VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells

  • Authors:
    • Dong Li
    • Xiang-Pen Li
    • Hong-Xuan Wang
    • Qing-Yu Shen
    • Xiang-Ping Li
    • Lu Wen
    • Xiu-Jiao Qin
    • Qiu-Li Jia
    • Hsiang-Fu Kung
    • Ying Peng
  • View Affiliations

  • Published online on: June 12, 2012     https://doi.org/10.3892/or.2012.1861
  • Pages: 937-942
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Abstract

Zebrafish (Danio rerio) is becoming an increasingly popular vertebrate cancer model. In this study, we established a xenotransplanted zebrafish embryo glioma model to further investigate the molecular mechanisms of tumor angiogenesis. We find that the glioma cell line U87 can survive, proliferate and induce additional SIV branches in zebrafish embryos. In addition, by the means of in situ hybridization and quantitive RT-PCR analyses we find that the transplanted U87 cells can induce the ectopic zebrafish vascular endothelial growth factor A (VEGF A) and its receptor VEGFR2/KDR mRNA expression and increase their expression levels, resulting in additional SIV branches.
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September 2012
Volume 28 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li D, Li X, Wang H, Shen Q, Li X, Wen L, Qin X, Jia Q, Kung H, Peng Y, Peng Y, et al: VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncol Rep 28: 937-942, 2012
APA
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L. ... Peng, Y. (2012). VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells. Oncology Reports, 28, 937-942. https://doi.org/10.3892/or.2012.1861
MLA
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L., Qin, X., Jia, Q., Kung, H., Peng, Y."VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells". Oncology Reports 28.3 (2012): 937-942.
Chicago
Li, D., Li, X., Wang, H., Shen, Q., Li, X., Wen, L., Qin, X., Jia, Q., Kung, H., Peng, Y."VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells". Oncology Reports 28, no. 3 (2012): 937-942. https://doi.org/10.3892/or.2012.1861