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September 2012 Volume 28 Issue 3

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Article

Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells

  • Authors:
    • Sho Tabata
    • Ryuji Ikeda
    • Masatatu Yamamoto
    • Tatsuhiko Furukawa
    • Takuya Kuramoto
    • Yasuo Takeda
    • Katsushi Yamada
    • Misako Haraguchi
    • Yasuhiko Nishioka
    • Saburo Sone
    • Shin-Ichi Akiyama
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan, Department of Clinical Pharmacy and Pharmacology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan, Department of Molecular Oncology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan, Department of Biochemistry and Molecular Biology, Graduate School of Medicine and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan, Department of Respiratory Medicine and Rheumatology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan
  • Pages: 895-902
    |
    Published online on: June 26, 2012
       https://doi.org/10.3892/or.2012.1887
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Abstract

Thymidine phosphorylase (TP) is an angiogenic factor that plays a pivotal role in tumor angiogenesis. Various kinds of solid tumors express TP and high TP activity is correlated with microvessel density. We have previously reported that TP enhances interleukin-8 (IL-8) expression in KB human epidermoid carcinoma cells. In this study, TP was shown to be involved in enhanced expression of IL-8 in EJ human bladder cancer cells and Yumoto human cervical cancer cells as well as KB human epidermoid carcinoma cells. The enzymatic activity of TP was required for the enhanced expression of IL-8. A degradation product of thymidine was implicated in the enhanced expression of IL-8. TP augmented reactive oxygen species (ROS) generation in KB and Yumoto cells, and the enzymatic activity of TP was again required for the generation of ROS. An antioxidant, N-acetylcysteine (NAC), attenuated the generation of ROS and IL-8 mRNA expression in KB and Yumoto cells, and H2O2 increased IL-8 mRNA expression in Yumoto cells, suggesting that ROS generated by TP caused the increased expression of IL-8 mRNA. Since TP also reduced cellular glutathione levels and transcription of γ-GCS in KB cells, the TP-induced augmentation of ROS may be partially attributed to the decreased glutathione. Our findings suggest that thymidine-derived sugars enhanced ROS generation and consequently increased IL-8 expression.
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1 

Furukawa T, Yoshimura A, Sumizawa T, et al: Angiogenic factor. Nature. 356:6681992.

2 

Akiyama S, Furukawa T, Sumizawa T, Takebayashi Y, Nakajima Y, Shimaoka S and Haraguchi M: The role of thymidine phosphorylase, an angiogenic enzyme, in tumor progression. Cancer Sci. 95:851–857. 2004.

3 

Miyadera K, Sumizawa T, Haraguchi M, Yoshida H, Konstanty W, Yamada Y and Akiyama S: Role of thymidine phosphorylase activity in the angiogenic effect of platelet derived endothelial cell growth factor/thymidine phosphorylase. Cancer Res. 55:1687–1690. 1995.

4 

Matsushita S, Nitanda T, Furukawa T, et al: The effect of a thymidine phosphorylase inhibitor on angiogenesis and apoptosis in tumors. Cancer Res. 59:1911–1916. 1999.

5 

Takao S, Akiyama S, Nakajo A, et al: Suppression of metastasis by thymidine phosphorylase inhibitor. Cancer Res. 60:5345–5348. 2000.

6 

Haraguchi M, Miyadera K, Uemura K, et al: Angiogenic activity of enzymes. Nature. 368:1981994.

7 

Kitazono M, Takebayashi Y, Ishitsuka K, et al: Prevention of hypoxia-induced apoptosis by the angiogenic factor, thymidine phosphprylase. Biochem Biophys Res Commun. 253:797–803. 1998.

8 

Uchimiya H, Furukawa T, Okamoto M, et al: Suppression of thymidine phosphorylase-mediated angiogenesis and tumor growth by 2-deoxy-l-ribose. Cancer Res. 62:2834–2839. 2002.

9 

Nakajima Y, Gotanda T, Uchimiya H, et al: Inhibition of metastasis of tumor cells overexpressing thymidine phosphorylase by 2-deoxy-l-ribose. Cancer Res. 64:1749–1801. 2004.

10 

Hotchkiss KA, Ashton AW and Schwartz EL: Thymidine phosphorylase and 2-deoxyribose stimulate human endothelial cell migration by specific activation of the integrins α5β1 and αVβ3. J Biol Chem. 278:19272–19279. 2003.

11 

Seeliger H, Guba M, Koehl GE, et al: Blockage of 2-deoxy-d-ribose induced angiogenesis with rapamycin counteracts a thymidine phosphorylase-based escape mechanism available for colon cancer under 5-fluorouracil therapy. Clin Cancer Res. 10:1843–1852. 2004.

12 

Hoffee PA: 2-Deoxyribose gene-enzyme complex in Salmonella typhimurium I. Isolation and enzymatic characterization of 2-deoxyribose-negative mutants. J Bacteriol. 95:449–457. 1968.

13 

Bijnsdorp IV, Azijli K, Jansen EE, et al: Accumulation of thymidine-derived sugars in thymidine phosphorylase overexpressing cells. Biochem Pharmacol. 80:786–792. 2010.

14 

Brown NS and Bicknell R: Thymidine phosphorylase, 2-deoxy-d-ribose and angiogenesis (Review). Biochem J. 334:1–8. 1988.

15 

Brown NS, Jones A, Fujiyama C, Harris AL and Bicknell R: Thymidine phosphorylase induces carcinoma cell oxidative stress and promotes secretion of angiogenic factors. Cancer Res. 60:6298–6302. 2000.

16 

Potter H, Weir L and Leder P: Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc Natl Acad Sci USA. 81:7161–7165. 1984.

17 

Takebayashi Y, Yamada K, Miyadera K, et al: The activity and expression of thymidine phosphorylase in human solid tumours. Eur J Cancer. 32A:1227–1232. 1996.

18 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970.

19 

Andersen JK: Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods. 10:203–209. 1984.

20 

Tuvdendorj D, Oketani M, Ikeda R, et al: Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway. Hepatol Res. 26:47–54. 2003.

21 

Fico A, Manganelli G, Cigliano L, et al: 2-Deoxy-d-ribose induces apoptosis by inhibiting the synthesis and increasing the efflux of glutathione. Free Radic Biol Med. 45:211–217. 2008.

22 

Schmidt MM, Greb H, Koliwer-Brandl H, Kelm S and Dringen R: 2-Deoxyribose deprives cultured astrocytes of their glutathione. Neurochem Res. 35:1848–1856. 2010.

23 

Brar SS, Kennedy TP, Sturrock AB, et al: NADPH oxidase promotes NF-κB activation and proliferation in human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 282:L782–L795. 2002.

24 

Murayama T, Ohara Y, Obuchi M, Khabar KSA, Higashi H, Mukaida N and Matsushima K: Human cytomegalovirus induces interleukin-8 production by a human monocytic cell line, THP-1, through acting concurrently on AP-1- and NF-κB-binding sites of the interleukin-8 gene. J Virol. 71:5692–5695. 1997.

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Copy and paste a formatted citation
Spandidos Publications style
Tabata S, Ikeda R, Yamamoto M, Furukawa T, Kuramoto T, Takeda Y, Yamada K, Haraguchi M, Nishioka Y, Sone S, Sone S, et al: Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells. Oncol Rep 28: 895-902, 2012.
APA
Tabata, S., Ikeda, R., Yamamoto, M., Furukawa, T., Kuramoto, T., Takeda, Y. ... Akiyama, S. (2012). Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells. Oncology Reports, 28, 895-902. https://doi.org/10.3892/or.2012.1887
MLA
Tabata, S., Ikeda, R., Yamamoto, M., Furukawa, T., Kuramoto, T., Takeda, Y., Yamada, K., Haraguchi, M., Nishioka, Y., Sone, S., Akiyama, S."Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells". Oncology Reports 28.3 (2012): 895-902.
Chicago
Tabata, S., Ikeda, R., Yamamoto, M., Furukawa, T., Kuramoto, T., Takeda, Y., Yamada, K., Haraguchi, M., Nishioka, Y., Sone, S., Akiyama, S."Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells". Oncology Reports 28, no. 3 (2012): 895-902. https://doi.org/10.3892/or.2012.1887
Copy and paste a formatted citation
x
Spandidos Publications style
Tabata S, Ikeda R, Yamamoto M, Furukawa T, Kuramoto T, Takeda Y, Yamada K, Haraguchi M, Nishioka Y, Sone S, Sone S, et al: Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells. Oncol Rep 28: 895-902, 2012.
APA
Tabata, S., Ikeda, R., Yamamoto, M., Furukawa, T., Kuramoto, T., Takeda, Y. ... Akiyama, S. (2012). Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells. Oncology Reports, 28, 895-902. https://doi.org/10.3892/or.2012.1887
MLA
Tabata, S., Ikeda, R., Yamamoto, M., Furukawa, T., Kuramoto, T., Takeda, Y., Yamada, K., Haraguchi, M., Nishioka, Y., Sone, S., Akiyama, S."Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells". Oncology Reports 28.3 (2012): 895-902.
Chicago
Tabata, S., Ikeda, R., Yamamoto, M., Furukawa, T., Kuramoto, T., Takeda, Y., Yamada, K., Haraguchi, M., Nishioka, Y., Sone, S., Akiyama, S."Thymidine phosphorylase enhances reactive oxygen species generation and interleukin-8 expression in human cancer cells". Oncology Reports 28, no. 3 (2012): 895-902. https://doi.org/10.3892/or.2012.1887
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