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Article

Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma

  • Authors:
    • Angelina Di Carlo
  • View Affiliations / Copyright

    Affiliations: Department of Medico-Surgical Sciences and Biotechnologies, ‘Sapienza’ University of Rome, I-00161 Rome, Italy
  • Pages: 1051-1056
    |
    Published online on: June 14, 2012
       https://doi.org/10.3892/or.2012.1864
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Abstract

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases, capable of degrading all the molecular components of extracellular matrix. MMPs have been shown to play critical roles in tumor cell invasion and metastasis. We verified the activity of MMPs in the sera and in the urine of patients with kidney carcinoma by gelatin zymography. Of these patients, 16 had clear cell renal carcinoma (ccRCC) and 4 patients had oncocytoma. The sera and the urine of 16 healthy subjects were used as controls. In the sera, zymography analysis showed gelatinolytic bands at 72 kDa (gelatinase A) at 92, 130 and 240 kDa (gelatinase B). MMP-9 activity was slightly enhanced in sera from ccRCC compared with oncocytoma patients. Serum MMP-2 activity was similar in ccRCC and in oncocytoma patients. In the urine, 2 oncocytoma patients and 3 (33%) of the ccRCC patients showed gelatinolytic activity, whereas MMPs could not be detected in the concentrated urine of healthy subjects. The most abundant lytic activity was at 92 kDa, whereas MMP-2 was present in lesser quantities. However, there was broad overlap of the data and we did not find any correlation to type, stage or grade. Therefore, despite previous evidence, MMP-2 and -9 activity in serum and urine may not be useful biomarker for kidney carcinomas.
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Copy and paste a formatted citation
Spandidos Publications style
Di Carlo A: Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma. Oncol Rep 28: 1051-1056, 2012.
APA
Di Carlo, A. (2012). Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma. Oncology Reports, 28, 1051-1056. https://doi.org/10.3892/or.2012.1864
MLA
Di Carlo, A."Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma". Oncology Reports 28.3 (2012): 1051-1056.
Chicago
Di Carlo, A."Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma". Oncology Reports 28, no. 3 (2012): 1051-1056. https://doi.org/10.3892/or.2012.1864
Copy and paste a formatted citation
x
Spandidos Publications style
Di Carlo A: Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma. Oncol Rep 28: 1051-1056, 2012.
APA
Di Carlo, A. (2012). Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma. Oncology Reports, 28, 1051-1056. https://doi.org/10.3892/or.2012.1864
MLA
Di Carlo, A."Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma". Oncology Reports 28.3 (2012): 1051-1056.
Chicago
Di Carlo, A."Matrix metalloproteinase-2 and -9 in the sera and in the urine of human oncocytoma and renal cell carcinoma". Oncology Reports 28, no. 3 (2012): 1051-1056. https://doi.org/10.3892/or.2012.1864
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