27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells

  • Authors:
    • Pamela Cruz
    • María José Epuñán
    • María Eugenia Ramírez
    • Cristian G. Torres
    • Luis E. Valladares
    • Walter D. Sierralta
  • View Affiliations

  • Published online on: June 12, 2012     https://doi.org/10.3892/or.2012.1859
  • Pages: 992-998
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Abstract

The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 µM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to the non-stimulated cells. Thus, the results confirm 27OHC's function as a novel selective estrogen receptor modulator (SERM). The epithelial to mesenchymal transition (EMT), observed in MCF7 cells stimulated for longer than 48 h with 2 µM 27OHC, was accompanied by lower immunoreactive levels of nuclear FOXM1 in comparison to E2-treated cells. The results presented in this study are discussed taking into consideration the relationship of hypercholesterolemia, 27OHC production, ROS synthesis and macrophage infiltration, potentially occurring in obese patients with ERα-positive, infiltrated mammary tumors.
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September 2012
Volume 28 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cruz P, Epuñán MJ, Ramírez ME, Torres CG, Valladares LE and Sierralta WD: 27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells. Oncol Rep 28: 992-998, 2012
APA
Cruz, P., Epuñán, M.J., Ramírez, M.E., Torres, C.G., Valladares, L.E., & Sierralta, W.D. (2012). 27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells. Oncology Reports, 28, 992-998. https://doi.org/10.3892/or.2012.1859
MLA
Cruz, P., Epuñán, M. J., Ramírez, M. E., Torres, C. G., Valladares, L. E., Sierralta, W. D."27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells". Oncology Reports 28.3 (2012): 992-998.
Chicago
Cruz, P., Epuñán, M. J., Ramírez, M. E., Torres, C. G., Valladares, L. E., Sierralta, W. D."27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells". Oncology Reports 28, no. 3 (2012): 992-998. https://doi.org/10.3892/or.2012.1859