Open Access

Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure

  • Authors:
    • Hyung Tae Lee
    • Se Kye Kim
    • Mi Ran Choi
    • Ji Hyun Park
    • Kyoung Hwa Jung
    • Young Gyu Chai
  • View Affiliations

  • Published online on: December 24, 2012     https://doi.org/10.3892/or.2012.2209
  • Pages: 868-874
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Compared to other cancers affecting women, breast cancer is significantly associated with alcohol consumption. However, the principles underlying the carcinogenesis of alcohol-induced breast cancer and the related metastatic mechanisms have yet to be established. To observe the effect of alcohol on the growth regulation in breast cancer cells, we identified differentially expressed proteins in alcohol-exposed human breast cancer T47D cells using gel-based proteomics analysis. The expression of c-ros receptor tyrosine kinase (ROS1) was increased and activated by autophosphorylation, thereby activating mitogen- and stress-activated protein kinase 1 (MSK1) through the mitogen‑activated protein kinase (MAPK) pathway; activated MSK1, in turn, phosphorylated histone 3 serine 10 (H3S10p) residues in the nucleus. The increase in H3S10 phosphorylation consequently increased the level of expression of immediate-early gene such as c-fos. This study demonstrated that when breast cancer cells are exposed to alcohol, phosphorylated ROS1 activates MSK1 via Erk1/2 in the MAPK pathway, which then induces modifications to histone residues that regulate gene expression by 14-3-3 protein recruitment, leading to a lack of control of breast cancer cell proliferation.
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March 2013
Volume 29 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lee HT, Kim SK, Choi MR, Park JH, Jung KH and Chai YG: Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure. Oncol Rep 29: 868-874, 2013
APA
Lee, H.T., Kim, S.K., Choi, M.R., Park, J.H., Jung, K.H., & Chai, Y.G. (2013). Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure. Oncology Reports, 29, 868-874. https://doi.org/10.3892/or.2012.2209
MLA
Lee, H. T., Kim, S. K., Choi, M. R., Park, J. H., Jung, K. H., Chai, Y. G."Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure". Oncology Reports 29.3 (2013): 868-874.
Chicago
Lee, H. T., Kim, S. K., Choi, M. R., Park, J. H., Jung, K. H., Chai, Y. G."Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure". Oncology Reports 29, no. 3 (2013): 868-874. https://doi.org/10.3892/or.2012.2209