Dioscin sensitizes cells to TRAIL-induced apoptosis through downregulation of c-FLIP and Bcl-2

Kim,Yong-Sik; Kim,Eun-Ae; Park,Kyu-Gun; Lee,Sung-Jun; Kim,Mi-Sun; Sohn,Ho-Yong; Lee,Tae-Jin

doi:10.3892/or.2012.1962

Dioscin sensitizes cells to TRAIL-induced apoptosis through downregulation of c-FLIP and Bcl-2

Authors:
- Yong-Sik Kim
- Eun-Ae Kim
- Kyu-Gun Park
- Sung-Jun Lee
- Mi-Sun Kim
- Ho-Yong Sohn
- Tae-Jin Lee
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Affiliations: Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan, Chungnam 330-090, Republic of Korea, Department of Anatomy, College of Medicine, Yeungnam University, 317-1 Daemyung-Dong Nam-Gu, Daegu 705‑717, Republic of Korea, Department of Food Science and Nutrition, Andong National University, Andong 760-749, Republic of Korea
Published online on: August 9, 2012 https://doi.org/10.3892/or.2012.1962
Pages: 1910-1916

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Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has received attention as a potential anticancer drug, because it induces apoptosis in a wide variety of cancer cells but not in most normal human cell types. Here, we showed that co-treatment with subtoxic doses of dioscin and TRAIL-induced apoptosis in Caki human renal cancer cells. Treatment of Caki cells with dioscin downregulated c-FLIPL and Bcl-2 proteins in a dose-dependent manner. Dioscin-induced decrease in c-FLIPL protein levels may be caused by the increased protein instability. We also found that dioscin induced downregulation of Bcl-2 at the transcriptional level. Pretreatment with NAC slightly inhibited the expression levels of c-FLIPL downregulated by the treatment of dioscin, suggesting that dioscin is partially dependent on the generation of ROS for downregulation of c-FLIPL. Taken together, the present study demonstrates that dioscin enhances TRAIL-induced apoptosis in human renal cancer cells by downregulation of c-FLIPL and Bcl-2.

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