Enhanced proliferation of human hepatoma cells by PAR-2 agonists via the ERK/AP-1 pathway

Xie,Liqun; Zheng,Yanmin; Li,Xuan; Zhao,Junyan; Chen,Xiaoyi; Chen,Li; Zhou,Jing; Hai,Ou; Li,Fei

doi:10.3892/or.2012.2007

Enhanced proliferation of human hepatoma cells by PAR-2 agonists via the ERK/AP-1 pathway

Authors:
- Liqun Xie
- Yanmin Zheng
- Xuan Li
- Junyan Zhao
- Xiaoyi Chen
- Li Chen
- Jing Zhou
- Ou Hai
- Fei Li
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Affiliations: Department of Gastroenterology, Affiliated Hospital, Logistics College of the Chinese People's Armed Police Forces, Tianjin 300162, P.R. China, Department of Cell Biology, Logistics College of the Chinese People's Armed Police Forces, Tianjin 300162, P.R. China, Department of Inner Mongolia Corps Clinic, Chinese People's Armed Police Forces, Inner Mongolia 010050, P.R. China
Published online on: August 31, 2012 https://doi.org/10.3892/or.2012.2007
Pages: 1665-1672

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Abstract

To investigate the expression and role of PAR-2 in the proliferation of the human hepatoma cell line HepG2, PAR-2 protein and mRNA expression were evaluated by immuno-histochemistry, immunofluorescence and RT-PCR analysis. The signaling pathways downstream of PAR-2 activation that lead to hepatoma cell proliferation were analyzed. The results showed that PAR-2 is expressed in human hepatoma cells and PAR-2 mRNA expression was found to be upregulated in cells treated with trypsin or SLIGKV-NH2 (P<0.001). The proliferation rate of HepG2 cells treated with trypsin or SLIGKV-NH2 was significantly increased (P<0.001). The percentage of S phase, G2/M phase and the proliferation index (PI) of HepG2 cells treated with trypsin or SLIGKV-NH2 were significantly elevated (P<0.001). The proliferative responses of HepG2 to trypsin and SLIGKV-NH2 were associated with the upregulation of c-fos and PCNA, which were significantly blocked by PD98059 pretreatment. In conclusion, our results indicate that PAR-2 enhances proliferation of human hepatoma cells possibly via the ERK/AP-1 pathway.

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