Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
September 2012 Volume 28 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 2012 Volume 28 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells

  • Authors:
    • Dong Li
    • Xiang-Pen Li
    • Hong-Xuan Wang
    • Qing-Yu Shen
    • Xiang-Ping Li
    • Lu Wen
    • Xiu-Jiao Qin
    • Qiu-Li Jia
    • Hsiang-Fu Kung
    • Ying Peng
  • View Affiliations / Copyright

    Affiliations: Laboratory of Integrated Biosciences, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, P.R. China, Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China, Department of ENT, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China
  • Pages: 937-942
    |
    Published online on: June 12, 2012
       https://doi.org/10.3892/or.2012.1861
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Zebrafish (Danio rerio) is becoming an increasingly popular vertebrate cancer model. In this study, we established a xenotransplanted zebrafish embryo glioma model to further investigate the molecular mechanisms of tumor angiogenesis. We find that the glioma cell line U87 can survive, proliferate and induce additional SIV branches in zebrafish embryos. In addition, by the means of in situ hybridization and quantitive RT-PCR analyses we find that the transplanted U87 cells can induce the ectopic zebrafish vascular endothelial growth factor A (VEGF A) and its receptor VEGFR2/KDR mRNA expression and increase their expression levels, resulting in additional SIV branches.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Ng SS, Gao Y, Chau DH, et al: A novel glioblastoma cancer gene therapy using AAV-mediated long-term expression of human TERT C-terminal polypeptide. Cancer Gene Ther. 14:561–572. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Lin ZX, Yang LJ, Huang Q, et al: Inhibition of tumor-induced edema by antisense VEGF is mediated by suppressive vesiculo-vacuolar organelles (VVO) formation. Cancer Sci. 99:2540–2546. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Paez-Ribes M, Allen E, Hudock J, et al: Antiangiogenic therapy elicits malignant progression of tumors to increased local invasion and distant metastasis. Cancer Cell. 15:220–231. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Norden AD, Drappatz J and Wen PY: Novel anti-angiogenic therapies for malignant gliomas. Lancet Neurol. 7:1152–1160. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Vredenburgh JJ, Desjardins A, Herndon JN, et al: Bevacizumab plus irinotecan in recurrent glioblastoma multiforme. J Clin Oncol. 25:4722–4729. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Tuettenberg J, Friedel C and Vajkoczy P: Angiogenesis in malignant glioma- a target for antitumor therapy? Crit Rev Oncol Hematol. 59:181–193. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Gan HK, Lappas M, Cao DX, Cvrljevdic A, Scott AM and Johns TG: Targeting a unique EGFR epitope with monoclonal antibody 806 activates NF-kappaB and initiates tumour vascular normalization. J Cell Mol Med. 13:3993–4001. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Jang FF, Wei W and De WM: Vascular endothelial growth factor and basic fibroblast growth factor expression positively correlates with angiogenesis and peritumoural brain oedema in astrocytoma. J Ayub Med Coll Abbottabad. 20:105–109. 2008.

9 

di Tomaso E, London N, Fuja D, et al: PDGF-C induces maturation of blood vessels in a model of glioblastoma and attenuates the response to anti-VEGF treatment. PLoS One. 4:e51232009.PubMed/NCBI

10 

Huse JT and Holland EC: Genetically engineered mouse models of brain cancer and the promise of preclinical testing. Brain Pathol. 19:132–143. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Lee LM, Seftor EA, Bonde G, Cornell RA and Hendrix MJ: The fate of human malignant melanoma cells transplanted into zebrafish embryos: assessment of migration and cell division in the absence of tumor formation. Dev Dyn. 233:1560–1570. 2005. View Article : Google Scholar

12 

Stoletov K, Montel V, Lester RD, Gonias SL and Klemke R: High-resolution imaging of the dynamic tumor cell vascular interface in transparent zebrafish. Proc Natl Acad Sci USA. 104:17406–17411. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Jesuthasan S: Genetics and development. Zebrafish in the spotlight. Science. 297:1484–1485. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Stoletov K and Klemke R: Catch of the day: zebrafish as a human cancer model. Oncogene. 27:4509–4520. 2008. View Article : Google Scholar : PubMed/NCBI

15 

White RM, Sessa A, Burke C, et al: Transparent adult zebrafish as a tool for in vivo transplantation analysis. Cell Stem Cell. 2:183–189. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Cross LM, Cook MA, Lin S, Chen JN and Rubinstein AL: Rapid analysis of angiogenesis drugs in a live fluorescent zebrafish assay. Arterioscler Thromb Vasc Biol. 23:911–912. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Westerfield M: The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio) Eugene, OR: University of Oregon Press; 2007

18 

Nicoli S, Ribatti D, Cotelli F and Presta M: Mammalian tumor xenografts induce neovascularization in zebrafish embryos. Cancer Res. 67:2927–2931. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Nicoli S and Presta M: The zebrafish/tumor xenograft angiogenesis assay. Nat Protoc. 2:2918–2923. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Serbedzija GN, Flynn E and Willett CE: Zebrafish angiogenesis: a new model for drug screening. Angiogenesis. 3:353–359. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Thisse C and Thisse B: High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat Protoc. 3:59–69. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Adhikary S and Eilers M: Transcriptional regulation and transformation by Myc proteins. Nat Rev Mol Cell Biol. 6:635–645. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Spitsbergen JM, Tsai HW, Reddy A, et al: Neoplasia in zebrafish (danio rerio) treated with 7,12-dimethylbenz[a]anthracene by two exposure routes at different developmental stages. Toxicol Pathol. 28:705–715. 2000.PubMed/NCBI

24 

Spitsbergen JM, Tsai HW, Reddy A, et al: Neoplasia in zebrafish (Danio rerio) treated with N-methyl-N′-nitro-N-nitrosoguanidine by three exposure routes at different developmental stages. Toxicol Pathol. 28:716–725. 2000.PubMed/NCBI

25 

Shepard JL, Amatruda JF, Stern HM, et al: A zebrafish bmyb mutation causes genome instability and increased cancer susceptibility. Proc Natl Acad Sci USA. 102:13194–13199. 2005.

26 

Amatruda JF, Shepard JL, Stern HM and Zon LI: Zebrafish as a cancer model system. Cancer Cell. 1:229–231. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Mione MC and Trede NS: The zebrafish as a model for cancer. Dis Model Mech. 3:517–523. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Chi A, Norden AD and Wen PY: Inhibition of angiogenesis and invasion in malignant gliomas. Expert Rev Anticancer Ther. 7:1537–1560. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Leung DW, Cachianes G, Kuang WJ, Goeddel DV and Ferrara N: Vascular endothelial growth factor is a secreted angiogenic mitogen. Science. 246:1306–1309. 1989. View Article : Google Scholar : PubMed/NCBI

30 

Plate KH, Breier G, Weich HA and Risau W: Vascular endothelial growth factor is a potential tumour angiogenesis factor in human gliomas in vivo. Nature. 359:845–848. 1992. View Article : Google Scholar : PubMed/NCBI

31 

Roy H, Bhardwaj S and Yla-Herttuala S: Biology of vascular endothelial growth factors. FEBS Lett. 580:2879–2887. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Neufeld G, Tessler S, Gitay-Goren H, Cohen T and Levi BZ: Vascular endothelial growth factor and its receptors. Prog Growth Factor Res. 5:89–97. 1994. View Article : Google Scholar : PubMed/NCBI

33 

Neufeld G, Cohen T, Gengrinovitch S and Poltorak Z: Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 13:9–22. 1999.PubMed/NCBI

34 

Habeck H, Odenthal J, Walderich B, Maischein H and Schulte-Merker S: Analysis of a zebrafish VEGF receptor mutant reveals specific disruption of angiogenesis. Curr Biol. 12:1405–1412. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Liang D, Xu X, Chin AJ, et al: Cloning and characterization of vascular endothelial growth factor (VEGF) from zebrafish, Danio rerio. Biochim Biophys Acta. 1397:14–20. 1998. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li D, Li X, Wang H, Shen Q, Li X, Wen L, Qin X, Jia Q, Kung H, Peng Y, Peng Y, et al: VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncol Rep 28: 937-942, 2012.
APA
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L. ... Peng, Y. (2012). VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncology Reports, 28, 937-942. https://doi.org/10.3892/or.2012.1861
MLA
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L., Qin, X., Jia, Q., Kung, H., Peng, Y."VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells". Oncology Reports 28.3 (2012): 937-942.
Chicago
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L., Qin, X., Jia, Q., Kung, H., Peng, Y."VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells". Oncology Reports 28, no. 3 (2012): 937-942. https://doi.org/10.3892/or.2012.1861
Copy and paste a formatted citation
x
Spandidos Publications style
Li D, Li X, Wang H, Shen Q, Li X, Wen L, Qin X, Jia Q, Kung H, Peng Y, Peng Y, et al: VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncol Rep 28: 937-942, 2012.
APA
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L. ... Peng, Y. (2012). VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncology Reports, 28, 937-942. https://doi.org/10.3892/or.2012.1861
MLA
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L., Qin, X., Jia, Q., Kung, H., Peng, Y."VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells". Oncology Reports 28.3 (2012): 937-942.
Chicago
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L., Qin, X., Jia, Q., Kung, H., Peng, Y."VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells". Oncology Reports 28, no. 3 (2012): 937-942. https://doi.org/10.3892/or.2012.1861
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team