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Article

Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration

  • Authors:
    • Zijian Yang
    • Jing Wang
    • Xiaohong Liu
    • Yu Cheng
    • Lianfu Deng
    • Yisheng Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200025, P.R. China, Shanghai Institute of Traumatology and Orthopedics, Shanghai 200025, P.R. China
  • Pages: 1140-1146
    |
    Published online on: December 20, 2012
       https://doi.org/10.3892/or.2012.2205
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Abstract

Y-39983, a selective Rho-associated kinase (ROCK) inhibitor, promotes axonal regeneration of damaged retinal ganglion cells (RGCs). The present study investigated the effects of Y-39983 on RhoA/ROCK expression during promotion of axonal regeneration using a rat optic nerve crush (ONC) model. Herein, we demonstrated that Y-39983 significantly enhanced the survival and axonal regeneration of RGCs after ONC. Using a pull‑down assay and affinity precipitation to examine the activity of RhoA, we detected the decreased expression of active-RhoA after using Y-39983. The expression of ROCK1 and ROCK2 was significantly decreased as demonstrated by RT-PCR, immunohistochemistry and western blot analysis. The downregulation of active-RhoA, ROCK1 and ROCK2 expression by Y-39983 coincided with the appearance of larger numbers of regenerating axons. In conclusion, Y-39983 downregulated the expression of active-RhoA, ROCK1 and ROCK2 during its promotion of axonal regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Wang J, Liu X, Cheng Y, Deng L and Zhong Y: Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration . Oncol Rep 29: 1140-1146, 2013.
APA
Yang, Z., Wang, J., Liu, X., Cheng, Y., Deng, L., & Zhong, Y. (2013). Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration . Oncology Reports, 29, 1140-1146. https://doi.org/10.3892/or.2012.2205
MLA
Yang, Z., Wang, J., Liu, X., Cheng, Y., Deng, L., Zhong, Y."Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration ". Oncology Reports 29.3 (2013): 1140-1146.
Chicago
Yang, Z., Wang, J., Liu, X., Cheng, Y., Deng, L., Zhong, Y."Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration ". Oncology Reports 29, no. 3 (2013): 1140-1146. https://doi.org/10.3892/or.2012.2205
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Wang J, Liu X, Cheng Y, Deng L and Zhong Y: Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration . Oncol Rep 29: 1140-1146, 2013.
APA
Yang, Z., Wang, J., Liu, X., Cheng, Y., Deng, L., & Zhong, Y. (2013). Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration . Oncology Reports, 29, 1140-1146. https://doi.org/10.3892/or.2012.2205
MLA
Yang, Z., Wang, J., Liu, X., Cheng, Y., Deng, L., Zhong, Y."Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration ". Oncology Reports 29.3 (2013): 1140-1146.
Chicago
Yang, Z., Wang, J., Liu, X., Cheng, Y., Deng, L., Zhong, Y."Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration ". Oncology Reports 29, no. 3 (2013): 1140-1146. https://doi.org/10.3892/or.2012.2205
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