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Article

Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model

  • Authors:
    • Ji-Ye Kee
    • Aya Ito
    • Shozo Hojo
    • Isaya Hashimoto
    • Yoshiko  Igarashi
    • Kazuhiro  Tsukada
    • Tatsuro Irimura
    • Naotoshi Shibahara
    • Takashi  Nakayama
    • Osamu  Yoshie
    • Hiroaki Sakurai
    • Ikuo Saiki
    • Keiichi Koizumi
  • View Affiliations / Copyright

    Affiliations: Division of Pathogenic Biochemistry, University of Toyama, Toyama 930-0194, Japan, Department of Surgery (II), Faculty of Medicine, University of Toyama, Toyama 930-0194, Japan, Division of Kampo Diagnostics, Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan, Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan, Division of Chemotherapy, Kinki University School of Pharmaceutical Sciences, Osaka 577-8502, Japan, Department of Microbiology, Kinki University School of Medicine, Osaka 589-8511, Japan, Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan
  • Pages: 975-982
    |
    Published online on: December 13, 2012
       https://doi.org/10.3892/or.2012.2185
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Abstract

Colorectal cancer (CRC) is a typical lifestyle-related disease, and it metastasizes mostly to the liver. It is important to understand the molecular mechanisms of CRC metastasis in order to design new and effective treatments for CRC patients. Chemokines are known to have antitumor effects as their chemoattractant properties stimulate the accumulation of infiltrating immune cells (TILs) in tumors. Chemokine (C-X-C motif) ligand 16 (CXCL16), also known as SR-PSOX, is a unique membrane-bound chemokine that induces the expression of its specific receptor CXCR6. We previously reported that the expression of CXCL16 by cancer cells enhances the recruitment of TILs, thereby improving the prognosis of CRC. It has since been reported that CXCL16/CXCR6 expression is involved in the metastasis of various types of cancer. However, there is no report of the association between CXCL16 expression and liver metastasis in CRC. In this study, we investigated the role of cancer-derived CXCL16 and the possibility of gene therapy using CXCL16. Therefore, we examined the metastasis of colon 38 SL4 cells to the liver in an experimental model. Following injection of cancer cells into the intraportal vein, CXCL16-expressing CRC cells drastically inhibited liver metastasis. We also found that CD8 T cells and natural killer T (NKT) cells, known as CXCR6-expressing cells, increased in CXCL16-expressing metastatic tissue. Collectively, the inhibitory effect on metastasis to the liver by CXCL16 was observed in NKT cell-depleted mice but not in CD8 T cell-depleted mice. These results demonstrate the inhibitory effect of CXCL16 on liver metastasis via NKT cells in CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Kee J, Ito A, Hojo S, Hashimoto I, Igarashi Y, Tsukada K, Irimura T, Shibahara N, Nakayama T, Yoshie O, Yoshie O, et al: Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model. Oncol Rep 29: 975-982, 2013.
APA
Kee, J., Ito, A., Hojo, S., Hashimoto, I., Igarashi, Y., Tsukada, K. ... Koizumi, K. (2013). Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model. Oncology Reports, 29, 975-982. https://doi.org/10.3892/or.2012.2185
MLA
Kee, J., Ito, A., Hojo, S., Hashimoto, I., Igarashi, Y., Tsukada, K., Irimura, T., Shibahara, N., Nakayama, T., Yoshie, O., Sakurai, H., Saiki, I., Koizumi, K."Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model". Oncology Reports 29.3 (2013): 975-982.
Chicago
Kee, J., Ito, A., Hojo, S., Hashimoto, I., Igarashi, Y., Tsukada, K., Irimura, T., Shibahara, N., Nakayama, T., Yoshie, O., Sakurai, H., Saiki, I., Koizumi, K."Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model". Oncology Reports 29, no. 3 (2013): 975-982. https://doi.org/10.3892/or.2012.2185
Copy and paste a formatted citation
x
Spandidos Publications style
Kee J, Ito A, Hojo S, Hashimoto I, Igarashi Y, Tsukada K, Irimura T, Shibahara N, Nakayama T, Yoshie O, Yoshie O, et al: Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model. Oncol Rep 29: 975-982, 2013.
APA
Kee, J., Ito, A., Hojo, S., Hashimoto, I., Igarashi, Y., Tsukada, K. ... Koizumi, K. (2013). Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model. Oncology Reports, 29, 975-982. https://doi.org/10.3892/or.2012.2185
MLA
Kee, J., Ito, A., Hojo, S., Hashimoto, I., Igarashi, Y., Tsukada, K., Irimura, T., Shibahara, N., Nakayama, T., Yoshie, O., Sakurai, H., Saiki, I., Koizumi, K."Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model". Oncology Reports 29.3 (2013): 975-982.
Chicago
Kee, J., Ito, A., Hojo, S., Hashimoto, I., Igarashi, Y., Tsukada, K., Irimura, T., Shibahara, N., Nakayama, T., Yoshie, O., Sakurai, H., Saiki, I., Koizumi, K."Chemokine CXCL16 suppresses liver metastasis of colorectal cancer via augmentation of tumor-infiltrating natural killer T cells in a murine model". Oncology Reports 29, no. 3 (2013): 975-982. https://doi.org/10.3892/or.2012.2185
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