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Article

Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration

  • Authors:
    • Chang-I Kung
    • Cheng-Yi Chen
    • Chih-Chang Yang
    • Cheng-Yu Lin
    • Tien-Hua Chen
    • Hwai-Shi Wang
  • View Affiliations / Copyright

    Affiliations: Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, Taipei, Taiwan, R.O.C. , Department of Surgery, Veterans General Hospital, Taipei, Taiwan, R.O.C.
  • Pages: 1808-1814
    |
    Published online on: August 24, 2012
       https://doi.org/10.3892/or.2012.1993
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Abstract

Hyaluronan (HA), a component of the extracellular matrix, plays an important role in cell-cell adhesion and cell migration. Membrane type 1-matrix metalloproteinase (MT1‑MMP) is often expressed in invasive cancer cells. CD44, a transmembrane receptor for HA, is implicated in various adhesion-dependent cellular processes including cell migration, tumor cell metastasis and invasion. Previous studies have shown that CD44 is highly expressed in cancer cells and may be proteolytically cleaved at the ectodomain by MT1-MMP; this process of inducing CD44 cleavage plays a critical role in cancer cell migration. We hypothesized that HA modulates MT1-MMP expression to facilitate breast cancer cell migration. Flow cytometry, real-time PCR, western blotting and immunofluorescence staining were used to quantify HA-induced MT1-MMP expression in breast cancer cells. In order to validate the relevance of cell migration and HA-induced MT1-MMP, we analyzed the cell migration via matrigel-coated transwell. We found that after HA oligosaccharide (6.5 kDA) stimulation, MT1-MMP expression in the membrane of breast cancer cells was increased. In response to HA oligosaccharide stimulation, significant upregulation of MT1-MMP mRNA occurred. Our data also provide evidence that HA oligosaccharide enhances MT1-MMP; the elevated expression of MT1-MMP confers enhanced CD44 cleavage and cell migration. In conclusion, we have identified a new function of HA in the induction of MT1-MMP expression in breast cancer cell lines and CD44 cleavage to increase cell migration during the invasion process. The HA oligosaccharide-induced MT1-MMP expression in breast cancer cells may be a critical step in the formation of metastatic colonies.
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Copy and paste a formatted citation
Spandidos Publications style
Kung C, Chen C, Yang C, Lin C, Chen T and Wang H: Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration. Oncol Rep 28: 1808-1814, 2012.
APA
Kung, C., Chen, C., Yang, C., Lin, C., Chen, T., & Wang, H. (2012). Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration. Oncology Reports, 28, 1808-1814. https://doi.org/10.3892/or.2012.1993
MLA
Kung, C., Chen, C., Yang, C., Lin, C., Chen, T., Wang, H."Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration". Oncology Reports 28.5 (2012): 1808-1814.
Chicago
Kung, C., Chen, C., Yang, C., Lin, C., Chen, T., Wang, H."Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration". Oncology Reports 28, no. 5 (2012): 1808-1814. https://doi.org/10.3892/or.2012.1993
Copy and paste a formatted citation
x
Spandidos Publications style
Kung C, Chen C, Yang C, Lin C, Chen T and Wang H: Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration. Oncol Rep 28: 1808-1814, 2012.
APA
Kung, C., Chen, C., Yang, C., Lin, C., Chen, T., & Wang, H. (2012). Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration. Oncology Reports, 28, 1808-1814. https://doi.org/10.3892/or.2012.1993
MLA
Kung, C., Chen, C., Yang, C., Lin, C., Chen, T., Wang, H."Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration". Oncology Reports 28.5 (2012): 1808-1814.
Chicago
Kung, C., Chen, C., Yang, C., Lin, C., Chen, T., Wang, H."Enhanced membrane-type 1 matrix metalloproteinase expression by hyaluronan oligosaccharides in breast cancer cells facilitates CD44 cleavage and tumor cell migration". Oncology Reports 28, no. 5 (2012): 1808-1814. https://doi.org/10.3892/or.2012.1993
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