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September 2012 Volume 28 Issue 3

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Article

Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma

  • Authors:
    • Lanlan Zhou
    • Wafik El-Deiry
    • Wenge Wang
    • Mary E. Ingram
    • Sharyn I. Katz
  • View Affiliations / Copyright

    Affiliations: Department of Medicine (Hematology/Oncology), Laboratory of Translational Oncology and Experimental Cancer Therapeutics, Penn State College of Medicine, Penn State Hershey Cancer Institute, Penn State Hershey Medical Center, Hershey, PA, USA, Department of Internal Medicine, Mount Vernon Hospital, Mount Vernon, NY, USA, Department of Radiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA
  • Pages: 883-888
    |
    Published online on: June 26, 2012
       https://doi.org/10.3892/or.2012.1888
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Abstract

Malignant pleural mesothelioma (MPM) is locally aggressive and challenging to quantitate non-invasively in vivo, particularly in orthotopic models of disease. We describe imaging of extracellular protease activity, typically elevated in locally aggressive tumors, as a novel method for tracking MPM in vivo. Mice bearing human MPM subcutaneous flank xenografted tumors were imaged with ProSense 680, an optical imaging agent of extracellular cysteine protease activity. The relative contribution of extracellular cysteine proteases to the ProSense tumor signal was estimated using RT-PCR quantitation of cysteine protease RNA expression of the MPM cell lines and compared to ArrayExpress microarray RNA expression data from human MPM tumors. Feasibility of orthotopic intraperitoneal MPM cell mass tracking with fluorescence signal was evaluated using CellVue Maroon-coated MSTO-211H and compared to bioluminescent signal using luciferase-transfected MSTO-211H cells. ProSense 680 yielded a robust tumor signal in MPM subcutaneous grafts, primarily resulting from MPM secretion of cathepsin L demonstrated not only by RT-PCR data on MPM cell lines but also by microarray expression data from resected human patient tumors. CellVue Maroon intraperitoneal tumor signal was robust and durable indicating feasibility of intraperitoneal cell mass tracking of orthotopically-xenografted MPM. Optical imaging of extracellular cysteine protease activity is useful for tracking MPM tumor cell mass in vivo. Intraperitoneal MPM cell mass tracking of fluorescently labeled tumor is feasible.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou L, El-Deiry W, Wang W, Ingram ME and Katz S : Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma. Oncol Rep 28: 883-888, 2012.
APA
Zhou, L., El-Deiry, W., Wang, W., Ingram, M.E., & Katz, S. . (2012). Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma. Oncology Reports, 28, 883-888. https://doi.org/10.3892/or.2012.1888
MLA
Zhou, L., El-Deiry, W., Wang, W., Ingram, M. E., Katz, S. ."Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma". Oncology Reports 28.3 (2012): 883-888.
Chicago
Zhou, L., El-Deiry, W., Wang, W., Ingram, M. E., Katz, S. ."Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma". Oncology Reports 28, no. 3 (2012): 883-888. https://doi.org/10.3892/or.2012.1888
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou L, El-Deiry W, Wang W, Ingram ME and Katz S : Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma. Oncol Rep 28: 883-888, 2012.
APA
Zhou, L., El-Deiry, W., Wang, W., Ingram, M.E., & Katz, S. . (2012). Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma. Oncology Reports, 28, 883-888. https://doi.org/10.3892/or.2012.1888
MLA
Zhou, L., El-Deiry, W., Wang, W., Ingram, M. E., Katz, S. ."Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma". Oncology Reports 28.3 (2012): 883-888.
Chicago
Zhou, L., El-Deiry, W., Wang, W., Ingram, M. E., Katz, S. ."Extracellular protease imaging for cell mass tracking of xenografted human malignant pleural mesothelioma". Oncology Reports 28, no. 3 (2012): 883-888. https://doi.org/10.3892/or.2012.1888
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