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Article

4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells

  • Authors:
    • Teruyoshi Saito
    • Dai Tamura
    • Tatsuro Nakamura
    • Yukihisa Makita
    • Hitomi Ariyama
    • Keiji Komiyama
    • Tomoko Yoshihara
    • Ryuji Asano
  • View Affiliations / Copyright

    Affiliations: Laboratory of Veterinary Pharmacology, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan, Laboratory of Veterinary Pharmacology, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan
  • Pages: 335-342
    |
    Published online on: October 23, 2012
       https://doi.org/10.3892/or.2012.2100
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Abstract

Hyaluronan (HA), a major component of the extracellular matrix (ECM), is synthesized by HA synthase (HAS) 1, HAS2 and HAS3 and is intricately involved in cell growth and metastasis. The HA synthesis inhibitor 4-methylumbelliferone (4-MU) has been reported to exhibit anticancer properties in various types of malignant tumors. However, the underlying mechanisms at the molecular and cellular levels remain unclear. In this study, to establish an animal model for studying the function of HA in human breast cancer, we investigated the antitumor effects of 4-MU using canine mammary tumor (CF33) cells. First, we investigated the effects of 4-MU on HA production in CF33 cells. Quantitative analysis of HA in culture media showed that 4-MU inhibited HA synthesis, accompanied by downregulation of HAS2 mRNA levels, in a dose-dependent manner at 24-72 h. Additionally, we observed a 4-MU-mediated decrease in the extent of the cell-associated HA matrix. We examined the effect of 4-MU on cell growth and apoptosis in CF33 cells. 4-MU markedly inhibited cell proliferation and induced apoptosis in CF33 cells. In particular, our experiments showed that the mechanism of 4-MU-induced apoptosis in CF33 cells involved increased levels of expression of pro-apoptotic BAX mRNA and protein molecules. These data suggest that 4-MU may be a candidate therapeutic agent for the treatment of canine mammary tumors. Furthermore, this study provides the first indication that the canine mammary tumor may be a suitable model for comparative study of the function of HA in human breast cancer.
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View References

1 

Priester WA and Mantel N: Occurrence of tumors in domestic animals. Data from 12 United States and Canadian colleges of veterinary medicine. J Natl Cancer Inst. 47:1333–1344. 1971.

2 

Sorenmo K: Canine mammary gland tumors. Vet Clin North Am Small Anim Pract. 33:573–596. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Graham JC and Myers RK: The prognostic significance of angiogenesis in canine mammary tumors. J Vet Intern Med. 13:416–418. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Nieto A, Pena L, Perez-Alenza MD, Sanchez MA, Flores JM and Castano M: Immunohistologic detection of estrogen receptor alpha in canine mammary tumors: clinical and pathologic associations and prognostic significance. Vet Pathol. 37:239–247. 2000. View Article : Google Scholar

5 

Uva P, Aurisicchio L, Watters J, Loboda A, Kulkarni A, Castle J, Palombo F, Viti V, Mesiti G, Zappulli V, Marconato L, Abramo F, Ciliberto G, Lahm A, La Monica N and de Rinaldis E: Comparative expression pathways analysis of human and canine mammary tumors. BMC Genomics. 10:1352009. View Article : Google Scholar : PubMed/NCBI

6 

Standberg JD and Goodman DG: Animal model of human disease: canine mammary neoplasia. Am J Pathol. 75:225–228. 1974.PubMed/NCBI

7 

Pinho SS, Carvalho S, Cabral J, Reis CA and Gartner F: Canine tumors: a spontaneous animal model of human carcinogenesis. Transl Res. 159:165–172. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Weismann B, Rapport MM, Linker A and Meyer K: Isolation of the aldobionic acid of umbilical cord hyaluronic acid. J Biol Chem. 205:205–211. 1953.PubMed/NCBI

9 

Toole BP: Hyaluronan: from extracellular glue to pericellular cue. Nat Rev Cancer. 4:528–539. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Turley EA, Noble PW and Bourguignon LY: Signaling properties of hyaluronan receptors. J Biol Chem. 277:4589–4592. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Fraser JR, Laurent TC and Laurent UB: Hyaluronan: its nature, distribution, functions and turnover. J Intern Med. 242:27–33. 1997. View Article : Google Scholar : PubMed/NCBI

12 

Menzel E and Farr C: Hyaluronidase and its substrate hyaluronan: biochemistry, biological activities and therapeutic uses. Cancer Lett. 131:3–11. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Auvinen P, Tammi RH, Parkinen J, Tammi M, Agren U, Johansson R, Hirvikoski P, Eskelinen M and Kosma VM: Hyaluronan in peritumoral stroma and malignant cells associates with breast cancer spreading and predicts survival. Am J Pathol. 156:529–536. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Anttila MA, Tammi RH, Tammi MI, Syrjanen KJ, Saarikoski SV and Kosma VM: High levels of stromal hyaluronan predict poor disease outcome in epithelial ovarian cancer. Cancer Res. 60:150–155. 2000.PubMed/NCBI

15 

Lipponen P, Aaltomaa S, Tammi R, Tammi M, Agren U and Kosma VM: High stromal hyaluronan level is associated with poor differentiation and metastasis in prostate cancer. Eur J Cancer. 37:849–856. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Golshani R, Lopez L, Estrella V, Kramer M, Iida N and Lokeshwar VB: Hyaluronic acid synthase-1 expression regulates bladder cancer growth, invasion, and angiogenesis through CD44. Cancer Res. 68:483–491. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Udabage L, Brownlee GR, Waltham M, Blick T, Walker EC, Heldin P, Nilsson SK, Thompson EW and Brown TJ: Antisense-mediated suppression of hyaluronan synthase-2 inhibits the tumorigenesis and progression of breast cancer. Cancer Res. 65:6139–6150. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Liu N, Gao F, Han Z, Xu X, Underhill CB and Zhang L: Hyaluronan synthase 3 overexpression promotes the growth of TSU prostate cancer cells. Cancer Res. 61:5207–5214. 2001.PubMed/NCBI

19 

Cichy J and Pure E: The liberation of CD44. J Cell Biol. 161:839–843. 2003. View Article : Google Scholar

20 

Cheung WF, Cruz TF and Turley EA: Receptor for hyaluronan-mediated motility (RHAMM), a hyaladherin that regulates cell responses to growth factors. Biochem Soc Trans. 27:135–142. 1999.PubMed/NCBI

21 

Itano N, Zhuo L and Kimata K: Impact of the hyaluronan-rich tumor microenvironment on cancer initiation and progression. Cancer Sci. 99:1720–1725. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Gotte M and Yip GW: Heparanase, Hyaluronan, and CD44 in cancers: a breast carcinoma perspective. Cancer Res. 66:10233–10237. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Serra M, Rabanal RM, Miquel L, Domenzain C and Bassols A: Differential expression of CD44 in canine melanocytic tumours. J Comp Path. 130:171–180. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Paltian V, Alldinger S, Baumgartner W and Wohlsein P: Expression of CD44 in canine mammary tumours. J Comp Path. 141:237–247. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Nakamura T, Funahashi M, Takagaki K, Munakata H, Tanaka K, Saito Y and Endo M: Effect of 4-methylumbelliferone on cell-free synthesis of hyaluronic acid. Biochem Mol Biol Int. 43:263–268. 1997.PubMed/NCBI

26 

Kakizaki I, Kojima K, Takagaki K, Endo M, Kannagi R, Ito M, Maruo Y, Sato H, Yasuda T, Mita S, Kimata K and Itano N: A novel mechanism for the inhibition of hyaluronan biosynthesis by 4-Methylumbelliferone. J Biol Chem. 279:33281–33289. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Kultti A, Pasonen-Seppanen S, Jauhiainen M, Rilla KJ, Karna R, Pyoria E, Tammi RH and Tammi MI: 4-Methylumbelliferone inhibits hyaluronan synthesis by depletion of cellular UDP-glucuronic acid and downregulation of hyaluronan synthase 2 and 3. Exp Cell Res. 315:1914–1923. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Lokeshwar VB, Lopez LE, Munoz D, Chi A, Shirodkar SP, Lokeshwar SD, Escudero DO, Dhir N and Altman N: Antitumor activity of hyaluronic acid synthesis inhibitor 4-methylumbelliferone in prostate cancer cells. Cancer Res. 70:2613–2623. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Yoshihara S, Kon A, Kudo D, Nakazawa H, Kakizaki I, Sasaki M, Endo M and Takagaki K: A hyaluronan synthase suppressor, 4-methylumbelliferone, inhibits liver metastasis of melanoma cells. FEBS Lett. 579:2722–2726. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Knudson W and Knudson CB: Assembly of a chondrocyte-like pericellular matrix on non-chondrogenic cells. Role of the cell surface hyaluronan receptors in the assembly of a pericellular matrix. J Cell Sci. 99:227–235. 1991.PubMed/NCBI

31 

Hansen MB, Nielsen SE and Berg K: Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 119:203–210. 1989. View Article : Google Scholar : PubMed/NCBI

32 

Twentyman PR and Luscombe M: A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br J Cancer. 56:279–285. 1987. View Article : Google Scholar : PubMed/NCBI

33 

Saito T, Kawana H, Azuma K, Toyoda A, Fujita H, Kitagawa M and Harigaya K: Fragmented hyaluronan is an autocrine chemokinetic motility factor supported by the HAS2-HYAL2/CD44 system on the plasma membrane. Int J Oncol. 39:1311–1320. 2011.PubMed/NCBI

34 

Simpson M, Reiland J, Burger S, Furcht L, Spicer A, Oegema T and MaCarthy JB: Hyaluronan synthase elevation in metastatic prostate carcinoma cells correlates with hyaluronan surface retention, a prerequisite for rapid adhesion to bone marrow endothelial cells. J Biol Chem. 276:17949–17957. 2001. View Article : Google Scholar

35 

Li Y, Li L, Brown TJ and Heldin P: Silencing of hyaluronan synthase 2 suppresses the malignant phenotype of invasive breast cancer cells. Int J Cancer. 120:2557–2567. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Xia W, Spector S, Hardy L, Zhao S, Saluk A, Alemane L and Spector NL: Tumor selective G2/M cell cycle arrest and apoptosis of epithelial and hematological malignancies by BBL22, a benzazepine. Proc Natl Acad Sci USA. 97:7494–7499. 2000. View Article : Google Scholar : PubMed/NCBI

36 

Arai E, Nishida Y, Wasa J, Urakawa H, Zhuo L, Kimata K, Kozawa E, Futamura N and Ishiguro N: Inhibition of hyaluronan retention by 4-methylumbelliferone suppresses osteosarcoma cells in vitro and lung metastasis in vivo. Br J Cancer. 105:1839–1849. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Urakawa H, Nishida Y, Wasa J, Arai E, Zhuo L, Kimata K, Kozawa E, Futamura N and Ishiguro N: Inhibition of hyaluronan synthesis in breast cancer cells by 4-methylumbelliferone suppresses tumorigenicity in vitro and metastatic lesions of bone in vivo. Int J Cancer. 130:454–466. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Camenisch TD, Spicer AP, Brehm-Gibson T, Biesterfeldt J, Agustine ML, Calabro A Jr, Kubalak S, Klewer SE and McDonald JA: Disruption of hyaluronan synthase-2 abrogates normal cardiac morphogenesis and hyaluronan-mediated transformation of epithelium to mesenchyme. J Clin Invest. 106:349–360. 2000. View Article : Google Scholar : PubMed/NCBI

39 

Vigetti D, Genasetti A, Karousou E, Viola M, Moretto P, Clerici M, Deleonibus S, De Luca G, Hascall VC and Passi A: Proinflammatory cytokines induce hyaluronan synthesis and monocyte adhesion in human endothelial cells through hyaluronan synthase 2 (HAS2) and the nuclear factor-kappaB (NF-kappaB) pathway. J Biol Chem. 285:24639–24645. 2010. View Article : Google Scholar

40 

Itano N and Kimata K: Mammalian hyaluronan synthases. IUBMB Life. 54:195–199. 2002. View Article : Google Scholar

41 

Okuda H, Kobayashi A, Xia B, Watabe M, Pai SK, Hirota S, Xing F, Liu W, Pandey PR, Fukuda K, Modur V, Ghosh A, Wilber A and Watabe K: Hyaluronan synthase HAS2 promotes tumor progression in bone by stimulating the interaction of breast cancer stem-like cells with macrophages and stromal cells. Cancer Res. 72:537–547. 2012. View Article : Google Scholar : PubMed/NCBI

42 

Sironen RK, Tammi M, Tammi R, Auvinen PK, Anttila M and Kosma VM: Hyaluronan in human malignancies. Exp Cell Res. 317:383–391. 2011. View Article : Google Scholar : PubMed/NCBI

43 

Bourguignon LY, Zhu H, Chu A, Iida N, Zhang L and Hung MC: Interaction between the adhesion receptor, CD44, and the oncogene product, p185HER2, promotes human ovarian tumor cell activation. J Biol Chem. 272:27913–27918. 1997. View Article : Google Scholar : PubMed/NCBI

44 

Ouhit A, Abd Elmageed ZY, Abdraboh ME, Lioe TF and Raj MH: In vivo evidence for the role of CD44s in promoting breast cancer metastasis to the liver. Am J Pathol. 171:2033–2039. 2007. View Article : Google Scholar : PubMed/NCBI

45 

Sohara Y, Ishiguro N, Machida K, Kurata H, Thant AA, Senga T, Matsuda S, Kimata K, Iwata H and Hamaguchi M: Hyaluronan activates cell motility of v-Src-transformed cells via Ras-mitogen-activated protein kinase and phosphoinositide 3-kinase-Akt in a tumor-specific manner. Mol Biol Cell. 12:1859–1868. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Saito T, Tamura D, Nakamura T, Makita Y, Ariyama H, Komiyama K, Yoshihara T and Asano R: 4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells. Oncol Rep 29: 335-342, 2013.
APA
Saito, T., Tamura, D., Nakamura, T., Makita, Y., Ariyama, H., Komiyama, K. ... Asano, R. (2013). 4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells. Oncology Reports, 29, 335-342. https://doi.org/10.3892/or.2012.2100
MLA
Saito, T., Tamura, D., Nakamura, T., Makita, Y., Ariyama, H., Komiyama, K., Yoshihara, T., Asano, R."4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells". Oncology Reports 29.1 (2013): 335-342.
Chicago
Saito, T., Tamura, D., Nakamura, T., Makita, Y., Ariyama, H., Komiyama, K., Yoshihara, T., Asano, R."4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells". Oncology Reports 29, no. 1 (2013): 335-342. https://doi.org/10.3892/or.2012.2100
Copy and paste a formatted citation
x
Spandidos Publications style
Saito T, Tamura D, Nakamura T, Makita Y, Ariyama H, Komiyama K, Yoshihara T and Asano R: 4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells. Oncol Rep 29: 335-342, 2013.
APA
Saito, T., Tamura, D., Nakamura, T., Makita, Y., Ariyama, H., Komiyama, K. ... Asano, R. (2013). 4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells. Oncology Reports, 29, 335-342. https://doi.org/10.3892/or.2012.2100
MLA
Saito, T., Tamura, D., Nakamura, T., Makita, Y., Ariyama, H., Komiyama, K., Yoshihara, T., Asano, R."4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells". Oncology Reports 29.1 (2013): 335-342.
Chicago
Saito, T., Tamura, D., Nakamura, T., Makita, Y., Ariyama, H., Komiyama, K., Yoshihara, T., Asano, R."4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells". Oncology Reports 29, no. 1 (2013): 335-342. https://doi.org/10.3892/or.2012.2100
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