The ubiquitin-conjugating enzyme E2-EPF is overexpressed in cervical cancer and associates with tumor growth

Liang,Jing; Nishi,Hirotaka; Bian,Mei-Lu; Higuma,Chinatsu; Sasaki,Toru; Ito,Hiroe; Isaka,Keiichi

doi:10.3892/or.2012.1949

October 2012 Volume 28 Issue 4

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October 2012 Volume 28 Issue 4

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Article

The ubiquitin-conjugating enzyme E2-EPF is overexpressed in cervical cancer and associates with tumor growth

Authors:
- Jing Liang
- Hirotaka Nishi
- Mei-Lu Bian
- Chinatsu Higuma
- Toru Sasaki
- Hiroe Ito
- Keiichi Isaka
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, China-Japan Friendship Hospital, Beijing, P.R. China, Department of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, Japan
Pages: 1519-1525
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Published online on: August 6, 2012

https://doi.org/10.3892/or.2012.1949
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Abstract

We found that the ubiquitin-conjugating enzyme E2-EPF mRNA is highly expressed in cervical squamous cancer relative to normal tissues and its expression levels positively correlate with clinical stage. Reduction of E2-EPF protein levels by >80% using shRNA decreases the expression levels of HIF-1α, and the proliferation, invasion and tumorigenicity of SiHa, a cervical squamous cancer cell line. E2-EPF knockdown also increases the chemosensitivity to topoisomerase I inhibitor (topotecan) and II (etoposide and doxorubicin). Our results suggest that E2-EPF is associated with the growth and aggressivity of cervical tumor cells. Targeting the E2-EPF pathway may have potential clinical applications for the treatment of cervical cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The ubiquitin-conjugating enzyme E2-EPF is overexpressed in cervical cancer and associates with tumor growth

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