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Article

Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways

  • Authors:
    • Jue Ouyang
    • Minghua Wu
    • Chen Huang
    • Li Cao
    • Guiyuan Li
  • View Affiliations / Copyright

    Affiliations: Cancer Research Institute, Disease Genome Research Center, Central South University; Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education; Key Laboratory of Carcinogenesis, Ministry of Health, Changsha, Hunan, P.R. China, Medical Research Center, Peking University Third Hospital, Beijing, P.R. China
  • Pages: 79-86
    |
    Published online on: October 23, 2012
       https://doi.org/10.3892/or.2012.2101
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Abstract

Oxidored-nitro domain containing protein 1 (NOR1) is a novel member of the nitroreductase family that was first isolated as a tumor suppressor gene from human nasopharyngeal carcinoma (NPC). However, the role of NOR1 gene dysfunction in human cancers has not been addressed. We analyzed the expression of NOR1 in various human cancer and benign tissue specimens and found significant downregulation in nine types of cancer compared with corresponding non-tumor tissues. The recombinant expression vector pCDNA3.1-myc-his-NOR1 was constructed and transfected into human NPC 6-10B nasopharyngeal cancer and HeLa cervical cancer (CCA) cells. We found that stable NOR1 overexpression resulted in suppression of 6-10B and HeLa cell proliferation and led to S phase cell cycle arrest. In addition, NOR1 upregulation enhanced apoptosis in pCDNA3.1-myc-his-NOR1 stably transfected cells, and it also altered the expression of proteins involved in the mitochondria-dependent apoptotic pathway. Furthermore, we also found that the NOR1 protein is a cytoplasmic protein that is partially localized in the mitochondria and endoplasmic reticulum. Therefore, NOR1 is an important tumor suppressor gene associated with NPC and CCA and may play antitumor roles by inhibiting proliferation, preventing colony formation, and promoting the apoptosis of tumor cells via the mitochondrial-dependent apoptotic pathway. However, the precise mechanism behind the NOR1 antitumor effects needs to be investigated further.
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Copy and paste a formatted citation
Spandidos Publications style
Ouyang J, Wu M, Huang C, Cao L and Li G: Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways. Oncol Rep 29: 79-86, 2013.
APA
Ouyang, J., Wu, M., Huang, C., Cao, L., & Li, G. (2013). Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways. Oncology Reports, 29, 79-86. https://doi.org/10.3892/or.2012.2101
MLA
Ouyang, J., Wu, M., Huang, C., Cao, L., Li, G."Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways". Oncology Reports 29.1 (2013): 79-86.
Chicago
Ouyang, J., Wu, M., Huang, C., Cao, L., Li, G."Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways". Oncology Reports 29, no. 1 (2013): 79-86. https://doi.org/10.3892/or.2012.2101
Copy and paste a formatted citation
x
Spandidos Publications style
Ouyang J, Wu M, Huang C, Cao L and Li G: Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways. Oncol Rep 29: 79-86, 2013.
APA
Ouyang, J., Wu, M., Huang, C., Cao, L., & Li, G. (2013). Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways. Oncology Reports, 29, 79-86. https://doi.org/10.3892/or.2012.2101
MLA
Ouyang, J., Wu, M., Huang, C., Cao, L., Li, G."Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways". Oncology Reports 29.1 (2013): 79-86.
Chicago
Ouyang, J., Wu, M., Huang, C., Cao, L., Li, G."Overexpression of oxidored-nitro domain containing protein 1 inhibits human nasopharyngeal carcinoma and cervical cancer cell proliferation and induces apoptosis: Involvement of mitochondrial apoptotic pathways". Oncology Reports 29, no. 1 (2013): 79-86. https://doi.org/10.3892/or.2012.2101
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