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Article

Early growth response 1 regulates glucose deprivation-induced necrosis

  • Authors:
    • Hyun Min Jeon
    • Su Yeon Lee
    • Min Kyung Ju
    • Cho Hee Kim
    • Hye Gyeong Park
    • Ho Sung Kang
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735, Republic of Korea, Nanobiotechnology Center, Pusan National University, Pusan 609-735, Republic of Korea
  • Pages: 669-675
    |
    Published online on: November 14, 2012
       https://doi.org/10.3892/or.2012.2134
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Abstract

Necrosis is commonly found in the core region of solid tumours due to metabolic stress such as hypoxia and glucose deprivation (GD) resulting from insufficient vascularization. Necrosis promotes tumour growth and development by releasing the tumour-promoting cytokine high mobility group box 1 (HMGB1); however, the molecular mechanism underlying necrotic cell death remains largely unknown. In this study, we show that early growth response 1 (Egr-1) is induced in a reactive oxygen species (ROS)-dependent manner by GD in several cell lines such as A549, MDA-MB-231 and HepG2 cells that exhibit necrosis upon GD. We found that Egr-1 short hairpin RNA (shRNA) prevented GD-induced necrosis and HMGB1 release. Necrosis-inhibiting activity of Egr-1 shRNA was also seen in multicellular tumour spheroids (MTSs), an in vitro tumour model system. In contrast, Egr-1 overexpression appeared to make tumour cells more susceptible to GD-induced necrosis. Finally, Egr-1 shRNA suppressed the growth of MTSs. These findings demonstrate that Egr-1 is implicated in GD-induced necrosis and tumour progression.
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Copy and paste a formatted citation
Spandidos Publications style
Jeon HM, Lee SY, Ju MK, Kim CH, Park HG and Kang HS: Early growth response 1 regulates glucose deprivation-induced necrosis. Oncol Rep 29: 669-675, 2013.
APA
Jeon, H.M., Lee, S.Y., Ju, M.K., Kim, C.H., Park, H.G., & Kang, H.S. (2013). Early growth response 1 regulates glucose deprivation-induced necrosis. Oncology Reports, 29, 669-675. https://doi.org/10.3892/or.2012.2134
MLA
Jeon, H. M., Lee, S. Y., Ju, M. K., Kim, C. H., Park, H. G., Kang, H. S."Early growth response 1 regulates glucose deprivation-induced necrosis". Oncology Reports 29.2 (2013): 669-675.
Chicago
Jeon, H. M., Lee, S. Y., Ju, M. K., Kim, C. H., Park, H. G., Kang, H. S."Early growth response 1 regulates glucose deprivation-induced necrosis". Oncology Reports 29, no. 2 (2013): 669-675. https://doi.org/10.3892/or.2012.2134
Copy and paste a formatted citation
x
Spandidos Publications style
Jeon HM, Lee SY, Ju MK, Kim CH, Park HG and Kang HS: Early growth response 1 regulates glucose deprivation-induced necrosis. Oncol Rep 29: 669-675, 2013.
APA
Jeon, H.M., Lee, S.Y., Ju, M.K., Kim, C.H., Park, H.G., & Kang, H.S. (2013). Early growth response 1 regulates glucose deprivation-induced necrosis. Oncology Reports, 29, 669-675. https://doi.org/10.3892/or.2012.2134
MLA
Jeon, H. M., Lee, S. Y., Ju, M. K., Kim, C. H., Park, H. G., Kang, H. S."Early growth response 1 regulates glucose deprivation-induced necrosis". Oncology Reports 29.2 (2013): 669-675.
Chicago
Jeon, H. M., Lee, S. Y., Ju, M. K., Kim, C. H., Park, H. G., Kang, H. S."Early growth response 1 regulates glucose deprivation-induced necrosis". Oncology Reports 29, no. 2 (2013): 669-675. https://doi.org/10.3892/or.2012.2134
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