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Article

Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer

  • Authors:
    • Yaning Chen
    • Chenfang Zhu
    • Zhiyou Peng
    • Yalei Dai
    • Yan Gu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, HuangPu District, Shanghai 200011, P.R. China, Department of Immunology, Shanghai Tongji University School of Medicine, YangPu District, Shanghai 200092, P.R. China
  • Pages: 1778-1784
    |
    Published online on: August 10, 2012
       https://doi.org/10.3892/or.2012.1964
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Abstract

In this study, we investigated the effects of lentivirus (LV)-mediated short hairpin RNA (shRNA) targeting IGF-1R on the growth and lymphangiogenesis of breast cancer. The LV vector effectively delivered the IGF-1R shRNA to MDA-MB‑231 cells, leading to significant reduction of IGF-1R mRNA and protein expression. Infection of MDA-MB-231 cells with LV-IGF-1R shRNA reduced cell growth and migration. Transplantation of MDA-MB-231 cells with suppressed IGF-1R expression in SCID mice reduced tumor growth and lymphangiogenesis. These data collectively suggest that LV-mediated shRNA is an effective way to suppress IGF-1R expression and to inhibit growth and lymphangiogenesis of breast cancer. Specific inhibition of IGF-1R expression with shRNA represents a promising approach for the treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Zhu C, Peng Z, Dai Y and Gu Y: Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer. Oncol Rep 28: 1778-1784, 2012.
APA
Chen, Y., Zhu, C., Peng, Z., Dai, Y., & Gu, Y. (2012). Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer. Oncology Reports, 28, 1778-1784. https://doi.org/10.3892/or.2012.1964
MLA
Chen, Y., Zhu, C., Peng, Z., Dai, Y., Gu, Y."Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer". Oncology Reports 28.5 (2012): 1778-1784.
Chicago
Chen, Y., Zhu, C., Peng, Z., Dai, Y., Gu, Y."Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer". Oncology Reports 28, no. 5 (2012): 1778-1784. https://doi.org/10.3892/or.2012.1964
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Zhu C, Peng Z, Dai Y and Gu Y: Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer. Oncol Rep 28: 1778-1784, 2012.
APA
Chen, Y., Zhu, C., Peng, Z., Dai, Y., & Gu, Y. (2012). Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer. Oncology Reports, 28, 1778-1784. https://doi.org/10.3892/or.2012.1964
MLA
Chen, Y., Zhu, C., Peng, Z., Dai, Y., Gu, Y."Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer". Oncology Reports 28.5 (2012): 1778-1784.
Chicago
Chen, Y., Zhu, C., Peng, Z., Dai, Y., Gu, Y."Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer". Oncology Reports 28, no. 5 (2012): 1778-1784. https://doi.org/10.3892/or.2012.1964
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