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Article

Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria

  • Authors:
    • Alexandre Harlé
    • Maëva Lion
    • Nicolas Lozano
    • Marie Husson
    • Valentin Harter
    • Pascal Genin
    • Jean-Louis Merlin
  • View Affiliations / Copyright

    Affiliations: Department of Biopathology, Centre Alexis Vautrin, F-54519 Vandoeuvre-lès-Nancy Cedex, France, Department of Biostatistics, Centre Alexis Vautrin, F-54519 Vandoeuvre-lès-Nancy Cedex, France
  • Pages: 1043-1052
    |
    Published online on: January 8, 2013
       https://doi.org/10.3892/or.2013.2229
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Abstract

Phosphatidylinositol-3-kinases (PI3K) are essential for cell signaling, proliferation, differentiation and survival. The catalytic subunit of PI3K, encoded by the PIK3CA oncogene, is mutated in 18-45% of breast carcinomas. These mutations, involved in tumorigenic processes, activate the PI3K/AKT/mTOR signaling pathway. Resistance to anti‑human epidermal growth factor receptor, hormonal or anti-PI3K therapies have been described in breast carcinomas bearing activation of the PI3K signaling pathway. The present study reports the evaluation of PIK3CA exon 9 and 20 mutations in 149 invasive breast cancer cases using a validated PCR-high resolution melting assay (PCR-HRM). An amplification refractory mutation system (PCR-ARMS) using allele-specific scorpion primers was used to detect hotspot mutations in exons 9 (c.1624G→A and c.1633G→A) and 20 (c.3140A→G and c.3140A→T) in 118 tumor specimens. No correlation was observed with age at diagnosis, histological type, hormone receptor and HER2 status. PIK3CA exon 9 and 20 mutations were found to be related to Scarff-Bloom-Richardson (SBR) grade with a lower rate of mutations and a higher frequency of exon 9 mutations in SBRI and exon 20 mutations in SBRII/III tumors. No difference was observed in the incidence rates of the two different mutations screened for each exon in any subcategory. A statistically significant correlation was found between PCR-HRM and PCR-ARMS (κ=0.845; P<0.001). PCR-ARMS was found to be more sensitive than PCR-HRM (sensitivity 0.5 and 5-10% of mutated DNA, respectively). We propose that PCR-HRM and PCR-ARMS can be combined for the cost-effective routine clinical identification of PIK3CA mutations for the purpose of personalizing therapy for invasive breast cancers.
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Copy and paste a formatted citation
Spandidos Publications style
Harlé A, Lion M, Lozano N, Husson M, Harter V, Genin P and Merlin J: Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria. Oncol Rep 29: 1043-1052, 2013.
APA
Harlé, A., Lion, M., Lozano, N., Husson, M., Harter, V., Genin, P., & Merlin, J. (2013). Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria. Oncology Reports, 29, 1043-1052. https://doi.org/10.3892/or.2013.2229
MLA
Harlé, A., Lion, M., Lozano, N., Husson, M., Harter, V., Genin, P., Merlin, J."Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria". Oncology Reports 29.3 (2013): 1043-1052.
Chicago
Harlé, A., Lion, M., Lozano, N., Husson, M., Harter, V., Genin, P., Merlin, J."Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria". Oncology Reports 29, no. 3 (2013): 1043-1052. https://doi.org/10.3892/or.2013.2229
Copy and paste a formatted citation
x
Spandidos Publications style
Harlé A, Lion M, Lozano N, Husson M, Harter V, Genin P and Merlin J: Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria. Oncol Rep 29: 1043-1052, 2013.
APA
Harlé, A., Lion, M., Lozano, N., Husson, M., Harter, V., Genin, P., & Merlin, J. (2013). Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria. Oncology Reports, 29, 1043-1052. https://doi.org/10.3892/or.2013.2229
MLA
Harlé, A., Lion, M., Lozano, N., Husson, M., Harter, V., Genin, P., Merlin, J."Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria". Oncology Reports 29.3 (2013): 1043-1052.
Chicago
Harlé, A., Lion, M., Lozano, N., Husson, M., Harter, V., Genin, P., Merlin, J."Analysis of PIK3CA exon 9 and 20 mutations in breast cancers using PCR-HRM and PCR-ARMS: Correlation with clinicopathological criteria". Oncology Reports 29, no. 3 (2013): 1043-1052. https://doi.org/10.3892/or.2013.2229
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