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Article

Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features

  • Authors:
    • Joo Heon Kim
    • Mee Young Park
    • Chang Nam Kim
    • Kyo Hyun Kim
    • Ho Bum Kang
    • Kwang Dong Kim
    • Jae Wha Kim
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Eulji University School of Medicine, Daejeon 301‑070, Republic of Korea, Department of Surgery a, Eulji University School of Medicine, Daejeon 301‑070, Republic of Korea, Department of Preventive Medicine, Eulji University School of Medicine, Daejeon 301‑070, Republic of Korea, Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea, Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • Pages: 1701-1708
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    Published online on: September 3, 2012
       https://doi.org/10.3892/or.2012.2012
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Abstract

Based on a previous finding that endothelial cell-specific molecule-1 (ESM-1) is a potential serum marker for colorectal cancer (CRC), the aim of this study was to clarify the clinicopathological significance of ESM-1 expression in CRC, and to explore the correlation between ESM-1 and HIF-1α in the tumorigenesis of CRC related to hypoxic conditions. ESM-1 mRNA expression was examined in CRC and corresponding normal mucosal tissues by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR. This experiment confirmed that ESM-1 levels were high in CRC. We screened the tissue samples of 143 CRC patients. By immunohistochemistry, we determined that the ESM-1 immunoreactivity was significantly correlated with the tumor size, depth of invasion, nodal status, distant metastasis and Dukes' stage, and was an independent prognostic factor for disease recurrence and worse survival outcome (P=0.001). The modulation of ESM-1 under hypoxia was investigated, and it was confirmed that ESM-1 expression was induced by HIF1-α and significantly attenuated by small interfering RNA (siRNA) targeting HIF-1α in CRC cells. These results showed that ESM-1 is significantly overexpressed, which is regulated by HIF-1α in CRC patients, and can be used as a potential biomarker and a therapeutic target for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Kim JH, Park MY, Kim CN, Kim KH, Kang HB, Kim KD and Kim JW: Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features. Oncol Rep 28: 1701-1708, 2012.
APA
Kim, J.H., Park, M.Y., Kim, C.N., Kim, K.H., Kang, H.B., Kim, K.D., & Kim, J.W. (2012). Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features. Oncology Reports, 28, 1701-1708. https://doi.org/10.3892/or.2012.2012
MLA
Kim, J. H., Park, M. Y., Kim, C. N., Kim, K. H., Kang, H. B., Kim, K. D., Kim, J. W."Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features". Oncology Reports 28.5 (2012): 1701-1708.
Chicago
Kim, J. H., Park, M. Y., Kim, C. N., Kim, K. H., Kang, H. B., Kim, K. D., Kim, J. W."Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features". Oncology Reports 28, no. 5 (2012): 1701-1708. https://doi.org/10.3892/or.2012.2012
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JH, Park MY, Kim CN, Kim KH, Kang HB, Kim KD and Kim JW: Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features. Oncol Rep 28: 1701-1708, 2012.
APA
Kim, J.H., Park, M.Y., Kim, C.N., Kim, K.H., Kang, H.B., Kim, K.D., & Kim, J.W. (2012). Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features. Oncology Reports, 28, 1701-1708. https://doi.org/10.3892/or.2012.2012
MLA
Kim, J. H., Park, M. Y., Kim, C. N., Kim, K. H., Kang, H. B., Kim, K. D., Kim, J. W."Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features". Oncology Reports 28.5 (2012): 1701-1708.
Chicago
Kim, J. H., Park, M. Y., Kim, C. N., Kim, K. H., Kang, H. B., Kim, K. D., Kim, J. W."Expression of endothelial cell-specific molecule-1 regulated by hypoxia inducible factor-1α in human colon carcinoma: Impact of ESM-1 on prognosis and its correlation with clinicopathological features". Oncology Reports 28, no. 5 (2012): 1701-1708. https://doi.org/10.3892/or.2012.2012
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