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Article

Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis

  • Authors:
    • Kun Hao
    • Xiao-Dong Tian
    • Chang-Fu Qin
    • Xue-Hai Xie
    • Yin-Mo Yang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Peking University First Hospital, Beijing 100034, P.R. China
  • Pages: 1124-1132
    |
    Published online on: December 24, 2012
       https://doi.org/10.3892/or.2012.2210
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Abstract

Pancreatic cancer is one of the most aggressive and devastating malignancies. The Hedgehog (Hh) pathway has been reported to play an important role in pancreatic cancer development and progression. The aim of this study was to examine the activation of the Hh pathway in human pancreatic cancer tissue samples and pancreatic cancer cell lines, and the molecular mechanisms involved in the Hh pathway mediated effects on pancreatic cancer cell proliferation and invasion. The expression levels of Hh molecules in human pancreatic cancer tissue samples and pancreatic cancer cell lines were evaluated using RT-PCR. The role of the Hh pathway in cell proliferation and invasion was evaluated using flow cytometry, MTT, colony formation assays and transwell invasion assays, and the expression of cancer stem cell markers and epithelial-mesenchymal transition (EMT) were evaluated using flow cytometry and RT-PCR. Tumorigenicity assays were used to further investigate the role of the Hh pathway in vivo. Hh molecules were highly expressed in human pancreatic cancer tissue samples and pancreatic cancer cell lines. Inhibition of the Hh pathway notably decreased cell proliferation and induced apoptosis through inhibition of the PI3K/AKT pathway and cancer stem cells. Furthermore, inhibition of the Hh signaling pathway significantly inhibited EMT by suppressing the activation of transcription factors Snail and Slug, which are correlated with significantly reduced pancreatic cancer cell invasion, suggesting that the Hh signaling pathway is involved in early metastasis. These results indicate that activation of the Hh pathway is a common event. Inhibition of the Hh pathway may be a potential molecular target of new therapeutic strategies for pancreatic cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Hao K, Tian X, Qin C, Xie X and Yang Y: Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis. Oncol Rep 29: 1124-1132, 2013.
APA
Hao, K., Tian, X., Qin, C., Xie, X., & Yang, Y. (2013). Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis. Oncology Reports, 29, 1124-1132. https://doi.org/10.3892/or.2012.2210
MLA
Hao, K., Tian, X., Qin, C., Xie, X., Yang, Y."Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis". Oncology Reports 29.3 (2013): 1124-1132.
Chicago
Hao, K., Tian, X., Qin, C., Xie, X., Yang, Y."Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis". Oncology Reports 29, no. 3 (2013): 1124-1132. https://doi.org/10.3892/or.2012.2210
Copy and paste a formatted citation
x
Spandidos Publications style
Hao K, Tian X, Qin C, Xie X and Yang Y: Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis. Oncol Rep 29: 1124-1132, 2013.
APA
Hao, K., Tian, X., Qin, C., Xie, X., & Yang, Y. (2013). Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis. Oncology Reports, 29, 1124-1132. https://doi.org/10.3892/or.2012.2210
MLA
Hao, K., Tian, X., Qin, C., Xie, X., Yang, Y."Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis". Oncology Reports 29.3 (2013): 1124-1132.
Chicago
Hao, K., Tian, X., Qin, C., Xie, X., Yang, Y."Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis". Oncology Reports 29, no. 3 (2013): 1124-1132. https://doi.org/10.3892/or.2012.2210
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