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Article

Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells

  • Authors:
    • Haiquan Li
    • Keke Shao
    • Jingchun Wang
    • Guihua Wang
    • Jin Xu
    • Jinde Cao
    • Shaoqing Ju
    • Huimin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory Center, Affiliated Hospital of Nantong University, Nantong Medical College, Nantong University, Nantong 226001, P.R. China, Clinical Laboratory, Yancheng City Nο. 1 People's Hospital, Yancheng 224001, P.R. China
  • Pages: 1613-1618
    |
    Published online on: August 22, 2012
       https://doi.org/10.3892/or.2012.1980
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Abstract

A proliferation-inducing ligand (APRIL) is a key factor involved in the tumor development and progression in some tumor tissues and cells. Its overexpression and as gene target in SW480 colon carcinoma cells was confirmed in our previous study. To seek a more potent way to treat colon carcinoma using a gene therapy method, herein, we constructed a multiple short hairpin RNA (shRNA) expression vector containing four shRNAs against the APRIL gene in SW480 cells. APRIL expression levels and cell biological behavior were detected after transfection with different kinds of vectors. As expected, we found that our multiple shRNA vector produced a more significant knockdown effect of APRIL than the vectors containing only one APRIL shRNA. Furthermore, our findings indicate that silencing APRIL expression in SW480 cells decreased their malignancy by reducing proliferation, invasion and adhesion, as well as inducing apoptosis. Based on our findings, vectors containing multiple shRNAs to silence the expression of APRIL may be exploited as a novel therapeutic strategy for tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Shao K, Wang J, Wang G, Xu J, Cao J, Ju S and Wang H: Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells. Oncol Rep 28: 1613-1618, 2012.
APA
Li, H., Shao, K., Wang, J., Wang, G., Xu, J., Cao, J. ... Wang, H. (2012). Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells. Oncology Reports, 28, 1613-1618. https://doi.org/10.3892/or.2012.1980
MLA
Li, H., Shao, K., Wang, J., Wang, G., Xu, J., Cao, J., Ju, S., Wang, H."Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells". Oncology Reports 28.5 (2012): 1613-1618.
Chicago
Li, H., Shao, K., Wang, J., Wang, G., Xu, J., Cao, J., Ju, S., Wang, H."Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells". Oncology Reports 28, no. 5 (2012): 1613-1618. https://doi.org/10.3892/or.2012.1980
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Shao K, Wang J, Wang G, Xu J, Cao J, Ju S and Wang H: Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells. Oncol Rep 28: 1613-1618, 2012.
APA
Li, H., Shao, K., Wang, J., Wang, G., Xu, J., Cao, J. ... Wang, H. (2012). Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells. Oncology Reports, 28, 1613-1618. https://doi.org/10.3892/or.2012.1980
MLA
Li, H., Shao, K., Wang, J., Wang, G., Xu, J., Cao, J., Ju, S., Wang, H."Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells". Oncology Reports 28.5 (2012): 1613-1618.
Chicago
Li, H., Shao, K., Wang, J., Wang, G., Xu, J., Cao, J., Ju, S., Wang, H."Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells". Oncology Reports 28, no. 5 (2012): 1613-1618. https://doi.org/10.3892/or.2012.1980
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