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Article

GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system

  • Authors:
    • Ge Zhang
    • Yan Liu
    • Liwei Yu
    • Lina Sun
  • View Affiliations / Copyright

    Affiliations: Beijing Haidian Hospital, Beijing 100080, P.R. China, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China
  • Pages: 1673-1680
    |
    Published online on: August 22, 2012
       https://doi.org/10.3892/or.2012.1976
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Abstract

Since mucosal high-risk human papillomavirus (HPV) E6 can target and degrade the tumor suppressor p53, it is recognized as a major causative agent of cervical cancer. However, to date the distribution of high-risk HPV-E6 protein remains elusive. Thus, in the present study we used a mammalian green fluorescent protein (GFP) expression system to express a GFP/HPV-16E6 fusion protein (GFP-16E6) in wild-type (wt) p53 cells, such as MCF-7 and 293T cells to investigate the trafficking and localization of E6 and p53. Following transfection, we observed that the overexpressed GFP-16E6 was a nuclear protein, and that endogenous wt p53 localized to the nucleus together with GFP-16E6. Strikingly, p53 levels were not decreased but increased in 24 h transfected with pGFP-16E6. Furthermore, we observed significant apoptosis induced by GFP-16E6, which proved to be dependent on p53 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang G, Liu Y, Yu L and Sun L: GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system. Oncol Rep 28: 1673-1680, 2012.
APA
Zhang, G., Liu, Y., Yu, L., & Sun, L. (2012). GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system. Oncology Reports, 28, 1673-1680. https://doi.org/10.3892/or.2012.1976
MLA
Zhang, G., Liu, Y., Yu, L., Sun, L."GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system". Oncology Reports 28.5 (2012): 1673-1680.
Chicago
Zhang, G., Liu, Y., Yu, L., Sun, L."GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system". Oncology Reports 28, no. 5 (2012): 1673-1680. https://doi.org/10.3892/or.2012.1976
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang G, Liu Y, Yu L and Sun L: GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system. Oncol Rep 28: 1673-1680, 2012.
APA
Zhang, G., Liu, Y., Yu, L., & Sun, L. (2012). GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system. Oncology Reports, 28, 1673-1680. https://doi.org/10.3892/or.2012.1976
MLA
Zhang, G., Liu, Y., Yu, L., Sun, L."GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system". Oncology Reports 28.5 (2012): 1673-1680.
Chicago
Zhang, G., Liu, Y., Yu, L., Sun, L."GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells using a transient expression system". Oncology Reports 28, no. 5 (2012): 1673-1680. https://doi.org/10.3892/or.2012.1976
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