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Article

Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features

  • Authors:
    • Yifeng Sun
    • Bo Su
    • Peng Zhang
    • Huikang Xie
    • Hui Zheng
    • Yongjie Xu
    • Qiaoling Du
    • Huan Zeng
    • Xiao Zhou
    • Chang Chen
    • Wen Gao
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, P.R. China, Department of Central Laboratory, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, P.R. China, Department of Pathology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, P.R. China, Department of Gynaecology and Obstetrics, Shanghai First Maternity and Infant Health Hospital, Tongji University School of Medicine, Shanghai 200126, P.R. China, Department of Thoracic Surgery, Shanghai Chest Hospital Affiliated with Shanghai Jiaotong University, Shanghai 200030, P.R. China
  • Pages: 704-712
    |
    Published online on: November 28, 2012
       https://doi.org/10.3892/or.2012.2152
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Abstract

The present study investigated the expression of miR-150 and miR-3940-5p in non-small cell lung carcinoma (NSCLC) and its relationship with clinicopathologic features. Samples included tumor, tumor-adjacent and normal lung parenchyma tissues from 90 NSCLC patients and 17 cases of embryonic lung cDNA. The expression levels of miR-150, miR-18b-5p, miR-643 and miR-3940-5p were detected by real‑time PCR; p53, EGFR, Kras and Ki-67 expression in tumor tissues was determined by immunohistochemistry. p53 mRNA expression levels in NSCLC were examined by SYBR-Green real-time PCR. The relationship between the four miRNAs and clinicopathologic features of 90 cases was analyzed. The expression of miR-150 and miR-3940-5p was significantly downregulated in tumor tissues and embryonic lung tissues compared to normal lung tissues. The expression of miR-150 and miR-3940-5p in tumor tissues was also lower than that in the matched tumor-adjacent tissues. miR-150 was downregulated preferentially in subgroups of patients with a tumor diameter more than or equal to 3 cm, in smokers and in stage III and IV tumors. Specifically, miR-150 and miR-3940-5p expression was decreased in nuclear cell proliferation antigen Ki-67-positive NSCLC cases. miR-150 and miR-3940-5p were found to be significantly downregulated in p53 IHC-positive NSCLC cases and were negatively correlated with p53 mRNA. Reduced miR-150 and miR-3940-5p expression in tumor tissues and embryonic lung tissues suggests that these miRs may be involved in the tumorigenesis or de-differentiation of NSCLC. Due to this associaton with the Ki-67 proliferation index in NSCLC, downregulation of miR-150 and miR-3940-5p may contribute to tumor growth and proliferation. miR-150 and miR-3940-5p may affect p53 expression through a direct or indirect pathway.
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1 

Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Eder M and Scherr M: MicroRNA and lung cancer. N Engl J Med. 352:2446–2448. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Scott GK, Goga A, Bhaumik D, Berger CE, Sullivan CS and Benz CC: Coordinate suppression of ERBB2 and ERBB3 by enforced expression of micro-RNA miR-125a or miR-125b. J Biol Chem. 282:1479–1486. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Jemal A, Siegel R, Ward E, Murray T, Xu J and Thun MJ: Cancer statistics, 2007. CA Cancer J Clin. 57:43–66. 2007. View Article : Google Scholar

6 

Goldstraw P, Crowley J, Chansky K, et al: The IASLC Lung Cancer Staging Project: proposals for the revision of the TNM stage groupings in the forthcoming (seventh) edition of the TNM Classification of Malignant Tumours. J Thorac Oncol. 2:706–714. 2007. View Article : Google Scholar

7 

Govindan R, Page N, Morgensztern D, et al: Changing epidemiology of small-cell lung cancer in the United States over the last 30 years: analysis of the surveillance, epidemiologic, and end results database. J Clin Oncol. 24:4539–4544. 2006.PubMed/NCBI

8 

Takamizawa J, Konishi H, Yanagisawa K, et al: Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res. 64:3753–3756. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Yu SL, Chen HY, Chang GC, et al: MicroRNA signature predicts survival and relapse in lung cancer. Cancer Cell. 13:48–57. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Raponi M, Dossey L, Jatkoe T, et al: MicroRNA classifiers for predicting prognosis of squamous cell lung cancer. Cancer Res. 69:5776–5783. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Soomro IN, Holmes J and Whimster WF: Predicting prognosis in lung cancer: use of proliferation marker, Ki67 monoclonal antibody. J Pak Med Assoc. 48:66–69. 1998.PubMed/NCBI

12 

Tiscornia G and Izpisua Belmonte JC: MicroRNAs in embryonic stem cell function and fate. Genes Dev. 24:2732–2741. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Monzo M, Navarro A, Bandres E, et al: Overlapping expression of microRNAs in human embryonic colon and colorectal cancer. Cell Res. 18:823–833. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Scholzen T and Gerdes J: The Ki-67 protein: from the known and the unknown. J Cell Physiol. 182:311–322. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Takahashi S, Kamata Y, Tamo W, et al: Relationship between postoperative recurrence and expression of cyclin E, p27, and Ki-67 in non-small cell lung cancer without lymph node metastases. Int J Clin Oncol. 7:349–355. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Martin B, Paesmans M, Mascaux C, et al: Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis. Br J Cancer. 91:2018–2025. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Woo T, Okudela K, Yazawa T, et al: Prognostic value of KRAS mutations and Ki-67 expression in stage I lung adenocarcinomas. Lung Cancer. 65:355–362. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Watanabe A, Tagawa H, Yamashita J, et al: The role of microRNA-150 as a tumor suppressor in malignant lymphoma. Leukemia. 25:1324–1334. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Ma Y, Zhang P, Wang F, et al: miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer. Gut. 61:1447–1453. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Toyooka S, Matsuo K, Shigematsu H, et al: The impact of sex and smoking status on the mutational spectrum of epidermal growth factor receptor gene in non-small cell lung cancer. Clin Cancer Res. 13:5763–5768. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Izzotti A, Calin GA, Arrigo P, Steele VE, Croce CM and De Flora S: Downregulation of microRNA expression in the lungs of rats exposed to cigarette smoke. FASEB J. 23:806–812. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Liao JY, Ma LM, Guo YH, et al: Deep sequencing of human nuclear and cytoplasmic small RNAs reveals an unexpectedly complex subcellular distribution of miRNAs and tRNA 3′ trailers. PLoS One. 5:e105632010.PubMed/NCBI

23 

Persson H, Kvist A, Rego N, et al: Identification of new microRNAs in paired normal and tumor breast tissue suggests a dual role for the ERBB2/Her2 gene. Cancer Res. 71:78–86. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Starzynska T, Bromley M, Ghosh A and Stern PL: Prognostic significance of p53 overexpression in gastric and colorectal carcinoma. Br J Cancer. 66:558–562. 1992. View Article : Google Scholar : PubMed/NCBI

25 

Andersen TI, Holm R, Nesland JM, Heimdal KR, Ottestad L and Borresen AL: Prognostic significance of TP53 alterations in breast carcinoma. Br J Cancer. 68:540–548. 1993. View Article : Google Scholar : PubMed/NCBI

26 

Chava S, Mohan V, Shetty PJ, et al: Immunohistochemical evaluation of p53, FHIT, and IGF2 gene expression in esophageal cancer. Dis Esophagus. 25:81–87. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Yamakuchi M, Ferlito M and Lowenstein CJ: miR-34a repression of SIRT1 regulates apoptosis. Proc Natl Acad Sci USA. 105:13421–13426. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Park SY, Lee JH, Ha M, Nam JW and Kim VN: miR-29 miRNAs activate p53 by targeting p85 alpha and CDC42. Nat Struct Mol Biol. 16:23–29. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Zhou BP, Liao Y, Xia W, Zou Y, Spohn B and Hung MC: HER-2/neu induces p53 ubiquitination via Akt-mediated MDM2 phosphorylation. Nat Cell Biol. 3:973–982. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Fornari F, Gramantieri L, Giovannini C, et al: MiR-122/cyclin G1 interaction modulates p53 activity and affects doxorubicin sensitivity of human hepatocarcinoma cells. Cancer Res. 69:5761–5767. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Laurent LC, Chen J, Ulitsky I, et al: Comprehensive microRNA profiling reveals a unique human embryonic stem cell signature dominated by a single seed sequence. Stem Cells. 26:1506–1516. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Ren J, Jin P, Wang E, Marincola FM and Stroncek DF: MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells. J Transl Med. 7:202009. View Article : Google Scholar : PubMed/NCBI

33 

Zhao C, Sun G, Li S, et al: MicroRNA let-7b regulates neural stem cell proliferation and differentiation by targeting nuclear receptor TLX signaling. Proc Natl Acad Sci USA. 107:1876–1881. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Melton C, Judson RL and Blelloch R: Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature. 463:621–626. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Yu F, Yao H, Zhu P, et al: let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell. 131:1109–1123. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun Y, Su B, Zhang P, Xie H, Zheng H, Xu Y, Du Q, Zeng H, Zhou X, Chen C, Chen C, et al: Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features. Oncol Rep 29: 704-712, 2013.
APA
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y. ... Gao, W. (2013). Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features. Oncology Reports, 29, 704-712. https://doi.org/10.3892/or.2012.2152
MLA
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y., Du, Q., Zeng, H., Zhou, X., Chen, C., Gao, W."Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features". Oncology Reports 29.2 (2013): 704-712.
Chicago
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y., Du, Q., Zeng, H., Zhou, X., Chen, C., Gao, W."Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features". Oncology Reports 29, no. 2 (2013): 704-712. https://doi.org/10.3892/or.2012.2152
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Y, Su B, Zhang P, Xie H, Zheng H, Xu Y, Du Q, Zeng H, Zhou X, Chen C, Chen C, et al: Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features. Oncol Rep 29: 704-712, 2013.
APA
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y. ... Gao, W. (2013). Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features. Oncology Reports, 29, 704-712. https://doi.org/10.3892/or.2012.2152
MLA
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y., Du, Q., Zeng, H., Zhou, X., Chen, C., Gao, W."Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features". Oncology Reports 29.2 (2013): 704-712.
Chicago
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y., Du, Q., Zeng, H., Zhou, X., Chen, C., Gao, W."Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features". Oncology Reports 29, no. 2 (2013): 704-712. https://doi.org/10.3892/or.2012.2152
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