Effect of tumor gangliosides on tyrosine phosphorylation of p125FAK in platelet adhesion to collagen

  • Authors:
    • Yi-Xin Chen
    • Xiao-Wen Chen
    • Chang-Gang Li
    • Li-Jie Yue
    • Hui-Rong Mai
    • Fei-Qiu Wen
  • View Affiliations

  • Published online on: October 19, 2012     https://doi.org/10.3892/or.2012.2092
  • Pages: 343-348
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Abstract

The exact mechanisms as to how platelets influence blood-borne metastasis remain poorly understood. Gangliosides, sialic acid-containing glycosphingolipids, are associated with tumor progression and metastasis in humans. Gangliosides isolated from tumor cells promote collagen-stimulated platelet aggregation and ATP secretion and enhance platelet adhesion to immobilized collagen. Gangliosides interact with a number of cell surface receptors including integrin receptors. In this study, we examined the effects of α2β1 integrin-mediated platelet adhesion to collagen and phosphotyrosine signaling of focal adhesion kinase, p125FAK (FAK). platelets pre-incubated with neuroblastoma tumor gangliosides (NBTGs) or their major component GD2 (disialoganglioside) were more adherent to immobilized collagen (OD570 0.43±0.12, 0.39±0.13) compared to platelets pre-incubated with MTB (0.14±0.06, p<0.001); the effect of NBTGs was blocked by F-17 anti-α2 antibody. Pre-incubation of platelets with NBTGs resulted in a marked increase in the phosphotyrosine content of p125FAK in the adherent platelets compared to the MTB-pre-incubated adherent platelets. F-17 anti-α2 antibody decreased protein tyrosine phosphorylation of NBTG-incubated platelets adherent to collagen. These results indicate that the tumor gangliosides enhance platelet adhesion to extracellular matrix collagen by upregulating integrin α2β1-mediated tyrosine phosphorylation of p125FAK, thereby providing insight into how this interaction may be involved in neuroblastoma metastasis.
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January 2013
Volume 29 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen Y, Chen X, Li C, Yue L, Mai H and Wen F: Effect of tumor gangliosides on tyrosine phosphorylation of p125FAK in platelet adhesion to collagen. Oncol Rep 29: 343-348, 2013
APA
Chen, Y., Chen, X., Li, C., Yue, L., Mai, H., & Wen, F. (2013). Effect of tumor gangliosides on tyrosine phosphorylation of p125FAK in platelet adhesion to collagen. Oncology Reports, 29, 343-348. https://doi.org/10.3892/or.2012.2092
MLA
Chen, Y., Chen, X., Li, C., Yue, L., Mai, H., Wen, F."Effect of tumor gangliosides on tyrosine phosphorylation of p125FAK in platelet adhesion to collagen". Oncology Reports 29.1 (2013): 343-348.
Chicago
Chen, Y., Chen, X., Li, C., Yue, L., Mai, H., Wen, F."Effect of tumor gangliosides on tyrosine phosphorylation of p125FAK in platelet adhesion to collagen". Oncology Reports 29, no. 1 (2013): 343-348. https://doi.org/10.3892/or.2012.2092