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Article

SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells

  • Authors:
    • Peng Zhang
    • Guanmin Jiang
    • Jiao Gao
    • Lingling Li
    • Jun Du
    • Xingyuan Jiao
  • View Affiliations / Copyright

    Affiliations: Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, P.R. China, Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Science, Sun Yat-sen University, University Town, Guangzhou 510006, P.R. China, Department of Hepatobiliary Surgery, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, P.R. China
  • Pages: 269-275
    |
    Published online on: October 8, 2012
       https://doi.org/10.3892/or.2012.2073
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Abstract

The aim of the present study was to investigate the role of the JAK/STAT1 signaling pathway in suberoylanilide hydroxamic acid (SAHA)-mediated down-regulation of indoleamine 2,3-dioxygenase (IDO) in gallbladder carcinoma cells. We treated SGC-996 gallbladder carcinoma cells with IFN-γ and SAHA. Western blotting was used to detect the expression of IDO, signal transducer and activator of transcription 1 (STAT1) phosphorylation and interferon regulatory factor genes-1 (IRF-1). Confocal microscopy analysis was used to detect STAT1 translocation. Transient transfection and reporter gene assay was used for detecting the activation of γ-activated sites (GAS) and interferon-stimulated response elements (ISRE). The results revealed that IDO was expressed in SGC-996 cells in a dose- and time-dependent manner when stimulated with IFN-γ and SAHA down-regulated the expression of IDO induced by IFN-γ in a dose-dependent manner. SAHA blocked the expression of IRF-1 induced by IFN-γ and SAHA inhibited IFN-γ-induced STAT1 phosphorylation and nuclear translocation. In addition, SAHA down-regulated IFN-γ-induced activation of GAS and ISRE. In conclusion, SAHA down-regulated IDO expression via inhibition of the activation of members of the JAK/STAT1 signaling pathway. Therefore, regulation of the JAK/STAT1 signaling pathway may provide a new gallbladder carcinoma immunotherapeutic strategy to break tumor immune tolerance.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang P, Jiang G, Gao J, Li L, Du J and Jiao X: SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells. Oncol Rep 29: 269-275, 2013.
APA
Zhang, P., Jiang, G., Gao, J., Li, L., Du, J., & Jiao, X. (2013). SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells. Oncology Reports, 29, 269-275. https://doi.org/10.3892/or.2012.2073
MLA
Zhang, P., Jiang, G., Gao, J., Li, L., Du, J., Jiao, X."SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells". Oncology Reports 29.1 (2013): 269-275.
Chicago
Zhang, P., Jiang, G., Gao, J., Li, L., Du, J., Jiao, X."SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells". Oncology Reports 29, no. 1 (2013): 269-275. https://doi.org/10.3892/or.2012.2073
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang P, Jiang G, Gao J, Li L, Du J and Jiao X: SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells. Oncol Rep 29: 269-275, 2013.
APA
Zhang, P., Jiang, G., Gao, J., Li, L., Du, J., & Jiao, X. (2013). SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells. Oncology Reports, 29, 269-275. https://doi.org/10.3892/or.2012.2073
MLA
Zhang, P., Jiang, G., Gao, J., Li, L., Du, J., Jiao, X."SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells". Oncology Reports 29.1 (2013): 269-275.
Chicago
Zhang, P., Jiang, G., Gao, J., Li, L., Du, J., Jiao, X."SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells". Oncology Reports 29, no. 1 (2013): 269-275. https://doi.org/10.3892/or.2012.2073
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