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Article

Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells

  • Authors:
    • Eun Jeong Gweon
    • Song Ja Kim
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, College of Natural Sciences, Kongju National University, Gongju, Chungnam 314-701, Republic of Korea
  • Pages: 826-834
    |
    Published online on: November 27, 2012
       https://doi.org/10.3892/or.2012.2151
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Abstract

Trans-3,4',5-trihydroxystilbene (resveratrol) is a grape polyphenol present in various plants, food products, red wine and grapes. Resveratrol has anti-inflammatory, anticarcinogenic, anti-oxidant and anti-aging properties. Matrix metalloproteinases (MMPs) are key enzymes involved in the degradation of the extracellular matrix, and their expression may be regulated in cancer metastasis. In the present study, we aimed to evaluate the effect of resveratrol on MMPs and cell migration, and to understand the mechanism of action in HT1080 human fibrosarcoma cells. We found that resveratrol inhibited HT1080 cell viability at various concentrations as detected by the MTT assay and FACS analysis. However, resveratrol dramatically increased the activation and expression of MMP-9 in a dose- and time-dependent manner, as determined by gelatin zymography assay and western blot analysis. We also discovered that resveratrol enhanced the migratory ability of HT1080 cells, as determined by the wound healing assay, and decreased the phosphorylation of p38 kinase. Moreover, the Akt kinase was inhibited by resveratrol in the HT1080 cells. The inhibition of p38 and Akt kinases with SB203580 and LY294002 further increased resveratrol-induced MMP-9 as well as cell migration in the HT1080 cells. Our results suggest that resveratrol regulates MMP-9 and migratory abilities through the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Gweon EJ and Kim SJ: Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells. Oncol Rep 29: 826-834, 2013.
APA
Gweon, E.J., & Kim, S.J. (2013). Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells. Oncology Reports, 29, 826-834. https://doi.org/10.3892/or.2012.2151
MLA
Gweon, E. J., Kim, S. J."Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells". Oncology Reports 29.2 (2013): 826-834.
Chicago
Gweon, E. J., Kim, S. J."Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells". Oncology Reports 29, no. 2 (2013): 826-834. https://doi.org/10.3892/or.2012.2151
Copy and paste a formatted citation
x
Spandidos Publications style
Gweon EJ and Kim SJ: Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells. Oncol Rep 29: 826-834, 2013.
APA
Gweon, E.J., & Kim, S.J. (2013). Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells. Oncology Reports, 29, 826-834. https://doi.org/10.3892/or.2012.2151
MLA
Gweon, E. J., Kim, S. J."Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells". Oncology Reports 29.2 (2013): 826-834.
Chicago
Gweon, E. J., Kim, S. J."Resveratrol induces MMP-9 and cell migration via the p38 kinase and PI-3K pathways in HT1080 human fibrosarcoma cells". Oncology Reports 29, no. 2 (2013): 826-834. https://doi.org/10.3892/or.2012.2151
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