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Article

Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors

  • Authors:
    • Dorota Zurawa-Janicka
    • Jarosław Kobiela
    • Natalia Galczynska
    • Tomasz Stefaniak
    • Barbara Lipinska
    • Andrzej Lachinski
    • Joanna Skorko-Glonek
    • Joanna Narkiewicz
    • Monika Proczko-Markuszewska
    • Zbigniew Sledzinski
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, University of Gdansk, 80-822 Gdansk, Poland, Department of General, Endocrine and Transplant Surgery, Medical University of Gdansk, 80-952 Gdansk, Poland
  • Pages: 1838-1844
    |
    Published online on: August 23, 2012
       https://doi.org/10.3892/or.2012.1988
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Abstract

Human HtrA proteins are serine proteases involved in essential physiological processes. HtrA1 and HtrA3 function as tumor suppressors and inhibitors of the TGF-β signaling pathway. HtrA2 regulates mitochondrial homeostasis and plays a pivotal role in the induction of apoptosis. The aim of the study was to determine whether the HtrA proteins are involved in thyroid carcinogenesis. We used the immunoblotting technique to estimate protein levels of HtrA1, HtrA2, long and short variants of HtrA3 (HtrA3-L and HtrA3-S) and TGF-β1 in tissues of benign and malignant thyroid lesions, and control groups. We found that the levels of HtrA2 and HtrA3-S were higher in thyroid malignant tumors compared to normal tissues and benign tumors. The HtrA3-L level was increased in malignant tumor tissues compared to benign tumor tissues and control tissues from patients with benign lesions, and elevated in normal tissues from patients with thyroid carcinoma compared to normal tissues from patients with benign lesions. We also compared levels of HtrA proteins in follicular thyroid carcinoma (FTC) and papillary thyroid carcinoma (PTC) and found that these types of carcinoma differed in the expression of HtrA3-S and HtrA1. These results indicate the implication of HtrA proteins in thyroid carcinogenesis suggest that HtrA3 variants may play different roles in cancer development, and that the increased HtrA3-L levels in thyroid tissue could be correlated with the development of malignant lesions. The TGF-β1 levels in tumor tissues were not significantly altered compared to control tissues.
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Copy and paste a formatted citation
Spandidos Publications style
Zurawa-Janicka D, Kobiela J, Galczynska N, Stefaniak T, Lipinska B, Lachinski A, Skorko-Glonek J, Narkiewicz J, Proczko-Markuszewska M, Sledzinski Z, Sledzinski Z, et al: Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors. Oncol Rep 28: 1838-1844, 2012.
APA
Zurawa-Janicka, D., Kobiela, J., Galczynska, N., Stefaniak, T., Lipinska, B., Lachinski, A. ... Sledzinski, Z. (2012). Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors. Oncology Reports, 28, 1838-1844. https://doi.org/10.3892/or.2012.1988
MLA
Zurawa-Janicka, D., Kobiela, J., Galczynska, N., Stefaniak, T., Lipinska, B., Lachinski, A., Skorko-Glonek, J., Narkiewicz, J., Proczko-Markuszewska, M., Sledzinski, Z."Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors". Oncology Reports 28.5 (2012): 1838-1844.
Chicago
Zurawa-Janicka, D., Kobiela, J., Galczynska, N., Stefaniak, T., Lipinska, B., Lachinski, A., Skorko-Glonek, J., Narkiewicz, J., Proczko-Markuszewska, M., Sledzinski, Z."Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors". Oncology Reports 28, no. 5 (2012): 1838-1844. https://doi.org/10.3892/or.2012.1988
Copy and paste a formatted citation
x
Spandidos Publications style
Zurawa-Janicka D, Kobiela J, Galczynska N, Stefaniak T, Lipinska B, Lachinski A, Skorko-Glonek J, Narkiewicz J, Proczko-Markuszewska M, Sledzinski Z, Sledzinski Z, et al: Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors. Oncol Rep 28: 1838-1844, 2012.
APA
Zurawa-Janicka, D., Kobiela, J., Galczynska, N., Stefaniak, T., Lipinska, B., Lachinski, A. ... Sledzinski, Z. (2012). Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors. Oncology Reports, 28, 1838-1844. https://doi.org/10.3892/or.2012.1988
MLA
Zurawa-Janicka, D., Kobiela, J., Galczynska, N., Stefaniak, T., Lipinska, B., Lachinski, A., Skorko-Glonek, J., Narkiewicz, J., Proczko-Markuszewska, M., Sledzinski, Z."Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors". Oncology Reports 28.5 (2012): 1838-1844.
Chicago
Zurawa-Janicka, D., Kobiela, J., Galczynska, N., Stefaniak, T., Lipinska, B., Lachinski, A., Skorko-Glonek, J., Narkiewicz, J., Proczko-Markuszewska, M., Sledzinski, Z."Changes in expression of human serine protease HtrA1, HtrA2 and HtrA3 genes in benign and malignant thyroid tumors". Oncology Reports 28, no. 5 (2012): 1838-1844. https://doi.org/10.3892/or.2012.1988
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