The role of recombinant epidermal growth factor and serotonin in the stimulation of tumor growth in a SCCHN xenograft model

Geissler,Christin; Hambek,Markus; Eckardt,Anne; Arnoldner,Christoph; Diensthuber,Marc; Stöver,Timo; Wagenblast,Jens

doi:10.3892/or.2012.1903

September 2012 Volume 28 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

September 2012 Volume 28 Issue 3

Full Size Image

Article Open Access

The role of recombinant epidermal growth factor and serotonin in the stimulation of tumor growth in a SCCHN xenograft model

Authors:
- Christin Geissler
- Markus Hambek
- Anne Eckardt
- Christoph Arnoldner
- Marc Diensthuber
- Timo Stöver
- Jens Wagenblast
View Affiliations / Copyright

Affiliations: ENT Department, Medical School, Goethe University, Frankfurt am Main, Germany, ENT Department, Medical University of Vienna, Austria

Copyright: © Geissler et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 785-790
|
Published online on: July 6, 2012

https://doi.org/10.3892/or.2012.1903
Expand metrics +

Abstract

One challenge of squamous cell carcinoma of the head and neck (SCCHN) chemotherapy is a small percentage of tumor cells that arrest in the G0 phase of the cell cycle and are thus not affected by chemotherapy. This could be one reason for tumor recurrence at a later date. The recruitment of these G0-arresting cells into the active cell cycle and thus, proliferation, may increase the efficacy of chemotherapeutic agents. The aim of this study was to investigate whether stimulation with recombinant epidermal growth factor (EGF) or serotonin leads to an increased tumor cell proliferation in xenografts. Detroit 562 cells were injected into NMRI-Foxn1nu mice. Treatment was performed with 15 µg murine or human EGF, or 200 µg serotonin. The control mice were treated with Lactated Ringer's solution (5 mice/group). Tumor size was measured on days 4, 8 and 12 after tumor cell injection. The EGF stimulated mice showed a significantly higher tumor growth compared to the serotonin-stimulated mice and the untreated controls. In the present study, we show that it is possible to stimulate tumor cells in xenografts by EGF and thus, enhance cell proliferation, resulting in a higher tumor growth compared to the untreated control group. In our future investigations, we plan to include a higher number of mice, an adjustment of the EGF dosage and cell subanalysis, considering the heterogeneity of SCCHN tumors.

View Figures

View References

1	Matzinger O, Zouhair A, Mirimanoff RO and Ozsahin M: Radiochemotherapy in locally advanced squamous cell carcinomas of the head and neck. Clin Oncol (R Coll Radiol). 21:525–531. 2009. View Article : Google Scholar : PubMed/NCBI
2	Leemans CR, Braakhuis BJ and Brakenhoff RH: The molecular biology of head and neck cancer. Nat Rev Cancer. 11:9–22. 2011. View Article : Google Scholar
3	Robert Koch Institut. Krebs in Deutschland 2005/2006. Häufigkeiten und Trends. Westkreuz-Druckerei; Berlin: pp. 24–44. 2010
4	Argiris A, Karamouzis MV, Raben D and Ferris RL: Head and neck cancer. Lancet. 371:1695–1709. 2008. View Article : Google Scholar
5	Cohen EE: Role of epidermal growth factor receptor pathway-targeted therapy in patients with recurrent and/or metastatic squamous cell carcinoma of the head and neck. J Clin Oncol. 24:2659–2665. 2006. View Article : Google Scholar : PubMed/NCBI
6	Sharafinski ME, Ferris RL, Ferrone S and Grandis JR: Epidermal growth factor receptor targeted therapy of squamous cell carcinoma of the head and neck. Head Neck. 32:1412–1421. 2010. View Article : Google Scholar : PubMed/NCBI
7	Jost M, Kari C and Rodeck U: The EGF receptor - an essential regulator of multiple epidermal functions. Eur J Dermatol. 10:505–510. 2000.PubMed/NCBI
8	Vicaut E, Laemmel E and Stücker O: Impact of serotonin on tumour growth. Ann Med. 32:187–194. 2000. View Article : Google Scholar
9	Williams GH and Stoeber K: The cell cycle and cancer. J Pathol. 226:352–364. 2012. View Article : Google Scholar
10	Hambek M, Werner C, Baghi M, Gstöttner W and Knecht R: Enhancement of docetaxel efficacy in head and neck cancer treatment by G0 cell stimulation. Eur J Cancer. 43:1502–1507. 2007. View Article : Google Scholar : PubMed/NCBI
11	Kurago ZB, Lam-ubol A, Stone B and Stetsenko A: Cancer-supporting factors consistently induced by lipopolysaccharide - squamous cell carcinoma - monocyte interactions. Arch Otolaryngol Head Neck Surg. 132:9062006. View Article : Google Scholar
12	Bozec A, Lassalle S, Gugenheim J, Fischel JL, Formento P, Hofman P and Milano G: Enhanced tumour antiangiogenic effects when combining gefitinib with the antivascular agent ZD6126. Br J Cancer. 95:722–728. 2006. View Article : Google Scholar : PubMed/NCBI
13	Kalyankrishna S and Grandis JR: Epidermal growth factor receptor biology in head and neck cancer. J Clin Oncol. 24:2666–2672. 2006. View Article : Google Scholar : PubMed/NCBI
14	Ozawa S, Ueda M, Ando N, Abe O, Hirai M and Shimizu N: Stimulation by EGF of the growth of EGF receptor-hyperproducing tumor cells in athymic mice. Int J Cancer. 40:706–710. 1987. View Article : Google Scholar : PubMed/NCBI
15	Ginsburg E and Vonderhaar BK: Epidermal growth factor stimulates the growth of A431 tumors in athymic mice. Cancer Lett. 28:143–150. 1985. View Article : Google Scholar : PubMed/NCBI
16	Stern LE, Falcone RA Jr, Kemp CJ, Braun MC, Erwin CR and Warner BW: Salivary epidermal growth factor and intestinal adaptation in male and female mice. Am J Physiol Gastrointest Liver Physiol. 278:G871–G877. 2000.PubMed/NCBI
17	Schneider MR and Wolf E: The epidermal growth factor receptor ligands at a glance. J Cell Physiol. 218:460–466. 2009. View Article : Google Scholar : PubMed/NCBI
18	Pratesi G, Cervi S, Balsari A, Bondiolotti G and Vicentini LM: Effect of serotonin and nicotine on the growth of a human small cell lung cancer xenograft. Anticancer Res. 16:3615–3619. 1996.PubMed/NCBI
19	Shrivastav S, Joines WT and Jirtle RL: Effect of 5-hydroxytryptamine on tissue blood flow and microwave heating of rat tumors. Cancer Res. 45:3203–3208. 1985.PubMed/NCBI
20	Krishnamurthy S, Dong Z, Vodopyanov D, Imai A, Helman JI, Prince ME, Wicha MS and Nör JE: Endothelial cell-initiated signaling promotes the survival and self-renewal of cancer stem cells. Cancer Res. 70:9969–9978. 2010. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The role of recombinant epidermal growth factor and serotonin in the stimulation of tumor growth in a SCCHN xenograft model

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles