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Article

Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1

  • Authors:
    • Wei Mao
    • Jie Chen
    • Tie-Li Peng
    • Xiao-Fei Yin
    • Lian-Zhou Chen
    • Min-Hu Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China, Tumor Tissue Bank, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, P.R. China
  • Pages: 1257-1262
    |
    Published online on: July 27, 2012
       https://doi.org/10.3892/or.2012.1939
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Abstract

Trefoil factor 1 (TFF1) is a small cysteine-rich secreted protein which is principally expressed in the superficial cells of gastric mucosa. In gastric cancer, TFF1 is downregulated and plays an important role. Gastrokine 1 (GKN1) is a secreted protein with similar expression and biological functions to TFF1. This study aimed to determine the expression and biological functions of TFF1 and the relationships between TFF1 and GKN1 in gastric cancer. RT-PCR and immunohistochemistry were performed to detect TFF1 expression in gastric cancer cell lines and tissues. The transfected and co-transfected AGS cells which stably expressed TFF1 or both TFF1 and GKN1 were generated. Phenotypic changes such as cell viability, apoptosis and cell cycle modulation were assayed in the transfected cells. We found that TFF1 expression was significantly downregulated or lost in gastric cancer cell lines, gastric dysplasia and cancer. Restoration of TFF1 expression in AGS cells suppressed tumor cell viability and arrested AGS cells in the G1-S transition phase after olomoucine treatment. However, TFF1 was unable to induce cell apoptosis. In co-transfected cells, we found that TFF1 and GKN1 did not directly interact at the protein level. GKN1 was unable to cooperate with TFF1 on cell viability suppression, cell apoptosis and differentiation. Together, these results indicate that TFF1 expression is significantly downregulated in gastric cancer. TFF1 inhibited cell proliferation by delaying G1-S phase transition but not by inducing apoptosis. TFF1 may not interact or cooperate with GKN1 at the protein and functional level.
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1 

Parkin DM, Bray FI and Devesa SS: Cancer burden in the year 2000. The global picture. Eur J Cancer. 37(Suppl 8): S4–S66. 2001.PubMed/NCBI

2 

Jemal A, Thomas A, Murray T and Thun M: Cancer statistics, 2002. CA Cancer J Clin. 52:23–47. 2002. View Article : Google Scholar

3 

Krejs GJ: Gastric cancer: epidemiology and risk factors. Dig Dis. 28:600–603. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Thim L: Trefoil peptides: from structure to function. Cell Mol Life Sci. 53:888–903. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Hoffmann W, Jagla W and Wiede A: Molecular medicine of TFF-peptides: from gut to brain. Histol Histopathol. 16:319–334. 2001.PubMed/NCBI

6 

Wright NA, Hoffmann W, Otto WR, Rio MC and Thim L: Rolling in the clover: trefoil factor family (TFF)-domain peptides, cell migration and cancer. FEBS Lett. 408:121–123. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Rio MC, Bellocq JP, Daniel JY, et al: Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa. Science. 241:705–708. 1988. View Article : Google Scholar : PubMed/NCBI

8 

Rio MC, Chenard MP, Wolf C, et al: Induction of pS2 and hSP genes as markers of mucosal ulceration of the digestive tract. Gastroenterology. 100:375–379. 1991.PubMed/NCBI

9 

Wright NA, Poulsom R, Stamp G, et al: Trefoil peptide gene expression in gastrointestinal epithelial cells in inflammatory bowel disease. Gastroenterology. 104:12–20. 1993.PubMed/NCBI

10 

Kjellev S: The trefoil factor family - small peptides with multiple functionalities. Cell Mol Life Sci. 66:1350–1369. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Henry JA, Bennett MK, Piggott NH, Levett DL, May FE and Westley BR: Expression of the pNR-2/pS2 protein in diverse human epithelial tumours. Br J Cancer. 64:677–682. 1991. View Article : Google Scholar : PubMed/NCBI

12 

Machado JC, Carneiro F, Blin N and Sobrinho-Simoes M: Pattern of pS2 protein expression in premalignant and malignant lesions of gastric mucosa. Eur J Cancer Prev. 5:169–179. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Lefebvre O, Chenard MP, Masson R, et al: Gastric mucosa abnormalities and tumorigenesis in mice lacking the pS2 trefoil protein. Science. 274:259–262. 1996. View Article : Google Scholar : PubMed/NCBI

14 

Calnan DP, Westley BR, May FE, Floyd DN, Marchbank T and Playford RJ: The trefoil peptide TFF1 inhibits the growth of the human gastric adenocarcinoma cell line AGS. J Pathol. 188:312–317. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Bossenmeyer-Pourie C, Kannan R, Ribieras S, et al: The trefoil factor 1 participates in gastrointestinal cell differentiation by delaying G1-S phase transition and reducing apoptosis. J Cell Biol. 157:761–770. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Prest SJ, May FE and Westley BR: The estrogen-regulated protein, TFF1, stimulates migration of human breast cancer cells. FASEB J. 16:592–594. 2002.PubMed/NCBI

17 

Ather MH, Abbas F, Faruqui N, Israr M and Pervez S: Expression of pS2 in prostate cancer correlates with grade and Chromogranin A expression but not with stage. BMC Urol. 4:142004. View Article : Google Scholar : PubMed/NCBI

18 

Arumugam T, Brandt W, Ramachandran V, et al: Trefoil factor 1 stimulates both pancreatic cancer and stellate cells and increases metastasis. Pancreas. 40:815–822. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Sanchez-Pulido L, Devos D and Valencia A: BRICHOS: a conserved domain in proteins associated with dementia, respiratory distress and cancer. Trends Biochem Sci. 27:329–332. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Yoshikawa Y, Mukai H, Hino F, Asada K and Kato I: Isolation of two novel genes, down-regulated in gastric cancer. Jpn J Cancer Res. 91:459–463. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Toback FG, Walsh-Reitz MM, Musch MW, et al: Peptide fragments of AMP-18, a novel secreted gastric antrum mucosal protein, are mitogenic and motogenic. Am J Physiol Gastrointest Liver Physiol. 285:G344–G353. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Walsh-Reitz MM, Huang EF, Musch MW, et al: AMP-18 protects barrier function of colonic epithelial cells: role of tight junction proteins. Am J Physiol Gastrointest Liver Physiol. 289:G163–G171. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Baus-Loncar M, Lubka M, Pusch CM, Otto WR, Poulsom R and Blin N: Cytokine regulation of the trefoil factor family binding protein GKN2 (GDDR/TFIZ1/blottin) in human gastrointestinal epithelial cells. Cell Physiol Biochem. 20:193–204. 2007.

24 

Westley BR, Griffin SM and May FE: Interaction between TFF1, a gastric tumor suppressor trefoil protein, and TFIZ1, a brichos domain-containing protein with homology to SP-C. Biochemistry. 44:7967–7975. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Otto WR and Thim L: Trefoil factor family-interacting proteins. Cell Mol Life Sci. 62:2939–2946. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Kouznetsova I, Laubinger W, Kalbacher H, et al: Biosynthesis of gastrokine-2 in the human gastric mucosa: restricted spatial expression along the antral gland axis and differential interaction with TFF1, TFF2 and mucins. Cell Physiol Biochem. 20:899–908. 2007. View Article : Google Scholar

27 

Stolte M and Meining A: The updated Sydney system: classification and grading of gastritis as the basis of diagnosis and treatment. Can J Gastroenterol. 15:591–598. 2001.PubMed/NCBI

28 

Beckler AD, Roche JK, Harper JC, et al: Decreased abundance of trefoil factor 1 transcript in the majority of gastric carcinomas. Cancer. 98:2184–2191. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Moss SF, Lee JW, Sabo E, et al: Decreased expression of gastrokine 1 and the trefoil factor interacting protein TFIZ1/GKN2 in gastric cancer: influence of tumor histology and relationship to prognosis. Clin Cancer Res. 14:4161–4167. 2008. View Article : Google Scholar : PubMed/NCBI

30 

May FE, Griffin SM and Westley BR: The trefoil factor interacting protein TFIZ1 binds the trefoil protein TFF1 preferentially in normal gastric mucosal cells but the co-expression of these proteins is deregulated in gastric cancer. Int J Biochem Cell Biol. 41:632–640. 2009. View Article : Google Scholar

31 

Rippa E, La Monica G, Allocca R, Romano MF, De Palma M and Arcari P: Overexpression of gastrokine 1 in gastric cancer cells induces fas-mediated apoptosis. J Cell Physiol. 226:2571–2578. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Mao W, Chen J, Peng T, Yin X, Chen L and Chen M: Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1. Oncol Rep 28: 1257-1262, 2012.
APA
Mao, W., Chen, J., Peng, T., Yin, X., Chen, L., & Chen, M. (2012). Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1. Oncology Reports, 28, 1257-1262. https://doi.org/10.3892/or.2012.1939
MLA
Mao, W., Chen, J., Peng, T., Yin, X., Chen, L., Chen, M."Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1". Oncology Reports 28.4 (2012): 1257-1262.
Chicago
Mao, W., Chen, J., Peng, T., Yin, X., Chen, L., Chen, M."Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1". Oncology Reports 28, no. 4 (2012): 1257-1262. https://doi.org/10.3892/or.2012.1939
Copy and paste a formatted citation
x
Spandidos Publications style
Mao W, Chen J, Peng T, Yin X, Chen L and Chen M: Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1. Oncol Rep 28: 1257-1262, 2012.
APA
Mao, W., Chen, J., Peng, T., Yin, X., Chen, L., & Chen, M. (2012). Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1. Oncology Reports, 28, 1257-1262. https://doi.org/10.3892/or.2012.1939
MLA
Mao, W., Chen, J., Peng, T., Yin, X., Chen, L., Chen, M."Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1". Oncology Reports 28.4 (2012): 1257-1262.
Chicago
Mao, W., Chen, J., Peng, T., Yin, X., Chen, L., Chen, M."Role of trefoil factor 1 in gastric cancer and relationship between trefoil factor 1 and gastrokine 1". Oncology Reports 28, no. 4 (2012): 1257-1262. https://doi.org/10.3892/or.2012.1939
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