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Article

Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2

  • Authors:
    • Chia-Ming Chang
    • Pei-Ying Chang
    • Ming-Gene Tu
    • Chi-Cheng Lu
    • Sheng-Chu Kuo
    • Sakae Amagaya
    • Chao-Ying Lee
    • Hui-Yu Jao
    • Michael Yuanchien Chen
    • Jai-Sing Yang
  • View Affiliations / Copyright

    Affiliations: Department of Dentistry, China Medical University, Taichung, Taiwan, R.O.C., Department of Oral Maxillofacial Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C., Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, R.O.C., Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung, Taiwan, R.O.C., Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University, Saitama, Japan, School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C., Department of Pharmacology, China Medical University, Taichung, Taiwan, R.O.C.
  • Pages: 1799-1807
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    Published online on: August 24, 2012
       https://doi.org/10.3892/or.2012.1991
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Abstract

Human head and neck squamous cell carcinoma (HNSCC) is a major cause of cancer-related death during the last decade due to its related metastasis and poor treatment outcomes. Gefitinib (Iressa), a tyrosine kinase inhibitor has been reported to reduce the metastatic abilities of oral cancer. Previous studies have shown that epigallocatechin gallate (EGCG), a green tea polyphenol, possesses cancer chemopreventive and anticancer activity. However, the mechanisms involved in the suppression of invasion and metastasis of human oral cancer cells following co-incubation with gefitinib and EGCG remain poorly understood. In the present study, we attempted to investigate the synergistic effects of a combined treatment of gefitinib and EGCG in CAL-27 cells in vitro and to elucidate the underlying molecular mechanisms associated with the supression of cell migration and invasion. In the present study, we found that the individual treatments or the combined treatment of gefitinib and EGCG synergistically inhibited the invasion and migration of CAL-27 cells using Transwell invasion and wound-healing scratch assays, respectively. Similarly, gefitinib in combination with EGCG synergistically attenuated enzymatic activity and the protein expression of MMP-2 in CAL-27 cells. Furthermore, individual or combined treatment with EGCG and gefitinib suppressed the protein expression of p-EGFR and the phosphorylated protein levels of ERK, JNK, p38 and AKT and displayed inhibitory effects on metastatic ability of CAL-27 cells. Combined effects of EGCG and gefitinib-altered anti-metastatic actions for related gene expression were observed using DNA microarray analysis. Importantly, EGCG sensitizes CAL-27 cells to gefitinib-suppressed phosphorylation of epidermal growth factor receptor (EGFR in vitro. Taken together, our results suggest that the synergistic suppression of the metastatic ability of CAL-27 cells after EGCG and gefitinib individual or combined treatment are mediated through mitogen-activated protein kinase (MAPK) signaling. Our novel findings provide potential insights into the mechanism involved with synergistic responses of gefitinib and EGCG against the progression of oral cancer.
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1 

Liu SY, Lu CL, Chiou CT, et al: Surgical outcomes and prognostic factors of oral cancer associated with betel quid chewing and tobacco smoking in Taiwan. Oral Oncol. 46:276–282. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Chen PT, Kuan FC, Huang CE, et al: Incidence and patterns of second primary malignancies following oral cavity cancers in a prevalent area of betel-nut chewing: a population-based cohort of 26,166 patients in Taiwan. Jpn J Clin Oncol. 41:1336–1343. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Wang SC, Tsai CC, Huang ST and Hong YJ: Betel nut chewing and related factors in adolescent students in Taiwan. Public Health. 117:339–345. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Yu FS, Yang JS, Yu CS, et al: Safrole induces apoptosis in human oral cancer HSC-3 cells. J Dent Res. 90:168–174. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Choi Y, Kim SY, Kim SH, Yang J, Park K and Byun Y: Inhibition of tumor growth by biodegradable microspheres containing all-trans-retinoic acid in a human head-and-neck cancer xenograft. Int J Cancer. 107:145–148. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Muir C and Weiland L: Upper aerodigestive tract cancers. Cancer. 75:147–153. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Funk GF, Karnell LH, Robinson RA, Zhen WK, Trask DK and Hoffman HT: Presentation, treatment, and outcome of oral cavity cancer: a National Cancer Data Base report. Head Neck. 24:165–180. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Sommer G, Rossa C, Chi AC, Neville BW and Heise T: Implication of RNA-binding protein La in proliferation, migration and invasion of lymph node-metastasized hypopharyngeal SCC cells. PLoS One. 6:e254022011. View Article : Google Scholar : PubMed/NCBI

9 

Lu Z, Lu N, Li C, et al: Oroxylin A inhibits matrix metalloproteinase-2/9 expression and activation by up-regulating tissue inhibitor of metalloproteinase-2 and suppressing the ERK1/2 signaling pathway. Toxicol Lett. 209:211–220. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Ni L, Feng Y, Wan H, et al: Angiotensin-(1–7) inhibits the migration and invasion of A549 human lung adenocarcinoma cells through inactivation of the PI3K/Akt and MAPK signaling pathways. Oncol Rep. 27:783–790. 2012.

11 

Kim SA, Kwon SM, Kim JA, Kang KW, Yoon JH and Ahn SG: 5′-Nitro-indirubinoxime, an indirubin derivative, suppresses metastatic ability of human head and neck cancer cells through the inhibition of Integrin beta1/FAK/Akt signaling. Cancer Lett. 306:197–204. 2011.

12 

Liang X, Yang X, Tang Y, et al: RNAi-mediated downregulation of urokinase plasminogen activator receptor inhibits proliferation, adhesion, migration and invasion in oral cancer cells. Oral Oncol. 44:1172–1180. 2008. View Article : Google Scholar

13 

Ono M and Kuwano M: Molecular mechanisms of epidermal growth factor receptor (EGFR) activation and response to gefitinib and other EGFR-targeting drugs. Clin Cancer Res. 12:7242–7251. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Normanno N, De Luca A, Bianco C, et al: Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 366:2–16. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Schuler PJ, Boeckers P, Engers R, et al: EGFR-specific T cell frequencies correlate with EGFR expression in head and neck squamous cell carcinoma. J Transl Med. 9:1682011. View Article : Google Scholar : PubMed/NCBI

16 

Chiang WF, Liu SY, Yen CY, et al: Association of epidermal growth factor receptor (EGFR) gene copy number amplification with neck lymph node metastasis in areca-associated oral carcinomas. Oral Oncol. 44:270–276. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Korner A, Mudduluru G, Manegold C and Allgayer H: Enzastaurin inhibits invasion and metastasis in lung cancer by diverse molecules. Br J Cancer. 103:802–811. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Matsuo M, Sakurai H and Saiki I: ZD1839, a selective epidermal growth factor receptor tyrosine kinase inhibitor, shows antimetastatic activity using a hepatocellular carcinoma model. Mol Cancer Ther. 2:557–561. 2003.

19 

Yamaoka T, Frey MR, Dise RS, Bernard JK and Polk DB: Specific epidermal growth factor receptor autophosphorylation sites promote mouse colon epithelial cell chemotaxis and restitution. Am J Physiol Gastrointest Liver Physiol. 301:G368–G376. 2011. View Article : Google Scholar

20 

Hwang YP, Yun HJ, Choi JH, et al: Suppression of EGF-induced tumor cell migration and matrix metalloproteinase-9 expression by capsaicin via the inhibition of EGFR-mediated FAK/Akt, PKC/Raf/ERK, p38 MAPK, and AP-1 signaling. Mol Nutr Food Res. 55:594–605. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Rebucci M, Peixoto P, Dewitte A, et al: Mechanisms underlying resistance to cetuximab in the HNSCC cell line: role of AKT inhibition in bypassing this resistance. Int J Oncol. 38:189–200. 2011.PubMed/NCBI

22 

Dias JD, Guse K, Nokisalmi P, et al: Multimodal approach using oncolytic adenovirus, cetuximab, chemotherapy and radiotherapy in HNSCC low passage tumour cell cultures. Eur J Cancer. 46:625–635. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Wagenblast J, Baghi M, Arnoldner C, et al: Effect of bortezomib and cetuximab in EGF-stimulated HNSCC. Anticancer Res. 28:2239–2243. 2008.PubMed/NCBI

24 

Jouan-Hureaux V, Boura C, Merlin JL and Faivre B: Modulation of endothelial cell network formation in vitro by molecular signaling of head and neck squamous cell carcinoma (HNSCC) exposed to cetuximab. Microvasc Res. 83:131–137. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Lu Y, Liu P, Van den Bergh F, et al: Modulation of gene expression and cell-cycle signaling pathways by the EGFR inhibitor gefitinib (Iressa) in rat urinary bladder cancer. Cancer Prev Res. 5:248–259. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Normanno N, De Luca A, Maiello MR, et al: The MEK/MAPK pathway is involved in the resistance of breast cancer cells to the EGFR tyrosine kinase inhibitor gefitinib. J Cell Physiol. 207:420–427. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Milano G, Spano JP and Leyland-Jones B: EGFR-targeting drugs in combination with cytotoxic agents: from bench to bedside, a contrasted reality. Br J Cancer. 99:1–5. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Peng G, Wargovich MJ and Dixon DA: Anti-proliferative effects of green tea polyphenol EGCG on Ha-Ras-induced transformation of intestinal epithelial cells. Cancer Lett. 238:260–270. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Collins QF, Liu HY, Pi J, Liu Z, Quon MJ and Cao W: Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, suppresses hepatic gluconeogenesis through 5′-AMP-activated protein kinase. J Biol Chem. 282:30143–30149. 2007.PubMed/NCBI

30 

Guo S, Yang S, Taylor C and Sonenshein GE: Green tea polyphenol epigallocatechin-3 gallate (EGCG) affects gene expression of breast cancer cells transformed by the carcinogen 7,12-dimethylbenz[a]anthracene. J Nutr. 135:S2978–S2986. 2005.PubMed/NCBI

31 

Ahmed S, Wang N, Lalonde M, Goldberg VM and Haqqi TM: Green tea polyphenol epigallocatechin-3-gallate (EGCG) differentially inhibits interleukin-1 beta-induced expression of matrix metalloproteinase-1 and -13 in human chondrocytes. J Pharmacol Exp Ther. 308:767–773. 2004. View Article : Google Scholar

32 

Annabi B, Currie JC, Moghrabi A and Beliveau R: Inhibition of HuR and MMP-9 expression in macrophage-differentiated HL-60 myeloid leukemia cells by green tea polyphenol EGCg. Leuk Res. 31:1277–1284. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Liu L, Lai CQ, Nie L, et al: The modulation of endothelial cell gene expression by green tea polyphenol-EGCG. Mol Nutr Food Res. 52:1182–1192. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Siddiqui IA, Malik A, Adhami VM, et al: Green tea polyphenol EGCG sensitizes human prostate carcinoma LNCaP cells to TRAIL-mediated apoptosis and synergistically inhibits biomarkers associated with angiogenesis and metastasis. Oncogene. 27:2055–2063. 2008. View Article : Google Scholar

35 

Masuda M, Wakasaki T, Toh S, Shimizu M and Adachi S: Chemoprevention of head and neck cancer by green tea extract: EGCG-the role of EGFR signaling and ‘Lipid Raft’. J Oncol. 2011:5401482011.PubMed/NCBI

36 

Khan N and Mukhtar H: Multitargeted therapy of cancer by green tea polyphenols. Cancer Lett. 269:269–280. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Chen PN, Chu SC, Kuo WH, Chou MY, Lin JK and Hsieh YS: Epigallocatechin-3 gallate inhibits invasion, epithelial-mesenchymal transition, and tumor growth in oral cancer cells. J Agric Food Chem. 59:3836–3844. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Jiang L, Ji N, Zhou Y, et al: CAL 27 is an oral adenosquamous carcinoma cell line. Oral Oncol. 45:e204–e207. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Fan MJ, Lin YC, Shih HD, et al: Crude extracts of Agaricus brasiliensis induce apoptosis in human oral cancer CAL 27 cells through a mitochondria-dependent pathway. In Vivo. 25:355–366. 2011.PubMed/NCBI

40 

Chien MH, Ying TH, Hsieh YS, et al: Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity. Food Chem Toxicol. 50:558–566. 2012. View Article : Google Scholar

41 

Lai KC, Huang AC, Hsu SC, et al: Benzyl isothiocyanate (BITC) inhibits migration and invasion of human colon cancer HT29 cells by inhibiting matrix metalloproteinase-2/-9 and urokinase plasminogen (uPA) through PKC and MAPK signaling pathway. J Agric Food Chem. 58:2935–2942. 2010. View Article : Google Scholar

42 

Yu FS, Huang AC, Yang JS, et al: Safrole induces cell death in human tongue squamous cancer SCC-4 cells through mitochondria-dependent caspase activation cascade apoptotic signaling pathways. Environ Toxicol. 27:433–444. 2011.

43 

Troeberg L and Nagase H: Zymography of metalloproteinases. Curr Protoc Protein Sci. Chapter 21(Unit 21): 152004. View Article : Google Scholar

44 

Lin JJ, Hsu HY, Yang JS, et al: Molecular evidence of anti-leukemia activity of gypenosides on human myeloid leukemia HL-60 cells in vitro and in vivo using a HL-60 cells murine xenograft model. Phytomedicine. 18:1075–1085. 2011. View Article : Google Scholar : PubMed/NCBI

45 

Chang YC, Lai TY, Yu CS, et al: Emodin induces apoptotic death in murine myelomonocytic leukemia WEHI-3 cells in vitro and enhances phagocytosis in leukemia mice in vivo. Evid Based Complement Alternat Med. 2011:5235962011.PubMed/NCBI

46 

Chou ST, Peng HY, Chang CT, et al: Zanthoxylum ailanthoides Sieb and Zucc. extract inhibits growth and induces cell death through G2/M-phase arrest and activation of apoptotic signals in colo 205 human colon adenocarcinoma cells. Anticancer Res. 31:1667–1676. 2011.PubMed/NCBI

47 

Lu CC, Yang JS, Huang AC, et al: Chrysophanol induces necrosis through the production of ROS and alteration of ATP levels in J5 human liver cancer cells. Mol Nutr Food Res. 54:967–976. 2010. View Article : Google Scholar : PubMed/NCBI

48 

Chiang JH, Yang JS, Ma CY, et al: Danthron, an anthraquinone derivative, induces DNA damage and caspase cascades-mediated apoptosis in SNU-1 human gastric cancer cells through mitochondrial permeability transition pores and Bax-triggered pathways. Chem Res Toxicol. 24:20–29. 2011. View Article : Google Scholar

49 

Chung JG, Chang HL, Lin WC, Yeh FT and Hung CF: Effects of ibuprofen on arylamine N-acetyltransferase activity in human colon tumor cells. J Appl Toxicol. 19:1–6. 1999. View Article : Google Scholar : PubMed/NCBI

50 

Gardina PJ, Clark TA, Shimada B, et al: Alternative splicing and differential gene expression in colon cancer detected by a whole genome exon array. BMC Genomics. 7:3252006. View Article : Google Scholar : PubMed/NCBI

51 

Yeh MH, Tsai TC, Kuo HP, et al: Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells. Int J Oncol. 39:1221–1231. 2011.PubMed/NCBI

52 

Saeed AI, Sharov V, White J, et al: TM4: a free, open-source system for microarray data management and analysis. Biotechniques. 34:374–378. 2003.PubMed/NCBI

53 

Yang HL, Kuo YH, Tsai CT, et al: Anti-metastatic activities of Antrodia camphorata against human breast cancer cells mediated through suppression of the MAPK signaling pathway. Food Chem Toxicol. 49:290–298. 2011. View Article : Google Scholar

54 

Deng YT and Lin JK: EGCG inhibits the invasion of highly invasive CL1–5 lung cancer cells through suppressing MMP-2 expression via JNK signaling and induces G2/M arrest. J Agric Food Chem. 59:13318–13327. 2011.PubMed/NCBI

55 

Sok JC, Coppelli FM, Thomas SM, et al: Mutant epidermal growth factor receptor (EGFRvIII) contributes to head and neck cancer growth and resistance to EGFR targeting. Clin Cancer Res. 12:5064–5073. 2006. View Article : Google Scholar : PubMed/NCBI

56 

Rubin Grandis J, Melhem MF, Barnes EL and Tweardy DJ: Quantitative immunohistochemical analysis of transforming growth factor-alpha and epidermal growth factor receptor in patients with squamous cell carcinoma of the head and neck. Cancer. 78:1284–1292. 1996.

57 

Rubin Grandis J, Melhem MF, Gooding WE, et al: Levels of TGF-alpha and EGFR protein in head and neck squamous cell carcinoma and patient survival. J Natl Cancer Inst. 90:824–832. 1998.

58 

Zhang X, Zhang H, Tighiouart M, et al: Synergistic inhibition of head and neck tumor growth by green tea (−)-epigallocatechin-3-gallate and EGFR tyrosine kinase inhibitor. Int J Cancer. 123:1005–1014. 2008.

59 

Wu PP, Kuo SC, Huang WW, et al: (−)-Epigallocatechin gallate induced apoptosis in human adrenal cancer NCI-H295 cells through caspase-dependent and caspase-independent pathway. Anticancer Res. 29:1435–1442. 2009.

60 

Chen NG, Lu CC, Lin YH, et al: Proteomic approaches to study epigallocatechin gallate-provoked apoptosis of TSGH-8301 human urinary bladder carcinoma cells: roles of AKT and heat shock protein 27-modulated intrinsic apoptotic pathways. Oncol Rep. 26:939–947. 2011.

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Copy and paste a formatted citation
Spandidos Publications style
Chang C, Chang P, Tu M, Lu C, Kuo S, Amagaya S, Lee C, Jao H, Chen MY, Yang J, Yang J, et al: Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2. Oncol Rep 28: 1799-1807, 2012.
APA
Chang, C., Chang, P., Tu, M., Lu, C., Kuo, S., Amagaya, S. ... Yang, J. (2012). Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2. Oncology Reports, 28, 1799-1807. https://doi.org/10.3892/or.2012.1991
MLA
Chang, C., Chang, P., Tu, M., Lu, C., Kuo, S., Amagaya, S., Lee, C., Jao, H., Chen, M. Y., Yang, J."Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2". Oncology Reports 28.5 (2012): 1799-1807.
Chicago
Chang, C., Chang, P., Tu, M., Lu, C., Kuo, S., Amagaya, S., Lee, C., Jao, H., Chen, M. Y., Yang, J."Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2". Oncology Reports 28, no. 5 (2012): 1799-1807. https://doi.org/10.3892/or.2012.1991
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x
Spandidos Publications style
Chang C, Chang P, Tu M, Lu C, Kuo S, Amagaya S, Lee C, Jao H, Chen MY, Yang J, Yang J, et al: Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2. Oncol Rep 28: 1799-1807, 2012.
APA
Chang, C., Chang, P., Tu, M., Lu, C., Kuo, S., Amagaya, S. ... Yang, J. (2012). Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2. Oncology Reports, 28, 1799-1807. https://doi.org/10.3892/or.2012.1991
MLA
Chang, C., Chang, P., Tu, M., Lu, C., Kuo, S., Amagaya, S., Lee, C., Jao, H., Chen, M. Y., Yang, J."Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2". Oncology Reports 28.5 (2012): 1799-1807.
Chicago
Chang, C., Chang, P., Tu, M., Lu, C., Kuo, S., Amagaya, S., Lee, C., Jao, H., Chen, M. Y., Yang, J."Epigallocatechin gallate sensitizes CAL-27 human oral squamous cell carcinoma cells to the anti-metastatic effects of gefitinib (Iressa) via synergistic suppression of epidermal growth factor receptor and matrix metalloproteinase-2". Oncology Reports 28, no. 5 (2012): 1799-1807. https://doi.org/10.3892/or.2012.1991
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