Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10

  • Authors:
    • Kazuhiro Iguchi
    • Yuri Hayakawa
    • Kenichiro Ishii
    • Kaoru Matsumoto
    • Shigeyuki Usui
    • Yoshiki Sugimura
    • Kazuyuki Hirano
  • View Affiliations

  • Published online on: September 17, 2012     https://doi.org/10.3892/or.2012.2039
  • Pages: 2009-2015
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Abstract

Intratumoral regions of low extracellular pH and low nutrition are common features of solid tumors. Although cancer cells normally die when they are removed from their environment, a small population of cells survive. In the present study, the subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH/low nutrient-resistant properties were examined. LNCaP-F10 cells were grown under low-pH/low-nutrient conditions, which caused cell death of the LNCaP cells. The cell death was associated with oligonucleosomal DNA fragmentation and poly (ADP-ribose) polymerase (PARP) cleavage, indicating that low-pH/low-nutrient induced apoptosis in these cells. Significant differences in the expression of BCL2, BIRC5 and DAPK1 were detected between LNCaP-F10 and LNCaP cells. Tumor growth caused by implantation of LNCaP-F10 cells into the renal subcapsular space of nude mice in the absence or presence of prostate stromal cell stimulation was greater than that caused by implantation of LNCaP cells. LNCaP-F10 cells were resistant to apoptosis induced by an environment of low-pH/low-nutrient in vitro, and displayed malignant potential in vivo.
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December 2012
Volume 28 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Iguchi K, Hayakawa Y, Ishii K, Matsumoto K, Usui S, Sugimura Y and Hirano K: Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10. Oncol Rep 28: 2009-2015, 2012
APA
Iguchi, K., Hayakawa, Y., Ishii, K., Matsumoto, K., Usui, S., Sugimura, Y., & Hirano, K. (2012). Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10. Oncology Reports, 28, 2009-2015. https://doi.org/10.3892/or.2012.2039
MLA
Iguchi, K., Hayakawa, Y., Ishii, K., Matsumoto, K., Usui, S., Sugimura, Y., Hirano, K."Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10". Oncology Reports 28.6 (2012): 2009-2015.
Chicago
Iguchi, K., Hayakawa, Y., Ishii, K., Matsumoto, K., Usui, S., Sugimura, Y., Hirano, K."Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10". Oncology Reports 28, no. 6 (2012): 2009-2015. https://doi.org/10.3892/or.2012.2039