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Article

Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo

  • Authors:
    • Min Li
    • Jie Yin
    • Ning Mao
    • Lingya Pan
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Beijing Hospital, Dongdan, Beijing 100730, P.R. China, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, P.R. China, Department of Cell Biology, Institute of Basic Medical Science, Beijing 100850, P.R. China, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730
  • Pages: 58-66
    |
    Published online on: October 12, 2012
       https://doi.org/10.3892/or.2012.2078
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Abstract

The acquisition of chemoresistance is a major therapeutic obstacle in the clinical treatment of ovarian cancer. Diagnosing chemoresistance in ovarian cancer patients at an early stage is necessary for prognosis, but at present significant proteins related to chemoresistance that may indicate and reverse chemoresistance in human ovarian cancer have not been discovered. In this study, we demonstrated that the protein, phosphorylated cofilin 1 (p-CFL1) correlates with taxol resistance in human ovarian cancer cells. The total proteins of two sensitive (SKOV3 and A2780) and three taxol-resistant (SKOV3/TR2500, SKOV3/TR30 and A2780/TR) human ovarian cancer cell lines were isolated by 2-dimensional gel electrophoresis (2-DE). Twenty-two protein spots in all samples were revealed to be significantly different in spot intensity by statistical analysis, 16 of which were identified using matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS). Cofilin 1 (CFL1) was selected as a candidate which may play an important role in taxol resistance. Higher expression levels of p-CFL1 in taxol-resistant cells were demonstrated. Furthermore, the levels of p-CFL1 in primary human ovarian cancer tissues were shown to be significantly higher in chemoresistant cases compared to those in chemosensitive ones. These findings suggest that p-CFL1 is upregulated in taxol-resistant ovarian cancer and this upregulation is a chara­cteristic of taxol resistance both in vitro and in vivo. However, the mechanisms need to be further elucidated.
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Copy and paste a formatted citation
Spandidos Publications style
Li M, Yin J, Mao N and Pan L: Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo. Oncol Rep 29: 58-66, 2013.
APA
Li, M., Yin, J., Mao, N., & Pan, L. (2013). Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo. Oncology Reports, 29, 58-66. https://doi.org/10.3892/or.2012.2078
MLA
Li, M., Yin, J., Mao, N., Pan, L."Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo". Oncology Reports 29.1 (2013): 58-66.
Chicago
Li, M., Yin, J., Mao, N., Pan, L."Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo". Oncology Reports 29, no. 1 (2013): 58-66. https://doi.org/10.3892/or.2012.2078
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Yin J, Mao N and Pan L: Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo. Oncol Rep 29: 58-66, 2013.
APA
Li, M., Yin, J., Mao, N., & Pan, L. (2013). Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo. Oncology Reports, 29, 58-66. https://doi.org/10.3892/or.2012.2078
MLA
Li, M., Yin, J., Mao, N., Pan, L."Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo". Oncology Reports 29.1 (2013): 58-66.
Chicago
Li, M., Yin, J., Mao, N., Pan, L."Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo". Oncology Reports 29, no. 1 (2013): 58-66. https://doi.org/10.3892/or.2012.2078
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