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Article

Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation

  • Authors:
    • Ruifan Xie
    • Hai Yang
    • Qungen Xiao
    • Feng Mao
    • Suojun Zhang
    • Fei Ye
    • Feng Wan
    • Baofeng Wang
    • Ting Lei
    • Dongsheng Guo
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery and Sino-German Neuro-Oncology Molecular Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
  • Pages: 177-184
    |
    Published online on: October 23, 2012
       https://doi.org/10.3892/or.2012.2102
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Abstract

The LRIG1 [leucine-rich repeats and immunoglobulin-like domains (LRIG)] gene is not universally downregulated in human cancers, and its role in tumorigenesis and the development of glioma has not been well addressed. In this study, we used short hairpin RNA (shRNA)-triggered RNA interference (RNAi) to block LRIG1 gene expression in the GL15 human glioma cell line. Specific downregulation of LRIG1 by shRNA resulted in significantly enhanced capabilities of proliferation, inhibition of apoptosis and invasion in the GL15 cells. LRIG1 repression induced marked activation of epidermal growth factor receptor (EGFR), protein kinase B (Akt) and c-Myc signaling molecules. Our results demonstrated that RNAi against LRIG1 may effectively downregulate LRIG1 gene expression. LRIG1 functions as a tumor suppressor in the pathogenesis of glioma via EGFR/Akt/c-Myc activation.
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Copy and paste a formatted citation
Spandidos Publications style
Xie R, Yang H, Xiao Q, Mao F, Zhang S, Ye F, Wan F, Wang B, Lei T, Guo D, Guo D, et al: Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation. Oncol Rep 29: 177-184, 2013.
APA
Xie, R., Yang, H., Xiao, Q., Mao, F., Zhang, S., Ye, F. ... Guo, D. (2013). Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation. Oncology Reports, 29, 177-184. https://doi.org/10.3892/or.2012.2102
MLA
Xie, R., Yang, H., Xiao, Q., Mao, F., Zhang, S., Ye, F., Wan, F., Wang, B., Lei, T., Guo, D."Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation". Oncology Reports 29.1 (2013): 177-184.
Chicago
Xie, R., Yang, H., Xiao, Q., Mao, F., Zhang, S., Ye, F., Wan, F., Wang, B., Lei, T., Guo, D."Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation". Oncology Reports 29, no. 1 (2013): 177-184. https://doi.org/10.3892/or.2012.2102
Copy and paste a formatted citation
x
Spandidos Publications style
Xie R, Yang H, Xiao Q, Mao F, Zhang S, Ye F, Wan F, Wang B, Lei T, Guo D, Guo D, et al: Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation. Oncol Rep 29: 177-184, 2013.
APA
Xie, R., Yang, H., Xiao, Q., Mao, F., Zhang, S., Ye, F. ... Guo, D. (2013). Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation. Oncology Reports, 29, 177-184. https://doi.org/10.3892/or.2012.2102
MLA
Xie, R., Yang, H., Xiao, Q., Mao, F., Zhang, S., Ye, F., Wan, F., Wang, B., Lei, T., Guo, D."Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation". Oncology Reports 29.1 (2013): 177-184.
Chicago
Xie, R., Yang, H., Xiao, Q., Mao, F., Zhang, S., Ye, F., Wan, F., Wang, B., Lei, T., Guo, D."Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation". Oncology Reports 29, no. 1 (2013): 177-184. https://doi.org/10.3892/or.2012.2102
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