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November 2012 Volume 28 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer

  • Authors:
    • Yufeng Wang
    • Yasuhiro Kuramitsu
    • Tomio Ueno
    • Nobuaki Suzuki
    • Shigefumi Yoshino
    • Norio Iizuka
    • Xiulian Zhang
    • Junko Akada
    • Masaaki Oka
    • Kazuyuki Nakamura
  • View Affiliations

    Affiliations: Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Japan, Department of Digestive Surgery of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Ube, Japan, The Institute of Human Nutrition, Medical College of Qingdao University, Qingdao, P.R. China
  • Published online on: August 30, 2012     https://doi.org/10.3892/or.2012.2004
  • Pages: 1845-1850
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Abstract

Pancreatic cancer (PC) is characterized by rapid tumor spread, and very few patients with PC survive for more than 5 years. It is imperative to discover additional diagnostic biomarkers or specific therapeutic targets in order to improve the treatment of patients with PC. In search for useful biomarkers, we analyzed ten pairs of non-cancerous and cancer tissues from patients with PC by two-dimensional gel electrophoresis (2-DE). Nineteen protein spots showed differential expression on 2-DE gels between the cancer and non-cancerous tissues. Six upregulated protein spots were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) as calreticulin, glutathione synthetase, stathmin, vinculin, α-enolase and glyceraldehyde-3-phosphate dehydrogenase. Western blotting demonstrated that vinculin was predominantly expressed in the pancreatic cancer tissues compared with to non-cancerous tissues. Our findings indicate that vinculin may be a clinically useful biomarker of PC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Kuramitsu Y, Ueno T, Suzuki N, Yoshino S, Iizuka N, Zhang X, Akada J, Oka M, Nakamura K, Nakamura K, et al: Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer. Oncol Rep 28: 1845-1850, 2012.
APA
Wang, Y., Kuramitsu, Y., Ueno, T., Suzuki, N., Yoshino, S., Iizuka, N. ... Nakamura, K. (2012). Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer. Oncology Reports, 28, 1845-1850. https://doi.org/10.3892/or.2012.2004
MLA
Wang, Y., Kuramitsu, Y., Ueno, T., Suzuki, N., Yoshino, S., Iizuka, N., Zhang, X., Akada, J., Oka, M., Nakamura, K."Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer". Oncology Reports 28.5 (2012): 1845-1850.
Chicago
Wang, Y., Kuramitsu, Y., Ueno, T., Suzuki, N., Yoshino, S., Iizuka, N., Zhang, X., Akada, J., Oka, M., Nakamura, K."Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer". Oncology Reports 28, no. 5 (2012): 1845-1850. https://doi.org/10.3892/or.2012.2004
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Kuramitsu Y, Ueno T, Suzuki N, Yoshino S, Iizuka N, Zhang X, Akada J, Oka M, Nakamura K, Nakamura K, et al: Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer. Oncol Rep 28: 1845-1850, 2012.
APA
Wang, Y., Kuramitsu, Y., Ueno, T., Suzuki, N., Yoshino, S., Iizuka, N. ... Nakamura, K. (2012). Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer. Oncology Reports, 28, 1845-1850. https://doi.org/10.3892/or.2012.2004
MLA
Wang, Y., Kuramitsu, Y., Ueno, T., Suzuki, N., Yoshino, S., Iizuka, N., Zhang, X., Akada, J., Oka, M., Nakamura, K."Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer". Oncology Reports 28.5 (2012): 1845-1850.
Chicago
Wang, Y., Kuramitsu, Y., Ueno, T., Suzuki, N., Yoshino, S., Iizuka, N., Zhang, X., Akada, J., Oka, M., Nakamura, K."Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer". Oncology Reports 28, no. 5 (2012): 1845-1850. https://doi.org/10.3892/or.2012.2004
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