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Article

INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours

  • Authors:
    • Thatchawan Thanasupawat
    • Katrin Hammje
    • Ibrahim Adham
    • Jean-Eric Ghia
    • Marc R. Del Bigio
    • Jerry Krcek
    • Cuong Hoang-Vu
    • Thomas Klonisch
    • Sabine Hombach-Klonisch
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy and Cell Science, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 0J9, Canada, Clinics of General, Visceral and Vascular Surgery, Martin Luther University (MLU), Halle-Wittenberg, Germany, Institute of Human Genetics, University of Göttingen, Göttingen, Germany, Department of Internal Medicine, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 0J9, Canada
  • Pages: 149-154
    |
    Published online on: October 31, 2012
       https://doi.org/10.3892/or.2012.2119
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Abstract

We report for the first time the distribution of human INSL5 and its cognate leucine rich G-protein coupled receptor RXFP4 in the large intestine and in neuroendocrine/carcinoid tissues. Immunoreactive INSL5 was uniquely expressed by enteroendocrine cells (EECs) located within the colonic mucosa, whereas colonocytes were immunopositive for RXFP4. INSL5+ and RXFP4+ cells were also detected in human neuroendocrine/carcinoid tissues. We employed a recently described Insl5 knockout mouse model and 2 mouse models of induced colitis to address the relevance of Insl5 in EEC development and in acute inflammation of the colon. We identified INSL5 as a specific marker for synaptophysin+ EECs in the mucosa of the normal human and mouse colon. Insl5 was not essential for the development of mouse synaptophysin+ EECs. The mouse models of chemically induced colitis (dextran sulfate sodium and dinitrobenzene-sulfonic acid) failed to show changes in the numbers of Insl5+ EECs at inflammatory sites during the acute phase of colitis. In conclusion, we showed that INSL5 is a novel marker of colorectal EECs and provide first evidence for the presence of a potentially autocrine/paracrine INSL5-RXFP4 signaling system in the normal human and mouse colon and in rare human neuroendocrine tumours.
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Copy and paste a formatted citation
Spandidos Publications style
Thanasupawat T, Hammje K, Adham I, Ghia J, Del Bigio MR, Krcek J, Hoang-Vu C, Klonisch T and Hombach-Klonisch S: INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours. Oncol Rep 29: 149-154, 2013.
APA
Thanasupawat, T., Hammje, K., Adham, I., Ghia, J., Del Bigio, M.R., Krcek, J. ... Hombach-Klonisch, S. (2013). INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours. Oncology Reports, 29, 149-154. https://doi.org/10.3892/or.2012.2119
MLA
Thanasupawat, T., Hammje, K., Adham, I., Ghia, J., Del Bigio, M. R., Krcek, J., Hoang-Vu, C., Klonisch, T., Hombach-Klonisch, S."INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours". Oncology Reports 29.1 (2013): 149-154.
Chicago
Thanasupawat, T., Hammje, K., Adham, I., Ghia, J., Del Bigio, M. R., Krcek, J., Hoang-Vu, C., Klonisch, T., Hombach-Klonisch, S."INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours". Oncology Reports 29, no. 1 (2013): 149-154. https://doi.org/10.3892/or.2012.2119
Copy and paste a formatted citation
x
Spandidos Publications style
Thanasupawat T, Hammje K, Adham I, Ghia J, Del Bigio MR, Krcek J, Hoang-Vu C, Klonisch T and Hombach-Klonisch S: INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours. Oncol Rep 29: 149-154, 2013.
APA
Thanasupawat, T., Hammje, K., Adham, I., Ghia, J., Del Bigio, M.R., Krcek, J. ... Hombach-Klonisch, S. (2013). INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours. Oncology Reports, 29, 149-154. https://doi.org/10.3892/or.2012.2119
MLA
Thanasupawat, T., Hammje, K., Adham, I., Ghia, J., Del Bigio, M. R., Krcek, J., Hoang-Vu, C., Klonisch, T., Hombach-Klonisch, S."INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours". Oncology Reports 29.1 (2013): 149-154.
Chicago
Thanasupawat, T., Hammje, K., Adham, I., Ghia, J., Del Bigio, M. R., Krcek, J., Hoang-Vu, C., Klonisch, T., Hombach-Klonisch, S."INSL5 is a novel marker for human enteroendocrine cells of the large intestine and neuroendocrine tumours". Oncology Reports 29, no. 1 (2013): 149-154. https://doi.org/10.3892/or.2012.2119
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