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Article

Involvement of nuclear NHERF1 in colorectal cancer progression

  • Authors:
    • Anita Mangia
    • Concetta Saponaro
    • Andrea Malfettone
    • Domenico Bisceglie
    • Antonia Bellizzi
    • Mariaconsilia Asselti
    • Ondina Popescu
    • Stephan J. Reshkin
    • Angelo Paradiso
    • Giovanni Simone
  • View Affiliations / Copyright

    Affiliations: Functional Biomorphology Laboratory, National Cancer Centre, Bari, Italy, Department of Pathology, National Cancer Centre, Bari, Italy, Department of General and Environmental Physiology, University of Bari, Bari, Italy, Scientific Direction, National Cancer Centre, 70124 Bari, Italy
  • Pages: 889-894
    |
    Published online on: July 3, 2012
       https://doi.org/10.3892/or.2012.1895
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Abstract

NHERF1 (Na+/H+ exchanger regulatory factor 1) is expressed in the luminal membrane of many epithelia, and associated with proteins involved in tumor progression. Alterations of NHERF1 expression in different sites of metastatic colorectal cancer (mCRC) suggest a dynamic role of this protein in colon carcinogenesis. We focused on the observation of the altered expression of NHERF1 from non-neoplastic tissues to metastatic sites by immunohistochemistry. Moreover, we studied, by immunofluorescence, the colocalization between NHERF1 and the epidermal growth factor receptor (EGFR), whose overexpression is implicated in CRC progression. NHERF1 showed a different localization and expression in the examined sites. The distant non-neoplastic tissues showed NHERF1 mostly expressed at the apical membrane, while in surrounding non-neoplastic tissue decreased the apical membrane and increased cytoplasmic immunoreactivity. In adenomas a shift from apical membrane to cytoplasmic localization and nuclear expression were observed. Cytoplasmic staining in the tumor, and metastatic sites was stronger than surrounding non-neoplastic tissue. Furthermore, nuclear NHERF1 expression was noted in 80% of all samples and surprisingly, it appeared already in adenoma lesions, suggesting that NHERF1 represents an early marker of pre-morphological triggering of colorectal carcinogenesis. Then, in few tumors a positive direct correlation between membrane NHERF1 and EGFR expression was evidenced by their colocalization. Nuclear NHERF1 expression, present in the early stages of carcinogenesis and related with poor prognosis, may contribute to the onset of malignant phenotype. Specifically, we hypothesize the direct involvement of nuclear NHERF1 in both carcinogenesis and progression and its role as a potential colorectal cancer marker.
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Copy and paste a formatted citation
Spandidos Publications style
Mangia A, Saponaro C, Malfettone A, Bisceglie D, Bellizzi A, Asselti M, Popescu O, Reshkin SJ, Paradiso A, Simone G, Simone G, et al: Involvement of nuclear NHERF1 in colorectal cancer progression. Oncol Rep 28: 889-894, 2012.
APA
Mangia, A., Saponaro, C., Malfettone, A., Bisceglie, D., Bellizzi, A., Asselti, M. ... Simone, G. (2012). Involvement of nuclear NHERF1 in colorectal cancer progression. Oncology Reports, 28, 889-894. https://doi.org/10.3892/or.2012.1895
MLA
Mangia, A., Saponaro, C., Malfettone, A., Bisceglie, D., Bellizzi, A., Asselti, M., Popescu, O., Reshkin, S. J., Paradiso, A., Simone, G."Involvement of nuclear NHERF1 in colorectal cancer progression". Oncology Reports 28.3 (2012): 889-894.
Chicago
Mangia, A., Saponaro, C., Malfettone, A., Bisceglie, D., Bellizzi, A., Asselti, M., Popescu, O., Reshkin, S. J., Paradiso, A., Simone, G."Involvement of nuclear NHERF1 in colorectal cancer progression". Oncology Reports 28, no. 3 (2012): 889-894. https://doi.org/10.3892/or.2012.1895
Copy and paste a formatted citation
x
Spandidos Publications style
Mangia A, Saponaro C, Malfettone A, Bisceglie D, Bellizzi A, Asselti M, Popescu O, Reshkin SJ, Paradiso A, Simone G, Simone G, et al: Involvement of nuclear NHERF1 in colorectal cancer progression. Oncol Rep 28: 889-894, 2012.
APA
Mangia, A., Saponaro, C., Malfettone, A., Bisceglie, D., Bellizzi, A., Asselti, M. ... Simone, G. (2012). Involvement of nuclear NHERF1 in colorectal cancer progression. Oncology Reports, 28, 889-894. https://doi.org/10.3892/or.2012.1895
MLA
Mangia, A., Saponaro, C., Malfettone, A., Bisceglie, D., Bellizzi, A., Asselti, M., Popescu, O., Reshkin, S. J., Paradiso, A., Simone, G."Involvement of nuclear NHERF1 in colorectal cancer progression". Oncology Reports 28.3 (2012): 889-894.
Chicago
Mangia, A., Saponaro, C., Malfettone, A., Bisceglie, D., Bellizzi, A., Asselti, M., Popescu, O., Reshkin, S. J., Paradiso, A., Simone, G."Involvement of nuclear NHERF1 in colorectal cancer progression". Oncology Reports 28, no. 3 (2012): 889-894. https://doi.org/10.3892/or.2012.1895
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