UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer

  • Authors:
    • Yasuhide Kofunato
    • Kensuke Kumamoto
    • Katsuharu Saitou
    • Suguru Hayase
    • Hirokazu Okayama
    • Kotaro Miyamoto
    • Yu Sato
    • Kyoko Katakura
    • Izumi Nakamura
    • Shinji Ohki
    • Yoshihisa Koyama
    • Motoko Unoki
    • Seiichi Takenoshita
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  • Published online on: September 27, 2012     https://doi.org/10.3892/or.2012.2064
  • Pages: 1997-2002
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Abstract

Ubiquitin-like with PHD and ring-finger domain 1 (UHRF1) binds to methylated promoters of a number of tumor-suppressor genes, including p16INK4A and p14ARF, by forming complexes with DNA methyltransferases and HDAC1, resulting in the induction of carcinogenesis. Altered UHRF1 expression has been demonstrated in various types of cancers. Previous reports indicate that UHRF1 expression is regulated by E2F-1 expression. We investigated UHRF1 expression using immunohistochemical staining in 231 colorectal cancer and 40 adenoma specimens, analyzed the relationship between UHRF1 expression and clinicopathological findings and the association between UHRF1 and E2F-1 expression. To better understand the biological function of UHRF1 in colorectal cancer, knockdown of UHRF1 expression was performed using siRNA methods. High UHRF1 expression was observed in 152 of 231 (65.8%) colorectal cancer patients, and was detected in 35 of 40 adenoma specimens samples (87.5%). UHRF1 staining was detected in the nucleus of cancer cells, while it was not detected in colonic normal mucosa. High UHRF1 expression was significantly observed in right compared with left hemicolon cancer (p=0.008). Moreover, high UHRF1 expression tended to be associated with depth of invasion (p=0.051). UHRF1 expression was significantly associated with E2F-1 expression (p<0.0001). Knockdown of UHRF1 expression suppressed cellular growth in colon cancer cell lines, HCT116 and SW620. In conclusion, we demonstrated that UHRF1 expression was upregulated in approximately two-thirds of colorectal cancer specimens and was particularly expressed in right compared with left hemicolon cancer. Moreover, knockdown of UHRF1 expression induced growth inhibition in colon cancer cell lines. UHRF1 may be involved in cellular proliferation and molecular pathogenesis of colorectal cancer in the right hemicolon.
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December 2012
Volume 28 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kofunato Y, Kumamoto K, Saitou K, Hayase S, Okayama H, Miyamoto K, Sato Y, Katakura K, Nakamura I, Ohki S, Ohki S, et al: UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer. Oncol Rep 28: 1997-2002, 2012.
APA
Kofunato, Y., Kumamoto, K., Saitou, K., Hayase, S., Okayama, H., Miyamoto, K. ... Takenoshita, S. (2012). UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer. Oncology Reports, 28, 1997-2002. https://doi.org/10.3892/or.2012.2064
MLA
Kofunato, Y., Kumamoto, K., Saitou, K., Hayase, S., Okayama, H., Miyamoto, K., Sato, Y., Katakura, K., Nakamura, I., Ohki, S., Koyama, Y., Unoki, M., Takenoshita, S."UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer". Oncology Reports 28.6 (2012): 1997-2002.
Chicago
Kofunato, Y., Kumamoto, K., Saitou, K., Hayase, S., Okayama, H., Miyamoto, K., Sato, Y., Katakura, K., Nakamura, I., Ohki, S., Koyama, Y., Unoki, M., Takenoshita, S."UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer". Oncology Reports 28, no. 6 (2012): 1997-2002. https://doi.org/10.3892/or.2012.2064