MicroRNA-9 suppresses uveal melanoma cell migration and invasion through the NF-κB1 pathway

Liu,Nannan; Sun,Qingmin; Chen,Jie; Li,Jiaqi; Zeng,Ying; Zhai,Sulan; Li,Ping; Wang,Bin; Wang,Xuerong

doi:10.3892/or.2012.1905

MicroRNA-9 suppresses uveal melanoma cell migration and invasion through the NF-κB1 pathway

Authors:
- Nannan Liu
- Qingmin Sun
- Jie Chen
- Jiaqi Li
- Ying Zeng
- Sulan Zhai
- Ping Li
- Bin Wang
- Xuerong Wang
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Affiliations: Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu Province, P.R. China, Department of Pharmacy, The Affiliated Wuxi Hospital for Maternal and Child Health Care of Nanjing Medical University, Wuxi, Jiangsu Province, P.R. China, Department of Pharmacy, The Affiliated Pingxiang Hospital, Southern Medical University, Pingxiang, Jiangxi Province, P.R. China
Published online on: July 6, 2012 https://doi.org/10.3892/or.2012.1905
Pages: 961-968

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Abstract

The aggressive course of uveal melanoma is believed to reflect its unusually invasive and metastatic nature, which is associated with the nuclear factor kappaB (NF-κB) pathway. MicroRNAs (miRNAs) have been implicated in the regulation of various biological and pathological processes in cancer, however, the special role of miR-9 in uveal melanoma metastasis is largely unknown. In the present study, we showed that miR-9 is significantly reduced in highly invasive uveal melanoma cell lines, and suppressed migration and invasion of highly invasive cells. Furthermore, miR-9 negatively modulated NF-κB1 expression by direct targeting at its 3'-UTRs. Additionally, downstream targets of NF-κB1, such as MMP-2, MMP-9 and VEGFA, were regulated by miR-9 in the same pattern as NF-κB1. Therefore, miR-9 suppresses uveal melanoma cell migration and invasion partly through downregulation of the NF-κB1 signaling pathway.

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