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October 2012 Volume 28 Issue 4

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Article

The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells

  • Authors:
    • Xueting Cai
    • Wuguang Lu
    • Tingmei Ye
    • Min Lu
    • Jianhua Wang
    • Jiege Huo
    • Shihui Qian
    • Xiaoning Wang
    • Peng Cao
  • View Affiliations / Copyright

    Affiliations: Laboratory of Cellular and Molecular Biology, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, P.R. China, Department of Biology, School of Chemistry and Life Sciences, Lishui University, Lishui 323000, P.R. China, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P.R. China
  • Pages: 1353-1361
    |
    Published online on: July 16, 2012
       https://doi.org/10.3892/or.2012.1914
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Abstract

Luteolin has been shown to have a strong anticancer effect on various cancer models via programmed cell death (apoptosis). However, the fundamental mechanisms of these effects are still unclear. In the present study, we examined the question of whether or not luteolin can inhibit proliferation of pancreatic carcinoma cells, via apoptosis. We used three human pancreatic carcinoma cell lines, PANC-1, CoLo‑357 and BxPC-3 in our study. In luteolin-treated pancreatic carcinoma cells, typical features of apoptosis were observed. Luteolin increased the expression of the pro-apoptotic protein Bax and decreased the expression of the anti-apoptotic protein Bcl-2, with a concomitant increase in the levels of caspase-3 and cleaved PARP after treatment for 24 h. Luteolin inhibited HUVEC proliferation and vessel growth in CAM in vivo. In addition, the concentration of VEGF in the conditioned medium from human pancreatic carcinoma cells was downregulated by luteolin. Pancreatic carcinoma cells, pretreated with luteolin, could decrease the capillary-like structure formation by HUVEC, which was analyzed by a co-culture system. The abatement of VEGF secretion was related to the inhibition of VEGF mRNA expression, which may be regulated by inhibiting the transcription activity of nuclear transcription factor NF-κB.
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Copy and paste a formatted citation
Spandidos Publications style
Cai X, Lu W, Ye T, Lu M, Wang J, Huo J, Qian S, Wang X and Cao P: The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells. Oncol Rep 28: 1353-1361, 2012.
APA
Cai, X., Lu, W., Ye, T., Lu, M., Wang, J., Huo, J. ... Cao, P. (2012). The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells. Oncology Reports, 28, 1353-1361. https://doi.org/10.3892/or.2012.1914
MLA
Cai, X., Lu, W., Ye, T., Lu, M., Wang, J., Huo, J., Qian, S., Wang, X., Cao, P."The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells". Oncology Reports 28.4 (2012): 1353-1361.
Chicago
Cai, X., Lu, W., Ye, T., Lu, M., Wang, J., Huo, J., Qian, S., Wang, X., Cao, P."The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells". Oncology Reports 28, no. 4 (2012): 1353-1361. https://doi.org/10.3892/or.2012.1914
Copy and paste a formatted citation
x
Spandidos Publications style
Cai X, Lu W, Ye T, Lu M, Wang J, Huo J, Qian S, Wang X and Cao P: The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells. Oncol Rep 28: 1353-1361, 2012.
APA
Cai, X., Lu, W., Ye, T., Lu, M., Wang, J., Huo, J. ... Cao, P. (2012). The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells. Oncology Reports, 28, 1353-1361. https://doi.org/10.3892/or.2012.1914
MLA
Cai, X., Lu, W., Ye, T., Lu, M., Wang, J., Huo, J., Qian, S., Wang, X., Cao, P."The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells". Oncology Reports 28.4 (2012): 1353-1361.
Chicago
Cai, X., Lu, W., Ye, T., Lu, M., Wang, J., Huo, J., Qian, S., Wang, X., Cao, P."The molecular mechanism of luteolin-induced apoptosis is potentially related to inhibition of angiogenesis in human pancreatic carcinoma cells". Oncology Reports 28, no. 4 (2012): 1353-1361. https://doi.org/10.3892/or.2012.1914
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