : PubMed/NCBI

31 

Flohé L and Otting F: Superoxide dismutase assays. Methods Enzymol. 105:93–104. 1984.

32 

Powers SK, Lieu FK, Criswell D and Dodd S: Biochemical verification of quantitative histochemical analysis of succinate dehydrogenase activity in skeletal muscle fibres. Histochem J. 25:491–496. 1993. View Article : Google Scholar

33 

Giannoni E, Buricchi F, Grimaldi G, Parri M, Cialdai F, Taddei ML, Raugei G, Ramponi G and Chiarugi P: Redox regulation of anoikis: reactive oxygen species as essential mediators of cell survival. Cell Death Differ. 15:867–878. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Prasanthi JRP, Larson T, Schommer J and Ghribi O: Silencing GADD153/CHOP gene expression protects against Alzheimer’s disease-like pathology induced by 27-hydroxycholesterol in rabbit hippocampus. PLoS One. 6:e26420 View Article : Google Scholar : 2011.PubMed/NCBI

35 

Nilsson S and Gustafsson JA: Estrogen receptors: therapies targeted to receptor subtypes. Clin Pharmacol Ther. 89:44–55. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Zhao C, Dahlman-Wright K and Gustafsson JA: Estrogen signaling via estrogen receptors. J Biol Chem. 285:39575–39579. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Kwok JM, Peck B, Monteiro U, Schwenen HD, Millour J, Coombes RC, Myatt SS and Lam EW: FOXM1 confers acquired cisplatin resistance in breast cancer cells. Mol Cancer Res. 8:24–34. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Chua PJ, Yip GW and Bay BH: Cell cycle arrest induced by hydrogen peroxide is associated with modulation of oxidative stress related genes in breast cancer cells. Exp Biol Med. 234:1086–1094. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Karadedou CT: Regulation of the FOXM1 transcription factor by the estrogen receptor alpha at the protein level in breast cancer. Hippokratia. 10:128–132. 2006.PubMed/NCBI

40 

Zhang N, Wei P, Gong A, Chiu WT, Lee HT, Colman H, Huang H, Xue J, Liu M, Wang Y, Sawaya R, Xie K, Yung WK, Medema RH, He X and Huang S: FoxM1 promotes R-catenin nuclear localization and controls Wnt target-gene expression and glioma tumorigenesis. Cancer Cell. 20:427–442. 2011. View Article : Google Scholar : PubMed/NCBI

41 

Steele RJ, Eremin O, Brown M and Hawkins RA: A high macrophage content in human breast cancer is not associated with favourable prognostic factors. Br J Surg. 71:456–458. 1984. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cruz P, Epuñán MJ, Ramírez ME, Torres CG, Valladares LE and Sierralta WD: 27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells. Oncol Rep 28: 992-998, 2012.
APA
Cruz, P., Epuñán, M.J., Ramírez, M.E., Torres, C.G., Valladares, L.E., & Sierralta, W.D. (2012). 27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells. Oncology Reports, 28, 992-998. https://doi.org/10.3892/or.2012.1859
MLA
Cruz, P., Epuñán, M. J., Ramírez, M. E., Torres, C. G., Valladares, L. E., Sierralta, W. D."27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells". Oncology Reports 28.3 (2012): 992-998.
Chicago
Cruz, P., Epuñán, M. J., Ramírez, M. E., Torres, C. G., Valladares, L. E., Sierralta, W. D."27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells". Oncology Reports 28, no. 3 (2012): 992-998. https://doi.org/10.3892/or.2012.1859
Copy and paste a formatted citation
x
Spandidos Publications style
Cruz P, Epuñán MJ, Ramírez ME, Torres CG, Valladares LE and Sierralta WD: 27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells. Oncol Rep 28: 992-998, 2012.
APA
Cruz, P., Epuñán, M.J., Ramírez, M.E., Torres, C.G., Valladares, L.E., & Sierralta, W.D. (2012). 27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells. Oncology Reports, 28, 992-998. https://doi.org/10.3892/or.2012.1859
MLA
Cruz, P., Epuñán, M. J., Ramírez, M. E., Torres, C. G., Valladares, L. E., Sierralta, W. D."27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells". Oncology Reports 28.3 (2012): 992-998.
Chicago
Cruz, P., Epuñán, M. J., Ramírez, M. E., Torres, C. G., Valladares, L. E., Sierralta, W. D."27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells". Oncology Reports 28, no. 3 (2012): 992-998. https://doi.org/10.3892/or.2012.1859
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