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Article

Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1

  • Authors:
    • Yinghai Zhao
    • Xiaoyi Chen
    • Min Jing
    • Haijun Du
    • Yi Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Guangdong Medical College, Zhanjiang 524023, P.R. China, Department of Pathology, the First People's Hospital of Nei Jiang, Neijiang 641000, P.R. China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 100052, P.R. China
  • Pages: 1237-1242
    |
    Published online on: July 25, 2012
       https://doi.org/10.3892/or.2012.1933
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Abstract

We aimed to investigate the relationship between miRNA-146a and latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC). The expression levels of LMP1 in 40 cases of NPC, 28 cases of chronic nasopharyngitis and NPC cell lines CNE1 and CNE1-GL (in which LMP1 was stably transfected) were detected by immunohistochemical staining. The expression of miRNA-146a in 16 cases of NPC, 13 cases of chronic nasopharyngitis and cell lines was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. A plasmid containing the luciferase gene under the control of miRNA-146a promoter (pri-miRNA-146a) was constructed and transfected into NPC cells, and the luciferase activity was detected. LMP1 was positive in 17.9% (5/28) of chronic nasopharyngitis cases and 62.5% (25/40) of NPC cases (p<0.01). The miRNA-146a levels in NPC were significantly higher than that in chronic nasopharyngitis (p<0.01), and were higher in CNE1-GL cells than those in CNE1 cells (p<0.01). The expression of miRNA-146a in human NPC was elevated by EBV-associated antigen LMP1, probably through the activation of the miRNA-146a promoter.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Chen X, Jing M, Du H and Zeng Y: Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1. Oncol Rep 28: 1237-1242, 2012.
APA
Zhao, Y., Chen, X., Jing, M., Du, H., & Zeng, Y. (2012). Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1. Oncology Reports, 28, 1237-1242. https://doi.org/10.3892/or.2012.1933
MLA
Zhao, Y., Chen, X., Jing, M., Du, H., Zeng, Y."Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1". Oncology Reports 28.4 (2012): 1237-1242.
Chicago
Zhao, Y., Chen, X., Jing, M., Du, H., Zeng, Y."Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1". Oncology Reports 28, no. 4 (2012): 1237-1242. https://doi.org/10.3892/or.2012.1933
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Chen X, Jing M, Du H and Zeng Y: Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1. Oncol Rep 28: 1237-1242, 2012.
APA
Zhao, Y., Chen, X., Jing, M., Du, H., & Zeng, Y. (2012). Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1. Oncology Reports, 28, 1237-1242. https://doi.org/10.3892/or.2012.1933
MLA
Zhao, Y., Chen, X., Jing, M., Du, H., Zeng, Y."Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1". Oncology Reports 28.4 (2012): 1237-1242.
Chicago
Zhao, Y., Chen, X., Jing, M., Du, H., Zeng, Y."Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1". Oncology Reports 28, no. 4 (2012): 1237-1242. https://doi.org/10.3892/or.2012.1933
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